Low-diversity fungal assemblage in an Antarctic Dry Valleys soil
The McMurdo Dry Valleys of Antarctica present extreme environmental challenges. Life is restricted to patchy occurrence of lichens, mosses and invertebrates, plus soil microbial communities. Fungi have been described in lichen symbioses but relatively little is known about the occurrence of free-liv...
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Language: | English |
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Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00300/index.htm
2012
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Online Access: | https://doi.org/10.1007/s00300-011-1102-2 http://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, et al. http://hdl.handle.net/10722/144937 |
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ftunivhongkonghu:oai:hub.hku.hk:10722/144937 2023-05-15T13:46:34+02:00 Low-diversity fungal assemblage in an Antarctic Dry Valleys soil Farrell, RL Hyde, KD Pointing, SB Lacap, DC Rao, S Chan, Y 2012 https://doi.org/10.1007/s00300-011-1102-2 http://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, et al. http://hdl.handle.net/10722/144937 eng eng Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00300/index.htm Germany Polar Biology http://www.scopus.com/mlt/select.url?eid=2-s2.0-84858006401&selection=ref&src=s&origin=recordpage Polar Biology, 2012, v. 35 n. 4, p. 567-574 9842925 doi:10.1007/s00300-011-1102-2 1432-2056 574 218930 WOS:000301451600009 0722-4060 4 http://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, et al. eid_2-s2.0-84858006401 567 http://hdl.handle.net/10722/144937 35 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The Author(s) CC-BY-NC-ND Antarctica Soil fungi Debaryomyces Zalerion Helicodendron Article 2012 ftunivhongkonghu https://doi.org/10.1007/s00300-011-1102-2 2023-01-14T15:41:06Z The McMurdo Dry Valleys of Antarctica present extreme environmental challenges. Life is restricted to patchy occurrence of lichens, mosses and invertebrates, plus soil microbial communities. Fungi have been described in lichen symbioses but relatively little is known about the occurrence of free-living soil fungi in the Dry Valleys. A challenge in estimating fungal species richness has been the extent to which estimates based on either cultivation or environmental DNA reflect the active assemblage in cold-arid soils. Here, we describe analysis for inland Dry Valleys soil of environmental DNA and RNA (cDNA) to infer total and putative metabolically active assemblages, respectively, plus cultivation approaches using a variety of laboratory growth conditions. Environmental sequences indicated a highly restricted assemblage of just seven phylotypes that affiliated phylogenetically within two known genera, Helicodendron and Zalerion, plus previously unidentified fungal phylotypes. None of the commonly encountered molds and mitosporic genera recorded from maritime Antarctic locations were encountered. A striking difference was observed in the frequency of recovery for phylotypes between libraries. This suggests that both species richness and beta diversity estimates based on DNA libraries have the potential to misinform putatively active assemblages. Cultivation yielded a cold-tolerant Zalerion strain that affiliated with DNA and RNA library clones, and a psychrotrophic yeast (Debaryomyces hansenii), which was not detected using either culture-independent approach. © 2011 The Author(s). published_or_final_version Springer Open Choice, 21 Feb 2012 Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Polar Biology University of Hong Kong: HKU Scholars Hub Antarctic McMurdo Dry Valleys Polar Biology 35 4 567 574 |
institution |
Open Polar |
collection |
University of Hong Kong: HKU Scholars Hub |
op_collection_id |
ftunivhongkonghu |
language |
English |
topic |
Antarctica Soil fungi Debaryomyces Zalerion Helicodendron |
spellingShingle |
Antarctica Soil fungi Debaryomyces Zalerion Helicodendron Farrell, RL Hyde, KD Pointing, SB Lacap, DC Rao, S Chan, Y Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
topic_facet |
Antarctica Soil fungi Debaryomyces Zalerion Helicodendron |
description |
The McMurdo Dry Valleys of Antarctica present extreme environmental challenges. Life is restricted to patchy occurrence of lichens, mosses and invertebrates, plus soil microbial communities. Fungi have been described in lichen symbioses but relatively little is known about the occurrence of free-living soil fungi in the Dry Valleys. A challenge in estimating fungal species richness has been the extent to which estimates based on either cultivation or environmental DNA reflect the active assemblage in cold-arid soils. Here, we describe analysis for inland Dry Valleys soil of environmental DNA and RNA (cDNA) to infer total and putative metabolically active assemblages, respectively, plus cultivation approaches using a variety of laboratory growth conditions. Environmental sequences indicated a highly restricted assemblage of just seven phylotypes that affiliated phylogenetically within two known genera, Helicodendron and Zalerion, plus previously unidentified fungal phylotypes. None of the commonly encountered molds and mitosporic genera recorded from maritime Antarctic locations were encountered. A striking difference was observed in the frequency of recovery for phylotypes between libraries. This suggests that both species richness and beta diversity estimates based on DNA libraries have the potential to misinform putatively active assemblages. Cultivation yielded a cold-tolerant Zalerion strain that affiliated with DNA and RNA library clones, and a psychrotrophic yeast (Debaryomyces hansenii), which was not detected using either culture-independent approach. © 2011 The Author(s). published_or_final_version Springer Open Choice, 21 Feb 2012 |
format |
Article in Journal/Newspaper |
author |
Farrell, RL Hyde, KD Pointing, SB Lacap, DC Rao, S Chan, Y |
author_facet |
Farrell, RL Hyde, KD Pointing, SB Lacap, DC Rao, S Chan, Y |
author_sort |
Farrell, RL |
title |
Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
title_short |
Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
title_full |
Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
title_fullStr |
Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
title_full_unstemmed |
Low-diversity fungal assemblage in an Antarctic Dry Valleys soil |
title_sort |
low-diversity fungal assemblage in an antarctic dry valleys soil |
publisher |
Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00300/index.htm |
publishDate |
2012 |
url |
https://doi.org/10.1007/s00300-011-1102-2 http://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, et al. http://hdl.handle.net/10722/144937 |
geographic |
Antarctic McMurdo Dry Valleys |
geographic_facet |
Antarctic McMurdo Dry Valleys |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Polar Biology |
op_relation |
Polar Biology http://www.scopus.com/mlt/select.url?eid=2-s2.0-84858006401&selection=ref&src=s&origin=recordpage Polar Biology, 2012, v. 35 n. 4, p. 567-574 9842925 doi:10.1007/s00300-011-1102-2 1432-2056 574 218930 WOS:000301451600009 0722-4060 4 http://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, et al. eid_2-s2.0-84858006401 567 http://hdl.handle.net/10722/144937 35 |
op_rights |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The Author(s) |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1007/s00300-011-1102-2 |
container_title |
Polar Biology |
container_volume |
35 |
container_issue |
4 |
container_start_page |
567 |
op_container_end_page |
574 |
_version_ |
1766244220152053760 |