Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine
In this study, cotton terry cloth fibrils were coated with 0.2% polyethyleneimine (PEI). Lipases from Candida antarctica A (CALA) and Thermomyces lanuginosus (TL) were immobilized on this support through adsorption followed by cross-linking with 0.2% glutaraldehyde. PEI-enzyme aggregates formation a...
Published in: | Colloids and Surfaces B: Biointerfaces |
---|---|
Main Authors: | , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2012
|
Subjects: | |
Online Access: | https://hdl.handle.net/11491/1080 https://doi.org/10.1016/j.colsurfb.2012.02.020 |
id |
ftunivhitit:oai:earsiv.hitit.edu.tr:11491/1080 |
---|---|
record_format |
openpolar |
spelling |
ftunivhitit:oai:earsiv.hitit.edu.tr:11491/1080 2024-09-15T17:43:29+00:00 Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine Ondul, Eda Dizge, Nadir Albayrak, Nedim Hitit Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü 2012 https://hdl.handle.net/11491/1080 https://doi.org/10.1016/j.colsurfb.2012.02.020 eng eng 10.1016/j.colsurfb.2012.02.020 Colloids and Surfaces B: Biointerfaces MAG-107M487 Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı Ondul, E., Dizge, N., Albayrak, N. (2012). Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine. Colloids and Surfaces B: Biointerfaces, 95, 109-114. 0927-7765 https://doi.org/10.1016/j.colsurfb.2012.02.020 https://hdl.handle.net/11491/1080 95 109 114 info:eu-repo/semantics/closedAccess Candida Antarctica A Lipase Cotton Terry Cloth Fibrils Immobilization Polyethyleneimine Thermomyces Lanuginosus Lipase article 2012 ftunivhitit https://doi.org/10.1016/j.colsurfb.2012.02.020 2024-07-09T03:02:32Z In this study, cotton terry cloth fibrils were coated with 0.2% polyethyleneimine (PEI). Lipases from Candida antarctica A (CALA) and Thermomyces lanuginosus (TL) were immobilized on this support through adsorption followed by cross-linking with 0.2% glutaraldehyde. PEI-enzyme aggregates formation and growth of aggregates on cotton cloth fibrils lead to multilayer immobilization of the lipases. PEI and lipase was mixed to form PEI-enzyme complex/aggregate. The highest amount of enzyme precipitate was obtained at the PEI to enzyme ratio of 1/20-1/40 for both lipases. The effect of pH was also investigated for aggregates formation. The results showed that when pH values were below 8, aggregation and precipitation were not occurred for C. antarctica A lipase. However, pH did not affect PEI-enzyme aggregate formation for T. lanuginosus lipase. Immobilized enzyme amount was approximately 180. mg/g support and 200. mg/g support for T. lanuginosus and C. antarctica A lipases, respectively. Effect of the reaction temperature on the relative activity of the free and immobilized lipases at various temperature (30-80°C) was studied. It was found that immobilization had no effect on the optimum temperature and it was 60°C for both free and immobilized enzymes. The effect of operational and storage stability on activity of free and immobilized lipases were also investigated. Immobilized lipases exhibited that they could be stored at room temperature with a little activity lost during 28 days. © 2012 Elsevier B.V. Article in Journal/Newspaper Antarc* Antarctica Hitit University Institutional Repository (DSpace@Hitit) Colloids and Surfaces B: Biointerfaces 95 109 114 |
institution |
Open Polar |
collection |
Hitit University Institutional Repository (DSpace@Hitit) |
op_collection_id |
ftunivhitit |
language |
English |
topic |
Candida Antarctica A Lipase Cotton Terry Cloth Fibrils Immobilization Polyethyleneimine Thermomyces Lanuginosus Lipase |
spellingShingle |
Candida Antarctica A Lipase Cotton Terry Cloth Fibrils Immobilization Polyethyleneimine Thermomyces Lanuginosus Lipase Ondul, Eda Dizge, Nadir Albayrak, Nedim Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
topic_facet |
Candida Antarctica A Lipase Cotton Terry Cloth Fibrils Immobilization Polyethyleneimine Thermomyces Lanuginosus Lipase |
description |
In this study, cotton terry cloth fibrils were coated with 0.2% polyethyleneimine (PEI). Lipases from Candida antarctica A (CALA) and Thermomyces lanuginosus (TL) were immobilized on this support through adsorption followed by cross-linking with 0.2% glutaraldehyde. PEI-enzyme aggregates formation and growth of aggregates on cotton cloth fibrils lead to multilayer immobilization of the lipases. PEI and lipase was mixed to form PEI-enzyme complex/aggregate. The highest amount of enzyme precipitate was obtained at the PEI to enzyme ratio of 1/20-1/40 for both lipases. The effect of pH was also investigated for aggregates formation. The results showed that when pH values were below 8, aggregation and precipitation were not occurred for C. antarctica A lipase. However, pH did not affect PEI-enzyme aggregate formation for T. lanuginosus lipase. Immobilized enzyme amount was approximately 180. mg/g support and 200. mg/g support for T. lanuginosus and C. antarctica A lipases, respectively. Effect of the reaction temperature on the relative activity of the free and immobilized lipases at various temperature (30-80°C) was studied. It was found that immobilization had no effect on the optimum temperature and it was 60°C for both free and immobilized enzymes. The effect of operational and storage stability on activity of free and immobilized lipases were also investigated. Immobilized lipases exhibited that they could be stored at room temperature with a little activity lost during 28 days. © 2012 Elsevier B.V. |
author2 |
Hitit Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü |
format |
Article in Journal/Newspaper |
author |
Ondul, Eda Dizge, Nadir Albayrak, Nedim |
author_facet |
Ondul, Eda Dizge, Nadir Albayrak, Nedim |
author_sort |
Ondul, Eda |
title |
Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
title_short |
Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
title_full |
Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
title_fullStr |
Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
title_full_unstemmed |
Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
title_sort |
immobilization of candida antarctica a and thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine |
publishDate |
2012 |
url |
https://hdl.handle.net/11491/1080 https://doi.org/10.1016/j.colsurfb.2012.02.020 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
10.1016/j.colsurfb.2012.02.020 Colloids and Surfaces B: Biointerfaces MAG-107M487 Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı Ondul, E., Dizge, N., Albayrak, N. (2012). Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine. Colloids and Surfaces B: Biointerfaces, 95, 109-114. 0927-7765 https://doi.org/10.1016/j.colsurfb.2012.02.020 https://hdl.handle.net/11491/1080 95 109 114 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.colsurfb.2012.02.020 |
container_title |
Colloids and Surfaces B: Biointerfaces |
container_volume |
95 |
container_start_page |
109 |
op_container_end_page |
114 |
_version_ |
1810490468604051456 |