| Summary: | The student, Philip Olivares, submitted this Dissertation for approval on 2019-03-11 at 16:15. This Dissertation was approved for publication on 2019-03-13 at 11:14. DSpace SAF Submission Ingestion Package generated from Vireo submission #13422 on 2019-08-22 at 16:20:19 Made available in DSpace on 2019-08-23T20:44:34Z (GMT). No. of bitstreams: 2 OLIVARES-DISSERTATION-2019.pdf: 6438596 bytes, checksum: 97b182c25594e03fe8c55b64f2b30180 (MD5) LICENSE.txt: 4212 bytes, checksum: de8f9cd7ad3410af60aa430e30c898a9 (MD5) Previous issue date: 2019-03-13 Embargo set by: Seth Robbins for item 112261 Lift date: 2021-08-23T20:44:50Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 112261 Lift date: 2021-08-23T20:46:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system The ability for an organism to maintain homeostasis is of the utmost importance from the smallest single-celled lifeform, to the largest blue whale. While the needs of these organisms may vary widely based on their environments, analogous processes are at play. In this thesis, I will describe my doctoral work towards structurally and functionally understanding how a few of these systems, from single celled prokaryotes like streptomycetes and pseudomonads to an Antarctic fish, allow these organisms to survive and thrive in their respectively harsh environments. Starting on the smallest size scale and the uptake of nutrients for use in central metabolism by a single-celled organism from its environment, I investigate a solute binding protein called HtxB from Pseudomonas stutzeri WM88. This protein has been show to be involved in the uptake of reduced phosphorus compounds such as phosphite and hypophosphite when the more widely available phosphate is not available for utilization as a phosphorus source. Phosphorus, being needed for the synthesis of many biomolecules such as phospholipids, DNA, RNA, and ATP, can easily be the mass limiting element halting further ...
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