In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are...
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/346111 2024-09-15T17:54:48+00:00 In-depth characterization of denitrifier communities across different soil ecosystems in the tundra Pessi, Igor S. Viitamäki, Sirja Virkkala, Anna-Maria Eronen-Rasimus, Eeva Delmont, Tom O. Marushchak, Maija E. Luoto, Miska Hultman, Jenni Suomen ympäristökeskus The Finnish Environment Institute 2022-07-11T15:17:29Z application/pdf http://hdl.handle.net/10138/346111 eng eng Springer Science and Business Media LLC Environmental Microbiome 10.1186/s40793-022-00424-2 2524-6372 1 17 http://hdl.handle.net/10138/346111 URN:NBN:fi-fe2022071151626 CC BY 4.0 openAccess arctic region denitrification soil microbiome Arctic Genome-resolved metagenomics metagenomics nitrous oxide dityppioksidi typpi nitraatit denitrifikaatio maaperä ekosysteemit (ekologia) arktinen alue tundra kasvihuonekaasut A1 Journal article (refereed), original research A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä 2022 ftunivhelsihelda 2024-07-31T23:42:49Z Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra. Results We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23. Conclusions By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities. Article in Journal/Newspaper Arktinen alue Northern Finland Tundra HELDA – University of Helsinki Open Repository |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
arctic region denitrification soil microbiome Arctic Genome-resolved metagenomics metagenomics nitrous oxide dityppioksidi typpi nitraatit denitrifikaatio maaperä ekosysteemit (ekologia) arktinen alue tundra kasvihuonekaasut |
spellingShingle |
arctic region denitrification soil microbiome Arctic Genome-resolved metagenomics metagenomics nitrous oxide dityppioksidi typpi nitraatit denitrifikaatio maaperä ekosysteemit (ekologia) arktinen alue tundra kasvihuonekaasut Pessi, Igor S. Viitamäki, Sirja Virkkala, Anna-Maria Eronen-Rasimus, Eeva Delmont, Tom O. Marushchak, Maija E. Luoto, Miska Hultman, Jenni In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
topic_facet |
arctic region denitrification soil microbiome Arctic Genome-resolved metagenomics metagenomics nitrous oxide dityppioksidi typpi nitraatit denitrifikaatio maaperä ekosysteemit (ekologia) arktinen alue tundra kasvihuonekaasut |
description |
Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra. Results We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23. Conclusions By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities. |
author2 |
Suomen ympäristökeskus The Finnish Environment Institute |
format |
Article in Journal/Newspaper |
author |
Pessi, Igor S. Viitamäki, Sirja Virkkala, Anna-Maria Eronen-Rasimus, Eeva Delmont, Tom O. Marushchak, Maija E. Luoto, Miska Hultman, Jenni |
author_facet |
Pessi, Igor S. Viitamäki, Sirja Virkkala, Anna-Maria Eronen-Rasimus, Eeva Delmont, Tom O. Marushchak, Maija E. Luoto, Miska Hultman, Jenni |
author_sort |
Pessi, Igor S. |
title |
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
title_short |
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
title_full |
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
title_fullStr |
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
title_full_unstemmed |
In-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
title_sort |
in-depth characterization of denitrifier communities across different soil ecosystems in the tundra |
publisher |
Springer Science and Business Media LLC |
publishDate |
2022 |
url |
http://hdl.handle.net/10138/346111 |
genre |
Arktinen alue Northern Finland Tundra |
genre_facet |
Arktinen alue Northern Finland Tundra |
op_relation |
Environmental Microbiome 10.1186/s40793-022-00424-2 2524-6372 1 17 http://hdl.handle.net/10138/346111 URN:NBN:fi-fe2022071151626 |
op_rights |
CC BY 4.0 openAccess |
_version_ |
1810431106078474240 |