Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices
Aims: To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters. Methods and results: By immunohistochemistry, both cytoplasmic and nucle...
Published in: | Histopathology |
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Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS
2008
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Online Access: | https://doi.org/10.1111/j.1365-2559.2008.03089.x http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indices http://hdl.handle.net/10722/60356 |
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ftunivhongkonghu:oai:hub.hku.hk:10722/60356 2023-05-15T16:32:13+02:00 Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices Ngan, HYS Wong, ESY Zhang, HJ Chan, HY Cheung, ANY Chan, KYK Siu, MKY 2008 https://doi.org/10.1111/j.1365-2559.2008.03089.x http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indices http://hdl.handle.net/10722/60356 eng eng Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS United Kingdom Histopathology Stem cell related genes in gestational trophoblastic diseases http://www.scopus.com/mlt/select.url?eid=2-s2.0-47749120647&selection=ref&src=s&origin=recordpage Histopathology, 2008, v. 53 n. 2, p. 139-146 3044466 doi:10.1111/j.1365-2559.2008.03089.x 146 150697 WOS:000258504400002 0309-0167 2 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indices 18752497 eid_2-s2.0-47749120647 139 http://hdl.handle.net/10722/60356 53 Histopathology. Copyright © Blackwell Publishing Ltd. Gestational trophoblastic disease Stat3 Uterine Neoplasms - genetics - metabolism - pathology Trophoblastic Tumor Placental Site - genetics - metabolism - pathology STAT3 Transcription Factor - biosynthesis - genetics - metabolism Pregnancy Phosphorylation Immunohistochemistry Humans Female Cell Line Tumor Apoptosis - genetics - physiology Article 2008 ftunivhongkonghu https://doi.org/10.1111/j.1365-2559.2008.03089.x 2023-01-14T15:10:45Z Aims: To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters. Methods and results: By immunohistochemistry, both cytoplasmic and nuclear expression of p-Stat3-Ser 727 was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Nuclear immunoreactivity of p-Stat3-Ser 727 was significantly higher in hydatidiform mole (HM) (P < 0.001) and choriocarcinoma (P = 0.009) when compared with normal placentas. Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p-Stat3-Ser 727 expression than their normal trophoblast counterparts. Higher p-Stat3-Ser 727 expression was confirmed in choriocarcinoma cell lines, JEG-3 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p-Stat3-Ser 727 immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (P = 0.013, P = 0.039). There was a significant positive and inverse correlation between nuclear p-Stat3-Ser 727 immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (P = 0.001, P < 0.001, Spearman's ρ test) and Bcl-2 expression (P = 0.034), respectively. Conclusions: p-Stat3-Ser 727 plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. p-Stat3-Ser 727 immunoreactivity is a potential marker in predicting GTN in HM. © 2008 The Authors. link_to_subscribed_fulltext Article in Journal/Newspaper GTN-P University of Hong Kong: HKU Scholars Hub Histopathology 53 2 139 146 |
institution |
Open Polar |
collection |
University of Hong Kong: HKU Scholars Hub |
op_collection_id |
ftunivhongkonghu |
language |
English |
topic |
Gestational trophoblastic disease Stat3 Uterine Neoplasms - genetics - metabolism - pathology Trophoblastic Tumor Placental Site - genetics - metabolism - pathology STAT3 Transcription Factor - biosynthesis - genetics - metabolism Pregnancy Phosphorylation Immunohistochemistry Humans Female Cell Line Tumor Apoptosis - genetics - physiology |
spellingShingle |
Gestational trophoblastic disease Stat3 Uterine Neoplasms - genetics - metabolism - pathology Trophoblastic Tumor Placental Site - genetics - metabolism - pathology STAT3 Transcription Factor - biosynthesis - genetics - metabolism Pregnancy Phosphorylation Immunohistochemistry Humans Female Cell Line Tumor Apoptosis - genetics - physiology Ngan, HYS Wong, ESY Zhang, HJ Chan, HY Cheung, ANY Chan, KYK Siu, MKY Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
topic_facet |
Gestational trophoblastic disease Stat3 Uterine Neoplasms - genetics - metabolism - pathology Trophoblastic Tumor Placental Site - genetics - metabolism - pathology STAT3 Transcription Factor - biosynthesis - genetics - metabolism Pregnancy Phosphorylation Immunohistochemistry Humans Female Cell Line Tumor Apoptosis - genetics - physiology |
description |
Aims: To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters. Methods and results: By immunohistochemistry, both cytoplasmic and nuclear expression of p-Stat3-Ser 727 was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Nuclear immunoreactivity of p-Stat3-Ser 727 was significantly higher in hydatidiform mole (HM) (P < 0.001) and choriocarcinoma (P = 0.009) when compared with normal placentas. Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p-Stat3-Ser 727 expression than their normal trophoblast counterparts. Higher p-Stat3-Ser 727 expression was confirmed in choriocarcinoma cell lines, JEG-3 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p-Stat3-Ser 727 immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (P = 0.013, P = 0.039). There was a significant positive and inverse correlation between nuclear p-Stat3-Ser 727 immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (P = 0.001, P < 0.001, Spearman's ρ test) and Bcl-2 expression (P = 0.034), respectively. Conclusions: p-Stat3-Ser 727 plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. p-Stat3-Ser 727 immunoreactivity is a potential marker in predicting GTN in HM. © 2008 The Authors. link_to_subscribed_fulltext |
format |
Article in Journal/Newspaper |
author |
Ngan, HYS Wong, ESY Zhang, HJ Chan, HY Cheung, ANY Chan, KYK Siu, MKY |
author_facet |
Ngan, HYS Wong, ESY Zhang, HJ Chan, HY Cheung, ANY Chan, KYK Siu, MKY |
author_sort |
Ngan, HYS |
title |
Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
title_short |
Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
title_full |
Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
title_fullStr |
Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
title_full_unstemmed |
Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices |
title_sort |
activated stat3 expression in gestational trophoblastic disease: correlation with clinicopathological parameters and apoptotic indices |
publisher |
Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS |
publishDate |
2008 |
url |
https://doi.org/10.1111/j.1365-2559.2008.03089.x http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indices http://hdl.handle.net/10722/60356 |
genre |
GTN-P |
genre_facet |
GTN-P |
op_relation |
Histopathology Stem cell related genes in gestational trophoblastic diseases http://www.scopus.com/mlt/select.url?eid=2-s2.0-47749120647&selection=ref&src=s&origin=recordpage Histopathology, 2008, v. 53 n. 2, p. 139-146 3044466 doi:10.1111/j.1365-2559.2008.03089.x 146 150697 WOS:000258504400002 0309-0167 2 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indices 18752497 eid_2-s2.0-47749120647 139 http://hdl.handle.net/10722/60356 53 |
op_rights |
Histopathology. Copyright © Blackwell Publishing Ltd. |
op_doi |
https://doi.org/10.1111/j.1365-2559.2008.03089.x |
container_title |
Histopathology |
container_volume |
53 |
container_issue |
2 |
container_start_page |
139 |
op_container_end_page |
146 |
_version_ |
1766021974350364672 |