A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon
Age at maturity is a key life history trait and a significant contributor to life history strategy variation. The maturation process is influenced by genetic and environmental factors, but specific causes of variation in maturation timing remain elusive. In many species, the increase in the regulato...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Format: | Article in Journal/Newspaper |
Language: | Finnish |
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2023
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Online Access: | http://hdl.handle.net/10138/566555 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/566555 2024-01-07T09:42:11+01:00 A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon Ahi, Ehsan Pashay Sinclair-Waters, Marion Donner, Iikki Primmer, Craig R. Organismi- ja evoluutiobiologian tutkimusohjelma Evolution, Conservation, and Genomics Biotekniikan instituutti Biotieteet Kestävyystieteen instituutti (HELSUS) 2023-10-28T22:23:32Z 8 application/pdf http://hdl.handle.net/10138/566555 fin fin Elsevier Scientific Publ. Co 10.1016/j.cbpa.2022.111337 Ahi , E P , Sinclair-Waters , M , Donner , I & Primmer , C R 2023 , ' A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon ' , Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology , Vuosikerta. 275 , 111337 . https://doi.org/10.1016/j.cbpa.2022.111337 RIS: urn:C6EB482DE64458814633B9269F0D3F69 ORCID: /0000-0002-3687-8435/work/122645611 ORCID: /0000-0001-7371-4547/work/122646321 ORCID: /0000-0002-6528-1187/work/122646674 49f7e8f0-90a9-407d-9158-74a6317c7a7c http://hdl.handle.net/10138/566555 000890982900001 cc_by_nc_nd openAccess info:eu-repo/semantics/openAccess 1182 Biokemia solu- ja molekyylibiologia Gene regulatory network Atlantic salmon Maturation timing jun sf1 vgll3 Hippo pathway Artikkeli acceptedVersion 2023 ftunivhelsihelda 2023-12-14T00:08:46Z Age at maturity is a key life history trait and a significant contributor to life history strategy variation. The maturation process is influenced by genetic and environmental factors, but specific causes of variation in maturation timing remain elusive. In many species, the increase in the regulatory gonadotropin-releasing hormone 1 (GnRH1) marks the onset of puberty. Atlantic salmon, however, lacks gnrh1 gene, suggesting gnrh3 and/or other regulatory factors are involved in the maturation process. Earlier research in Atlantic salmon has found a strong association between alternative alleles of vgll3 and maturation timing. Recently we reported strong induction of gonadotropin genes (fshb and lhb) in the pituitary of Atlantic salmon homozygous for the early maturation allele (E) of vgll3. The induction of gonadotropins was accompanied by increased expression of their direct upstream regulators, c-jun and sf1 (nr5a1b) but the regulatory connection between vgll3 and these regulators has never been investigated in any organism. In this study, we investigated the potential regulatory connection between vgll3 genotypes and these regulators through a stepwise approach of identifying a gene regulatory network (GRN) containing c-jun and sf1, and transcription factor motif enrichment analysis. We found a GRN containing c-jun with predicted upstream regulators, e2f1, egr1, foxj1 and klf4, to be differentially expressed in the pituitary. Finally, we suggest a vgll3 and Hippo pathway -dependent model for transcriptional regulation of c-jun and sf1 in the pituitary, which may have broader implications across vertebrates. Peer reviewed Article in Journal/Newspaper Atlantic salmon HELDA – University of Helsinki Open Repository Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 275 111337 |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
Finnish |
topic |
1182 Biokemia solu- ja molekyylibiologia Gene regulatory network Atlantic salmon Maturation timing jun sf1 vgll3 Hippo pathway |
spellingShingle |
1182 Biokemia solu- ja molekyylibiologia Gene regulatory network Atlantic salmon Maturation timing jun sf1 vgll3 Hippo pathway Ahi, Ehsan Pashay Sinclair-Waters, Marion Donner, Iikki Primmer, Craig R. A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
topic_facet |
1182 Biokemia solu- ja molekyylibiologia Gene regulatory network Atlantic salmon Maturation timing jun sf1 vgll3 Hippo pathway |
description |
Age at maturity is a key life history trait and a significant contributor to life history strategy variation. The maturation process is influenced by genetic and environmental factors, but specific causes of variation in maturation timing remain elusive. In many species, the increase in the regulatory gonadotropin-releasing hormone 1 (GnRH1) marks the onset of puberty. Atlantic salmon, however, lacks gnrh1 gene, suggesting gnrh3 and/or other regulatory factors are involved in the maturation process. Earlier research in Atlantic salmon has found a strong association between alternative alleles of vgll3 and maturation timing. Recently we reported strong induction of gonadotropin genes (fshb and lhb) in the pituitary of Atlantic salmon homozygous for the early maturation allele (E) of vgll3. The induction of gonadotropins was accompanied by increased expression of their direct upstream regulators, c-jun and sf1 (nr5a1b) but the regulatory connection between vgll3 and these regulators has never been investigated in any organism. In this study, we investigated the potential regulatory connection between vgll3 genotypes and these regulators through a stepwise approach of identifying a gene regulatory network (GRN) containing c-jun and sf1, and transcription factor motif enrichment analysis. We found a GRN containing c-jun with predicted upstream regulators, e2f1, egr1, foxj1 and klf4, to be differentially expressed in the pituitary. Finally, we suggest a vgll3 and Hippo pathway -dependent model for transcriptional regulation of c-jun and sf1 in the pituitary, which may have broader implications across vertebrates. Peer reviewed |
author2 |
Organismi- ja evoluutiobiologian tutkimusohjelma Evolution, Conservation, and Genomics Biotekniikan instituutti Biotieteet Kestävyystieteen instituutti (HELSUS) |
format |
Article in Journal/Newspaper |
author |
Ahi, Ehsan Pashay Sinclair-Waters, Marion Donner, Iikki Primmer, Craig R. |
author_facet |
Ahi, Ehsan Pashay Sinclair-Waters, Marion Donner, Iikki Primmer, Craig R. |
author_sort |
Ahi, Ehsan Pashay |
title |
A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
title_short |
A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
title_full |
A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
title_fullStr |
A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
title_full_unstemmed |
A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon |
title_sort |
pituitary gene network linking vgll3 to regulators of sexual maturation in male atlantic salmon |
publisher |
Elsevier Scientific Publ. Co |
publishDate |
2023 |
url |
http://hdl.handle.net/10138/566555 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
10.1016/j.cbpa.2022.111337 Ahi , E P , Sinclair-Waters , M , Donner , I & Primmer , C R 2023 , ' A pituitary gene network linking vgll3 to regulators of sexual maturation in male Atlantic salmon ' , Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology , Vuosikerta. 275 , 111337 . https://doi.org/10.1016/j.cbpa.2022.111337 RIS: urn:C6EB482DE64458814633B9269F0D3F69 ORCID: /0000-0002-3687-8435/work/122645611 ORCID: /0000-0001-7371-4547/work/122646321 ORCID: /0000-0002-6528-1187/work/122646674 49f7e8f0-90a9-407d-9158-74a6317c7a7c http://hdl.handle.net/10138/566555 000890982900001 |
op_rights |
cc_by_nc_nd openAccess info:eu-repo/semantics/openAccess |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
275 |
container_start_page |
111337 |
_version_ |
1787423107820027904 |