Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities
The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, caus- ing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of...
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ftunivhmevols:oai:evols.library.manoa.hawaii.edu:10524/19207 2023-05-15T16:08:39+02:00 Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities Xylouri, Eftychia Kotzamanis, Yannis R. Athanassopoulou, Fotini Dong, Li Pappas, Loanis S. Argyrokastritis, Alexandros Fragkiadaki, Eirini 6 pages http://hdl.handle.net/10524/19207 unknown Israeli Journal of Aquaculture - BAMIGDEH The Israeli Journal of Aquaculture - Bamidgeh Acipenser sp. nodavirus isolation characterization sequencing Fish culture--Israel--Periodicals. Fish culture--Periodicals. Aquaculture--Israel--Periodicals. Aquaculture--Periodicals. ftunivhmevols 2019-01-02T17:54:43Z The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, caus- ing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested- PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was hori- zontally transmitted to freshwater sturgeon. Other/Unknown Material European eel Digital Repository of the University of Hawaii at Manoa |
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Open Polar |
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Digital Repository of the University of Hawaii at Manoa |
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ftunivhmevols |
language |
unknown |
topic |
Acipenser sp. nodavirus isolation characterization sequencing Fish culture--Israel--Periodicals. Fish culture--Periodicals. Aquaculture--Israel--Periodicals. Aquaculture--Periodicals. |
spellingShingle |
Acipenser sp. nodavirus isolation characterization sequencing Fish culture--Israel--Periodicals. Fish culture--Periodicals. Aquaculture--Israel--Periodicals. Aquaculture--Periodicals. Xylouri, Eftychia Kotzamanis, Yannis R. Athanassopoulou, Fotini Dong, Li Pappas, Loanis S. Argyrokastritis, Alexandros Fragkiadaki, Eirini Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
topic_facet |
Acipenser sp. nodavirus isolation characterization sequencing Fish culture--Israel--Periodicals. Fish culture--Periodicals. Aquaculture--Israel--Periodicals. Aquaculture--Periodicals. |
description |
The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, caus- ing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested- PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was hori- zontally transmitted to freshwater sturgeon. |
author |
Xylouri, Eftychia Kotzamanis, Yannis R. Athanassopoulou, Fotini Dong, Li Pappas, Loanis S. Argyrokastritis, Alexandros Fragkiadaki, Eirini |
author_facet |
Xylouri, Eftychia Kotzamanis, Yannis R. Athanassopoulou, Fotini Dong, Li Pappas, Loanis S. Argyrokastritis, Alexandros Fragkiadaki, Eirini |
author_sort |
Xylouri, Eftychia |
title |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_short |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_full |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_fullStr |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_full_unstemmed |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_sort |
isolation, characterization, and sequencing of nodavirus in sturgeon (acipenser gueldestaedi l.) reared in freshwater facilities |
publisher |
Israeli Journal of Aquaculture - BAMIGDEH |
url |
http://hdl.handle.net/10524/19207 |
genre |
European eel |
genre_facet |
European eel |
op_relation |
The Israeli Journal of Aquaculture - Bamidgeh |
_version_ |
1766404672614039552 |