Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water
Highlights •Coliphages decayed 22–27 times, and E. coli 4.8 times slower in 4 °C than 22 °C. •E. coli, enterococci, and som. coliphages were more sensitive to light than temperature. •Bacterial DNA/RNA, and Legionella were more sensitive to temperature than light. •Norovirus GII and F-specific colip...
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2023
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/352618 2023-08-20T04:10:02+02:00 Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water Tiwari, Ananda Kauppinen, Ari Räsänen, Pia Salonen, Jenniina Wessels, Laura Juntunen, Janne Miettinen, Ilkka T. Pitkänen, Tarja The Finnish Environment Institute Suomen ympäristökeskus 2023-01-06T10:08:15Z application/pdf http://hdl.handle.net/10138/352618 eng eng Elsevier BV 160340 10.1016/j.scitotenv.2022.160340 Science of the Total Environment 0048-9697 859 http://hdl.handle.net/10138/352618 URN:NBN:fi-fe202301061727 CC BY 4.0 openAccess intestinal enterococci microbial source tracking markers norovirus culture counts qPCR RT-qPCR coliphages T90 value mikrobiologia suolistomikrobisto Escherichia coli markkerit merkkiaineet virukset viljelmät laskeminen bakteriofagit simulointi subarktinen alue jokivesi A1 Journal article (refereed), original research A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä 2023 ftunivhelsihelda 2023-07-28T06:16:38Z Highlights •Coliphages decayed 22–27 times, and E. coli 4.8 times slower in 4 °C than 22 °C. •E. coli, enterococci, and som. coliphages were more sensitive to light than temperature. •Bacterial DNA/RNA, and Legionella were more sensitive to temperature than light. •Norovirus GII and F-specific coliphages were also more sensitive to temperature. •Indication power of fecal indicators can vary between seasons of the year. Abstract Knowledge of the decay characteristics of health-related microbes in surface waters is important for modeling the transportation of waterborne pathogens and for assessing their public health risks. Although water temperature and light exposure are major factors determining the decay characteristics of enteric microbes in surface waters, such effects have not been well studied in subarctic surface waters. This study comprehensively evaluated the effect of temperature and light on the decay characteristics of health-related microbes [Escherichia coli, enterococci, microbial source tracking markers (GenBac3 & HF183 assays), coliphages (F-specific and somatic), noroviruses GII and Legionella spp.] under simulated subarctic river water conditions. The experiments were conducted in four different laboratory settings (4 °C/dark, 15 °C/dark, 15 °C/light, and 22 °C/light). The T90 values (time required for a 90 % reduction in the population of a target) of all targets were higher under cold and dark (2.6–51.3 days depending upon targets) than under warm and light conditions (0.6–3.5 days). Under 4 °C/dark (simulated winter) water conditions, F-specific coliphages had 27.2 times higher, and coliform bacteria had 3.3 times higher T90 value than under 22 °C/light (simulated summer) water conditions. Bacterial molecular markers also displayed high variation in T90 values, with the greatest difference between 4 °C/dark and 22 °C/light recorded for HF183 DNA (20.6 times) and the lowest difference for EC23S857 RNA (6.6 times). E. coli, intestinal enterococci, and somatic coliphages were relatively ... Article in Journal/Newspaper Subarctic Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
institution |
Open Polar |
collection |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
intestinal enterococci microbial source tracking markers norovirus culture counts qPCR RT-qPCR coliphages T90 value mikrobiologia suolistomikrobisto Escherichia coli markkerit merkkiaineet virukset viljelmät laskeminen bakteriofagit simulointi subarktinen alue jokivesi |
spellingShingle |
intestinal enterococci microbial source tracking markers norovirus culture counts qPCR RT-qPCR coliphages T90 value mikrobiologia suolistomikrobisto Escherichia coli markkerit merkkiaineet virukset viljelmät laskeminen bakteriofagit simulointi subarktinen alue jokivesi Tiwari, Ananda Kauppinen, Ari Räsänen, Pia Salonen, Jenniina Wessels, Laura Juntunen, Janne Miettinen, Ilkka T. Pitkänen, Tarja Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
topic_facet |
intestinal enterococci microbial source tracking markers norovirus culture counts qPCR RT-qPCR coliphages T90 value mikrobiologia suolistomikrobisto Escherichia coli markkerit merkkiaineet virukset viljelmät laskeminen bakteriofagit simulointi subarktinen alue jokivesi |
description |
Highlights •Coliphages decayed 22–27 times, and E. coli 4.8 times slower in 4 °C than 22 °C. •E. coli, enterococci, and som. coliphages were more sensitive to light than temperature. •Bacterial DNA/RNA, and Legionella were more sensitive to temperature than light. •Norovirus GII and F-specific coliphages were also more sensitive to temperature. •Indication power of fecal indicators can vary between seasons of the year. Abstract Knowledge of the decay characteristics of health-related microbes in surface waters is important for modeling the transportation of waterborne pathogens and for assessing their public health risks. Although water temperature and light exposure are major factors determining the decay characteristics of enteric microbes in surface waters, such effects have not been well studied in subarctic surface waters. This study comprehensively evaluated the effect of temperature and light on the decay characteristics of health-related microbes [Escherichia coli, enterococci, microbial source tracking markers (GenBac3 & HF183 assays), coliphages (F-specific and somatic), noroviruses GII and Legionella spp.] under simulated subarctic river water conditions. The experiments were conducted in four different laboratory settings (4 °C/dark, 15 °C/dark, 15 °C/light, and 22 °C/light). The T90 values (time required for a 90 % reduction in the population of a target) of all targets were higher under cold and dark (2.6–51.3 days depending upon targets) than under warm and light conditions (0.6–3.5 days). Under 4 °C/dark (simulated winter) water conditions, F-specific coliphages had 27.2 times higher, and coliform bacteria had 3.3 times higher T90 value than under 22 °C/light (simulated summer) water conditions. Bacterial molecular markers also displayed high variation in T90 values, with the greatest difference between 4 °C/dark and 22 °C/light recorded for HF183 DNA (20.6 times) and the lowest difference for EC23S857 RNA (6.6 times). E. coli, intestinal enterococci, and somatic coliphages were relatively ... |
author2 |
The Finnish Environment Institute Suomen ympäristökeskus |
format |
Article in Journal/Newspaper |
author |
Tiwari, Ananda Kauppinen, Ari Räsänen, Pia Salonen, Jenniina Wessels, Laura Juntunen, Janne Miettinen, Ilkka T. Pitkänen, Tarja |
author_facet |
Tiwari, Ananda Kauppinen, Ari Räsänen, Pia Salonen, Jenniina Wessels, Laura Juntunen, Janne Miettinen, Ilkka T. Pitkänen, Tarja |
author_sort |
Tiwari, Ananda |
title |
Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
title_short |
Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
title_full |
Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
title_fullStr |
Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
title_full_unstemmed |
Effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and Legionella in mesocosms simulating subarctic river water |
title_sort |
effects of temperature and light exposure on the decay characteristics of fecal indicators, norovirus, and legionella in mesocosms simulating subarctic river water |
publisher |
Elsevier BV |
publishDate |
2023 |
url |
http://hdl.handle.net/10138/352618 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
160340 10.1016/j.scitotenv.2022.160340 Science of the Total Environment 0048-9697 859 http://hdl.handle.net/10138/352618 URN:NBN:fi-fe202301061727 |
op_rights |
CC BY 4.0 openAccess |
_version_ |
1774723932618752000 |