Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis
Hypothesis: Biocatalysts are key to the realization of all bioconversions in nature. However, the difficulty of combining the biocatalyst and other chemicals in one system limits their application in artificial reaction systems. Although some effort, such as Pickering interfacial catalysis and enzym...
Published in: | Journal of Colloid and Interface Science |
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Language: | English |
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2023
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Online Access: | https://hull-repository.worktribe.com/file/4316582/1/Accepted%20manuscript https://hull-repository.worktribe.com/output/4316582 https://doi.org/10.1016/j.jcis.2023.06.009 |
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ftunivhullir:oai:hull-repository.worktribe.com:4316582 2024-09-15T17:41:47+00:00 Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis Yin, Zhengqiao Zhou, Yiding Liu, Xiucai Zhang, Shengmiao Binks, Bernard P. 2023-06-04 https://hull-repository.worktribe.com/file/4316582/1/Accepted%20manuscript https://hull-repository.worktribe.com/output/4316582 https://doi.org/10.1016/j.jcis.2023.06.009 English eng Elsevier https://hull-repository.worktribe.com/output/4316582 Journal of Colloid and Interface Science Volume 648 Pagination 308-316 doi:https://doi.org/10.1016/j.jcis.2023.06.009 https://hull-repository.worktribe.com/file/4316582/1/Accepted%20manuscript 0021-9797 doi:10.1016/j.jcis.2023.06.009 openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ Journal Article acceptedVersion 2023 ftunivhullir https://doi.org/10.1016/j.jcis.2023.06.009 2024-07-15T14:12:00Z Hypothesis: Biocatalysts are key to the realization of all bioconversions in nature. However, the difficulty of combining the biocatalyst and other chemicals in one system limits their application in artificial reaction systems. Although some effort, such as Pickering interfacial catalysis and enzyme-immobilized microchannel reactors, have addressed this challenge an effective method to combine chemical substrates and biocatalysts in a highly efficient and re-usable monolith system is still to be developed. Experiments: A repeated batch-type biphasic interfacial biocatalysis microreactor was developed using enzyme-loaded polymersomes in the void surface of porous monoliths. Polymersomes, loaded with Candida antarctica Lipase B (CALB), are fabricated by self-assembly of the copolymer PEO-b-P(St-co-TMI) and used to stabilize oil-in-water (o/w) Pickering emulsions as a template to prepare monoliths. By adding monomer and Tween 85 to the continuous phase, controllable open-cell monoliths are prepared to inlay CALB-loaded polymersomes in the pore walls. Findings: The microreactor is proven to be highly effective and recyclable when a substrate flows through it, which offers superior benefits of absolute separation to a pure product and no enzyme loss. The relative enzyme activity is constantly maintained above 93% in 15 cycles. The enzyme is constantly present in the microenvironment of the PBS buffer ensuring its immunity to inactivation and facilitating its recycling. Article in Journal/Newspaper Antarc* Antarctica University of Hull: Repository@Hull Journal of Colloid and Interface Science 648 308 316 |
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Open Polar |
collection |
University of Hull: Repository@Hull |
op_collection_id |
ftunivhullir |
language |
English |
description |
Hypothesis: Biocatalysts are key to the realization of all bioconversions in nature. However, the difficulty of combining the biocatalyst and other chemicals in one system limits their application in artificial reaction systems. Although some effort, such as Pickering interfacial catalysis and enzyme-immobilized microchannel reactors, have addressed this challenge an effective method to combine chemical substrates and biocatalysts in a highly efficient and re-usable monolith system is still to be developed. Experiments: A repeated batch-type biphasic interfacial biocatalysis microreactor was developed using enzyme-loaded polymersomes in the void surface of porous monoliths. Polymersomes, loaded with Candida antarctica Lipase B (CALB), are fabricated by self-assembly of the copolymer PEO-b-P(St-co-TMI) and used to stabilize oil-in-water (o/w) Pickering emulsions as a template to prepare monoliths. By adding monomer and Tween 85 to the continuous phase, controllable open-cell monoliths are prepared to inlay CALB-loaded polymersomes in the pore walls. Findings: The microreactor is proven to be highly effective and recyclable when a substrate flows through it, which offers superior benefits of absolute separation to a pure product and no enzyme loss. The relative enzyme activity is constantly maintained above 93% in 15 cycles. The enzyme is constantly present in the microenvironment of the PBS buffer ensuring its immunity to inactivation and facilitating its recycling. |
format |
Article in Journal/Newspaper |
author |
Yin, Zhengqiao Zhou, Yiding Liu, Xiucai Zhang, Shengmiao Binks, Bernard P. |
spellingShingle |
Yin, Zhengqiao Zhou, Yiding Liu, Xiucai Zhang, Shengmiao Binks, Bernard P. Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
author_facet |
Yin, Zhengqiao Zhou, Yiding Liu, Xiucai Zhang, Shengmiao Binks, Bernard P. |
author_sort |
Yin, Zhengqiao |
title |
Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
title_short |
Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
title_full |
Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
title_fullStr |
Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
title_full_unstemmed |
Highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
title_sort |
highly efficient and recyclable monolithic bioreactor for interfacial enzyme catalysis |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://hull-repository.worktribe.com/file/4316582/1/Accepted%20manuscript https://hull-repository.worktribe.com/output/4316582 https://doi.org/10.1016/j.jcis.2023.06.009 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://hull-repository.worktribe.com/output/4316582 Journal of Colloid and Interface Science Volume 648 Pagination 308-316 doi:https://doi.org/10.1016/j.jcis.2023.06.009 https://hull-repository.worktribe.com/file/4316582/1/Accepted%20manuscript 0021-9797 doi:10.1016/j.jcis.2023.06.009 |
op_rights |
openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1016/j.jcis.2023.06.009 |
container_title |
Journal of Colloid and Interface Science |
container_volume |
648 |
container_start_page |
308 |
op_container_end_page |
316 |
_version_ |
1810488039983546368 |