Time should be considered in developmental ecotoxicity test
Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the pr...
Published in: | Marine Pollution Bulletin |
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Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
2002
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Online Access: | https://doi.org/10.1016/S0025-326X(02)00116-9 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+tests http://hdl.handle.net/10722/73249 |
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ftunivhongkonghu:oai:hub.hku.hk:10722/73249 2023-05-15T15:58:47+02:00 Time should be considered in developmental ecotoxicity test Grist, EPM Leung, KMY Worboys, MA Crane, M 2002 https://doi.org/10.1016/S0025-326X(02)00116-9 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+tests http://hdl.handle.net/10722/73249 eng eng Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul United Kingdom Marine Pollution Bulletin http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036741525&selection=ref&src=s&origin=recordpage The 3ird International Conference on Marine Pollution and Ecotoxicology, Hong Kong, 10–14th June 2001. In Marine Pollution Bulletin, 2002, v. 45 n. 1-12, p. 92-99 doi:10.1016/S0025-326X(02)00116-9 99 76102 WOS:000178336300012 0025-326X 1-12 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+tests 12398372 eid_2-s2.0-0036741525 92 http://hdl.handle.net/10722/73249 45 Conference_Paper 2002 ftunivhongkonghu https://doi.org/10.1016/S0025-326X(02)00116-9 2023-01-14T15:15:00Z Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO 4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 μgl -1 (at 20°C, salinity 35‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24-72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0-32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. © 2002 Elsevier Science Ltd. All rights reserved. link_to_subscribed_fulltext Conference Object Crassostrea gigas Pacific oyster University of Hong Kong: HKU Scholars Hub Pacific Marine Pollution Bulletin 45 1-12 92 99 |
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Open Polar |
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University of Hong Kong: HKU Scholars Hub |
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ftunivhongkonghu |
language |
English |
description |
Developmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO 4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 μgl -1 (at 20°C, salinity 35‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24-72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0-32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. © 2002 Elsevier Science Ltd. All rights reserved. link_to_subscribed_fulltext |
format |
Conference Object |
author |
Grist, EPM Leung, KMY Worboys, MA Crane, M |
spellingShingle |
Grist, EPM Leung, KMY Worboys, MA Crane, M Time should be considered in developmental ecotoxicity test |
author_facet |
Grist, EPM Leung, KMY Worboys, MA Crane, M |
author_sort |
Grist, EPM |
title |
Time should be considered in developmental ecotoxicity test |
title_short |
Time should be considered in developmental ecotoxicity test |
title_full |
Time should be considered in developmental ecotoxicity test |
title_fullStr |
Time should be considered in developmental ecotoxicity test |
title_full_unstemmed |
Time should be considered in developmental ecotoxicity test |
title_sort |
time should be considered in developmental ecotoxicity test |
publisher |
Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul |
publishDate |
2002 |
url |
https://doi.org/10.1016/S0025-326X(02)00116-9 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+tests http://hdl.handle.net/10722/73249 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
Marine Pollution Bulletin http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036741525&selection=ref&src=s&origin=recordpage The 3ird International Conference on Marine Pollution and Ecotoxicology, Hong Kong, 10–14th June 2001. In Marine Pollution Bulletin, 2002, v. 45 n. 1-12, p. 92-99 doi:10.1016/S0025-326X(02)00116-9 99 76102 WOS:000178336300012 0025-326X 1-12 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+tests 12398372 eid_2-s2.0-0036741525 92 http://hdl.handle.net/10722/73249 45 |
op_doi |
https://doi.org/10.1016/S0025-326X(02)00116-9 |
container_title |
Marine Pollution Bulletin |
container_volume |
45 |
container_issue |
1-12 |
container_start_page |
92 |
op_container_end_page |
99 |
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1766394554578108416 |