Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy
© 2017 Elsevier Ltd Many studies predict negative effects of ocean acidification on marine organisms, potentially leading to loss of biodiversity and ecosystem function. Research on species inhabiting naturally high pCO2 environments, such as volcanic CO2 vents, offers an opportunity to understand t...
Published in: | Estuarine, Coastal and Shelf Science |
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ftunivhullir:oai:hull-repository.worktribe.com:479758 2023-05-15T17:51:33+02:00 Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy Wäge, Janine Rotchell, Jeanette M. Gambi, Maria-Cristina Hardege, Jörg D. Hardege, Jorg 2017-11-13 https://hull-repository.worktribe.com/output/479758 https://doi.org/10.1016/j.ecss.2017.11.012 unknown Elsevier https://hull-repository.worktribe.com/output/479758 Estuarine, Coastal and Shelf Science Volume 207 Pagination 351-358 doi:https://doi.org/10.1016/j.ecss.2017.11.012 0272-7714 doi:10.1016/j.ecss.2017.11.012 Aquatic Science Oceanography Specialist Research - Other Journal Article 2017 ftunivhullir https://doi.org/10.1016/j.ecss.2017.11.012 2023-03-26T20:24:05Z © 2017 Elsevier Ltd Many studies predict negative effects of ocean acidification on marine organisms, potentially leading to loss of biodiversity and ecosystem function. Research on species inhabiting naturally high pCO2 environments, such as volcanic CO2 vents, offers an opportunity to understand the molecular mechanisms involved in high pCO2 regulation. Here we investigate the relative expression of NADH dehydrogenase, sodium-hydrogen antiporter (NHE), carbonic anhydrase (CA) and paramyosin genes from two non-calcifying sibling Nereididae polychaetes species, Platynereis cfr massiliensis, collected in the shallow CO2 vents off Ischia (Italy; 40°43′52.0″N 13°57′46.2″E and 40°43′55.5″N 13°57′48.4″E), and P. dumerilii collected in an area nearby (40°43′34.51″N; 13°57′35.7″E). The origin of the worms was confirmed using restriction enzyme digest. NHE and paramyosin expressions were both significantly increased in P. dumerilii relative to the P. cfr massiliensis vent populations. Furthermore, a seven day laboratory transfer experiment to lower/higher pCO2 conditions was conducted to investigate the effects on the short term gene expression. The transfer experiment of the non-vent worms to high pCO2 conditions showed no significant effect on any of the genes analysed, however, two genes (NADH dehydrogenase and NHE) from worms of the vent population were significantly down-regulated under low pCO2. These findings will help to gain further insights into the cellular mechanisms affected by pCO2 changes in two polychaete species. Article in Journal/Newspaper Ocean acidification University of Hull: Repository@Hull Estuarine, Coastal and Shelf Science 207 351 358 |
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Open Polar |
collection |
University of Hull: Repository@Hull |
op_collection_id |
ftunivhullir |
language |
unknown |
topic |
Aquatic Science Oceanography Specialist Research - Other |
spellingShingle |
Aquatic Science Oceanography Specialist Research - Other Wäge, Janine Rotchell, Jeanette M. Gambi, Maria-Cristina Hardege, Jörg D. Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
topic_facet |
Aquatic Science Oceanography Specialist Research - Other |
description |
© 2017 Elsevier Ltd Many studies predict negative effects of ocean acidification on marine organisms, potentially leading to loss of biodiversity and ecosystem function. Research on species inhabiting naturally high pCO2 environments, such as volcanic CO2 vents, offers an opportunity to understand the molecular mechanisms involved in high pCO2 regulation. Here we investigate the relative expression of NADH dehydrogenase, sodium-hydrogen antiporter (NHE), carbonic anhydrase (CA) and paramyosin genes from two non-calcifying sibling Nereididae polychaetes species, Platynereis cfr massiliensis, collected in the shallow CO2 vents off Ischia (Italy; 40°43′52.0″N 13°57′46.2″E and 40°43′55.5″N 13°57′48.4″E), and P. dumerilii collected in an area nearby (40°43′34.51″N; 13°57′35.7″E). The origin of the worms was confirmed using restriction enzyme digest. NHE and paramyosin expressions were both significantly increased in P. dumerilii relative to the P. cfr massiliensis vent populations. Furthermore, a seven day laboratory transfer experiment to lower/higher pCO2 conditions was conducted to investigate the effects on the short term gene expression. The transfer experiment of the non-vent worms to high pCO2 conditions showed no significant effect on any of the genes analysed, however, two genes (NADH dehydrogenase and NHE) from worms of the vent population were significantly down-regulated under low pCO2. These findings will help to gain further insights into the cellular mechanisms affected by pCO2 changes in two polychaete species. |
author2 |
Hardege, Jorg |
format |
Article in Journal/Newspaper |
author |
Wäge, Janine Rotchell, Jeanette M. Gambi, Maria-Cristina Hardege, Jörg D. |
author_facet |
Wäge, Janine Rotchell, Jeanette M. Gambi, Maria-Cristina Hardege, Jörg D. |
author_sort |
Wäge, Janine |
title |
Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
title_short |
Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
title_full |
Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
title_fullStr |
Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
title_full_unstemmed |
Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy |
title_sort |
target gene expression studies on platynereis dumerilii and platynereis cfr massiliensis at the shallow co2 vents off ischia, italy |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://hull-repository.worktribe.com/output/479758 https://doi.org/10.1016/j.ecss.2017.11.012 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://hull-repository.worktribe.com/output/479758 Estuarine, Coastal and Shelf Science Volume 207 Pagination 351-358 doi:https://doi.org/10.1016/j.ecss.2017.11.012 0272-7714 doi:10.1016/j.ecss.2017.11.012 |
op_doi |
https://doi.org/10.1016/j.ecss.2017.11.012 |
container_title |
Estuarine, Coastal and Shelf Science |
container_volume |
207 |
container_start_page |
351 |
op_container_end_page |
358 |
_version_ |
1766158732175081472 |