Investigation of Orc6- and ORCA-interacting Proteins
The Origin Recognition Complex (ORC) binds to the origin of replication and recruits the pre-replication complex to initiate DNA replication in all eukaryotes. The smallest subunit of ORC, Orc6, is essential in DNA replication and mitosis. Additionally, Origin Recognition Complex-Associated (ORCA) i...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Still Image |
Language: | unknown |
Published: |
2018
|
Subjects: | |
Online Access: | http://hdl.handle.net/2142/100019 |
id |
ftunivillidea:oai:www.ideals.illinois.edu:2142/100019 |
---|---|
record_format |
openpolar |
spelling |
ftunivillidea:oai:www.ideals.illinois.edu:2142/100019 2023-05-15T17:52:58+02:00 Investigation of Orc6- and ORCA-interacting Proteins Adusumilli, Sneha Lin, Yo-Chuen Prasanth, Supriya G. 2018-04 http://hdl.handle.net/2142/100019 unknown http://hdl.handle.net/2142/100019 Copyright 2018 Sneha Adusumilli Copyright 2018 Yo-Chuen Lin Cell and Developmental Biology Orc6 ORCA BioID BirA fusion protein still image conference poster 2018 ftunivillidea 2018-07-07T22:30:56Z The Origin Recognition Complex (ORC) binds to the origin of replication and recruits the pre-replication complex to initiate DNA replication in all eukaryotes. The smallest subunit of ORC, Orc6, is essential in DNA replication and mitosis. Additionally, Origin Recognition Complex-Associated (ORCA) is a pre-replication complex protein which localizes to regions of heterochromatin and stabilizes ORC. The aim of this study was to further define the role of Orc6 and ORCA by identifying unknown proteins which interact with them. With this goal, we utilized the BioID approach, which involved the expression of fusion proteins containing the BirA biotin ligase. We therefore expressed the BirA-HA-Orc6, BirA-HA-ORCA, or BirA-HA-EYFP fusion protein in mammalian cells and potential Orc6- and ORCA-interacting proteins were labeled upon subsequent addition of biotin to the cells. Finally, we performed a pull-down of biotinylated proteins and mass spectrometry analysis is pending for the pull-down products. Open Still Image Orca University of Illinois at Urbana-Champaign: IDEALS (Illinois Digital Environment for Access to Learning and Scholarship) |
institution |
Open Polar |
collection |
University of Illinois at Urbana-Champaign: IDEALS (Illinois Digital Environment for Access to Learning and Scholarship) |
op_collection_id |
ftunivillidea |
language |
unknown |
topic |
Cell and Developmental Biology Orc6 ORCA BioID BirA fusion protein |
spellingShingle |
Cell and Developmental Biology Orc6 ORCA BioID BirA fusion protein Adusumilli, Sneha Lin, Yo-Chuen Investigation of Orc6- and ORCA-interacting Proteins |
topic_facet |
Cell and Developmental Biology Orc6 ORCA BioID BirA fusion protein |
description |
The Origin Recognition Complex (ORC) binds to the origin of replication and recruits the pre-replication complex to initiate DNA replication in all eukaryotes. The smallest subunit of ORC, Orc6, is essential in DNA replication and mitosis. Additionally, Origin Recognition Complex-Associated (ORCA) is a pre-replication complex protein which localizes to regions of heterochromatin and stabilizes ORC. The aim of this study was to further define the role of Orc6 and ORCA by identifying unknown proteins which interact with them. With this goal, we utilized the BioID approach, which involved the expression of fusion proteins containing the BirA biotin ligase. We therefore expressed the BirA-HA-Orc6, BirA-HA-ORCA, or BirA-HA-EYFP fusion protein in mammalian cells and potential Orc6- and ORCA-interacting proteins were labeled upon subsequent addition of biotin to the cells. Finally, we performed a pull-down of biotinylated proteins and mass spectrometry analysis is pending for the pull-down products. Open |
author2 |
Prasanth, Supriya G. |
format |
Still Image |
author |
Adusumilli, Sneha Lin, Yo-Chuen |
author_facet |
Adusumilli, Sneha Lin, Yo-Chuen |
author_sort |
Adusumilli, Sneha |
title |
Investigation of Orc6- and ORCA-interacting Proteins |
title_short |
Investigation of Orc6- and ORCA-interacting Proteins |
title_full |
Investigation of Orc6- and ORCA-interacting Proteins |
title_fullStr |
Investigation of Orc6- and ORCA-interacting Proteins |
title_full_unstemmed |
Investigation of Orc6- and ORCA-interacting Proteins |
title_sort |
investigation of orc6- and orca-interacting proteins |
publishDate |
2018 |
url |
http://hdl.handle.net/2142/100019 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
http://hdl.handle.net/2142/100019 |
op_rights |
Copyright 2018 Sneha Adusumilli Copyright 2018 Yo-Chuen Lin |
_version_ |
1766160723092701184 |