Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stabl...
Published in: | Hydrology and Earth System Sciences |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
COPERNICUS GESELLSCHAFT MBH
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/10138/350549 |
id |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/350549 |
---|---|
record_format |
openpolar |
spelling |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/350549 2024-01-07T09:45:34+01:00 Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kübert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John Institute for Atmospheric and Earth System Research (INAR) 2022-11-09T07:56:04Z 15 application/pdf http://hdl.handle.net/10138/350549 eng eng COPERNICUS GESELLSCHAFT MBH 10.5194/hess-26-3573-2022 supported through grants from the Knut and Alice Wallenberg foundation: KAW 2018.0259 (Ruth-Kristina Magh and John Marshall) and KAW 2015.0047 (John Marshall). Angelika Kübert was funded by a “Short Term Scientific Mission (STSM)” grant provided by the COST Action (CA19120) WATer isotopeS in the critical zONe (WATSON). Magh , R-K , Gralher , B , Herbstritt , B , Kübert , A , Lim , H , Lundmark , T & Marshall , J 2022 , ' Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems ' , Hydrology and Earth System Sciences , vol. 26 , no. 13 , pp. 3573-3587 . https://doi.org/10.5194/hess-26-3573-2022 ORCID: /0000-0003-3985-9261/work/122647237 85134261067 bde01b0b-a9c4-43e4-91c5-8e94411ef90c http://hdl.handle.net/10138/350549 000821944900001 cc_by openAccess info:eu-repo/semantics/openAccess 1181 Ecology evolutionary biology 1172 Environmental sciences 114 Physical sciences 1171 Geosciences PLANT WATER SOIL-WATER CRYOGENIC EXTRACTION VACUUM EXTRACTION DEUTERIUM EVAPOTRANSPIRATION DELTA-O-18 DELTA-H-2 DYNAMICS SEASON Article publishedVersion 2022 ftunivhelsihelda 2023-12-14T00:13:04Z Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from -95 parts per thousand to +1700 parts per thousand for delta H-2) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 parts per thousand to 4.4 parts per thousand for delta H-2 and 0.6 parts per thousand to 0.8 parts per thousand for delta O-18 for natural-abundance samples. Although H-2-enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for delta O-18 but observed higher variation in delta H-2 than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring ... Article in Journal/Newspaper Northern Sweden HELDA – University of Helsinki Open Repository Hydrology and Earth System Sciences 26 13 3573 3587 |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
1181 Ecology evolutionary biology 1172 Environmental sciences 114 Physical sciences 1171 Geosciences PLANT WATER SOIL-WATER CRYOGENIC EXTRACTION VACUUM EXTRACTION DEUTERIUM EVAPOTRANSPIRATION DELTA-O-18 DELTA-H-2 DYNAMICS SEASON |
spellingShingle |
1181 Ecology evolutionary biology 1172 Environmental sciences 114 Physical sciences 1171 Geosciences PLANT WATER SOIL-WATER CRYOGENIC EXTRACTION VACUUM EXTRACTION DEUTERIUM EVAPOTRANSPIRATION DELTA-O-18 DELTA-H-2 DYNAMICS SEASON Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kübert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
topic_facet |
1181 Ecology evolutionary biology 1172 Environmental sciences 114 Physical sciences 1171 Geosciences PLANT WATER SOIL-WATER CRYOGENIC EXTRACTION VACUUM EXTRACTION DEUTERIUM EVAPOTRANSPIRATION DELTA-O-18 DELTA-H-2 DYNAMICS SEASON |
description |
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from -95 parts per thousand to +1700 parts per thousand for delta H-2) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 parts per thousand to 4.4 parts per thousand for delta H-2 and 0.6 parts per thousand to 0.8 parts per thousand for delta O-18 for natural-abundance samples. Although H-2-enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for delta O-18 but observed higher variation in delta H-2 than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring ... |
author2 |
Institute for Atmospheric and Earth System Research (INAR) |
format |
Article in Journal/Newspaper |
author |
Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kübert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John |
author_facet |
Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kübert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John |
author_sort |
Magh, Ruth-Kristina |
title |
Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_short |
Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_full |
Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_fullStr |
Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_full_unstemmed |
Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_sort |
technical note : conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
publisher |
COPERNICUS GESELLSCHAFT MBH |
publishDate |
2022 |
url |
http://hdl.handle.net/10138/350549 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
10.5194/hess-26-3573-2022 supported through grants from the Knut and Alice Wallenberg foundation: KAW 2018.0259 (Ruth-Kristina Magh and John Marshall) and KAW 2015.0047 (John Marshall). Angelika Kübert was funded by a “Short Term Scientific Mission (STSM)” grant provided by the COST Action (CA19120) WATer isotopeS in the critical zONe (WATSON). Magh , R-K , Gralher , B , Herbstritt , B , Kübert , A , Lim , H , Lundmark , T & Marshall , J 2022 , ' Technical note : Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems ' , Hydrology and Earth System Sciences , vol. 26 , no. 13 , pp. 3573-3587 . https://doi.org/10.5194/hess-26-3573-2022 ORCID: /0000-0003-3985-9261/work/122647237 85134261067 bde01b0b-a9c4-43e4-91c5-8e94411ef90c http://hdl.handle.net/10138/350549 000821944900001 |
op_rights |
cc_by openAccess info:eu-repo/semantics/openAccess |
container_title |
Hydrology and Earth System Sciences |
container_volume |
26 |
container_issue |
13 |
container_start_page |
3573 |
op_container_end_page |
3587 |
_version_ |
1787427123434094592 |