Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid
Myeloid differentiation factor 88 (MyD88) is a key and essential adapter involved in the interleukin-1 receptor (IL-1R) and toll-like receptor (TLR)-mediated activation signaling pathway. To investigate molecular characterization of MyD88 and its gene expression profile in response to stimulation by...
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ftunivhmevols:oai:evols.library.manoa.hawaii.edu:10524/54942 2023-05-15T15:58:40+02:00 Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid Kecheng Zhu Nan Zhang Huayang Guo Shigui Jiang Dianchang Zhang 9 pages http://hdl.handle.net/10524/54942 unknown The Israeli Journal of Aquaculture - Bamidgeh Pinctada fucata MyD88 LPS and poly (I: C) gene expression Fish culture--Israel. Fish culture. Article Text ftunivhmevols 2019-01-02T18:06:17Z Myeloid differentiation factor 88 (MyD88) is a key and essential adapter involved in the interleukin-1 receptor (IL-1R) and toll-like receptor (TLR)-mediated activation signaling pathway. To investigate molecular characterization of MyD88 and its gene expression profile in response to stimulation by lipopolysaccharide (LPS) and polyinosinic-cytidylic acid (poly (I: C)), we isolated the MyD88 cDNA sequence in Pinctada fucata and analyzed expression patterns using quantitative real-time PCR. Sequence analysis indicated that Pf-MyD88 cDNA is 1463bp in length and contains a1050bp open reading frame that encodes a 349 α peptide. Pf-MyD88 has the highest similarity with homologues of Crassostrea gigas and highly conserved death and toll/IL-1R domains. Furthermore, during LPS and poly (I:C)-stimulated experiments in the gill, peak expression levels of Pf-MyD88 were detected at 2h and 8h with a 1.58-fold and 3.58-fold increase, respectively. The results revealed the existence of a MyD88-dependent signaling pathway in P. fucata and contributed to understanding the potential role of Pf-MyD88 in the TLR/IL-1R-mediated signaling pathway. Article in Journal/Newspaper Crassostrea gigas Digital Repository of the University of Hawaii at Manoa |
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Open Polar |
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Digital Repository of the University of Hawaii at Manoa |
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ftunivhmevols |
language |
unknown |
topic |
Pinctada fucata MyD88 LPS and poly (I: C) gene expression Fish culture--Israel. Fish culture. |
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Pinctada fucata MyD88 LPS and poly (I: C) gene expression Fish culture--Israel. Fish culture. Kecheng Zhu Nan Zhang Huayang Guo Shigui Jiang Dianchang Zhang Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
topic_facet |
Pinctada fucata MyD88 LPS and poly (I: C) gene expression Fish culture--Israel. Fish culture. |
description |
Myeloid differentiation factor 88 (MyD88) is a key and essential adapter involved in the interleukin-1 receptor (IL-1R) and toll-like receptor (TLR)-mediated activation signaling pathway. To investigate molecular characterization of MyD88 and its gene expression profile in response to stimulation by lipopolysaccharide (LPS) and polyinosinic-cytidylic acid (poly (I: C)), we isolated the MyD88 cDNA sequence in Pinctada fucata and analyzed expression patterns using quantitative real-time PCR. Sequence analysis indicated that Pf-MyD88 cDNA is 1463bp in length and contains a1050bp open reading frame that encodes a 349 α peptide. Pf-MyD88 has the highest similarity with homologues of Crassostrea gigas and highly conserved death and toll/IL-1R domains. Furthermore, during LPS and poly (I:C)-stimulated experiments in the gill, peak expression levels of Pf-MyD88 were detected at 2h and 8h with a 1.58-fold and 3.58-fold increase, respectively. The results revealed the existence of a MyD88-dependent signaling pathway in P. fucata and contributed to understanding the potential role of Pf-MyD88 in the TLR/IL-1R-mediated signaling pathway. |
format |
Article in Journal/Newspaper |
author |
Kecheng Zhu Nan Zhang Huayang Guo Shigui Jiang Dianchang Zhang |
author_facet |
Kecheng Zhu Nan Zhang Huayang Guo Shigui Jiang Dianchang Zhang |
author_sort |
Kecheng Zhu |
title |
Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
title_short |
Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
title_full |
Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
title_fullStr |
Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
title_full_unstemmed |
Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid |
title_sort |
molecular characterization of myd88 in pinctada fucata and its response to lipopolysaccharides and polyinosinic-cytidylic acid |
url |
http://hdl.handle.net/10524/54942 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
The Israeli Journal of Aquaculture - Bamidgeh |
_version_ |
1766394428607430656 |