Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway
Background Mosquito-borne viruses pose a serious threat to humans worldwide. There has been an upsurge in the number of mosquito-borne viruses in Europe, mostly belonging to the families Togaviridae, genus Alphavirus (Sindbis, Chikungunya), Flaviviridae (West Nile, Usutu, Dengue), and Peribunyavirid...
Published in: | Virology Journal |
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Format: | Article in Journal/Newspaper |
Language: | English |
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BioMed Central Ltd
2022
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Online Access: | http://hdl.handle.net/10138/345986 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/345986 |
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Open Polar |
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HELDA – University of Helsinki Open Repository |
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ftunivhelsihelda |
language |
English |
topic |
Arbovirus Blood sucking insects (BSI) Mosquito Reindeer INKV SINV Prevalence Estimated pooled prevalence IIFA Seroprevalence MOSQUITO-BORNE ARBOVIRUSES CULEX-TORRENTIUM CAUSATIVE AGENT RISK-FACTORS INFECTION SWEDEN RNA ANTIBODIES TAHYNA 11832 Microbiology and virology |
spellingShingle |
Arbovirus Blood sucking insects (BSI) Mosquito Reindeer INKV SINV Prevalence Estimated pooled prevalence IIFA Seroprevalence MOSQUITO-BORNE ARBOVIRUSES CULEX-TORRENTIUM CAUSATIVE AGENT RISK-FACTORS INFECTION SWEDEN RNA ANTIBODIES TAHYNA 11832 Microbiology and virology Shakya, Ruchika Tryland, Morten Vikse, Rose Romano, Javier Sanchez Asbakk, Kjetil Nymo, Ingebjorg H. Mehl, Reidar Evander, Magnus Ahlm, Clas Vapalahti, Olli Lwande, Olivia Wesula Putkuri, Niina Johansen, Wenche Soleng, Arnulf Edgar, Kristin S. Andreassen, Ashild K. Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
topic_facet |
Arbovirus Blood sucking insects (BSI) Mosquito Reindeer INKV SINV Prevalence Estimated pooled prevalence IIFA Seroprevalence MOSQUITO-BORNE ARBOVIRUSES CULEX-TORRENTIUM CAUSATIVE AGENT RISK-FACTORS INFECTION SWEDEN RNA ANTIBODIES TAHYNA 11832 Microbiology and virology |
description |
Background Mosquito-borne viruses pose a serious threat to humans worldwide. There has been an upsurge in the number of mosquito-borne viruses in Europe, mostly belonging to the families Togaviridae, genus Alphavirus (Sindbis, Chikungunya), Flaviviridae (West Nile, Usutu, Dengue), and Peribunyaviridae, genus Orthobunyavirus, California serogroup (Inkoo, Batai, Tahyna). The principal focus of this study was Inkoo (INKV) and Sindbis (SINV) virus circulating in Norway, Sweden, Finland, and some parts of Russia. These viruses are associated with morbidity in humans. However, there is a knowledge gap regarding reservoirs and transmission. Therefore, we aimed to determine the prevalence of INKV and SINV in blood sucking insects and seroprevalence for INKV in semi-domesticated Eurasian tundra reindeer (Rangifer tarandus tarandus) in Norway. Materials and methods In total, 213 pools containing about 25 blood sucking insects (BSI) each and 480 reindeer sera were collected in eight Norwegian reindeer summer pasture districts during 2013-2015. The pools were analysed by RT-PCR to detect INKV and by RT-real-time PCR for SINV. Reindeer sera were analysed for INKV-specific IgG by an Indirect Immunofluorescence Assay (n = 480, IIFA) and a Plaque Reduction Neutralization Test (n = 60, PRNT). Results Aedes spp. were the most dominant species among the collected BSI. Two of the pools were positive for INKV-RNA by RT-PCR and were confirmed by pyrosequencing. The overall estimated pool prevalence (EPP) of INKV in Norway was 0.04%. None of the analysed pools were positive for SINV. Overall IgG seroprevalence in reindeer was 62% positive for INKV by IIFA. Of the 60 reindeer sera- analysed by PRNT for INKV, 80% were confirmed positive, and there was no cross-reactivity with the closely related Tahyna virus (TAHV) and Snowshoe hare virus (SSHV). Conclusion The occurrence and prevalence of INKV in BSI and the high seroprevalence against the virus among semi-domesticated reindeer in Norway indicate that further studies are required for ... |
author2 |
University of Helsinki Department of Virology Veterinary Biosciences Veterinary Microbiology and Epidemiology Helsinki One Health (HOH) Viral Zoonosis Research Unit HUSLAB |
format |
Article in Journal/Newspaper |
author |
Shakya, Ruchika Tryland, Morten Vikse, Rose Romano, Javier Sanchez Asbakk, Kjetil Nymo, Ingebjorg H. Mehl, Reidar Evander, Magnus Ahlm, Clas Vapalahti, Olli Lwande, Olivia Wesula Putkuri, Niina Johansen, Wenche Soleng, Arnulf Edgar, Kristin S. Andreassen, Ashild K. |
author_facet |
Shakya, Ruchika Tryland, Morten Vikse, Rose Romano, Javier Sanchez Asbakk, Kjetil Nymo, Ingebjorg H. Mehl, Reidar Evander, Magnus Ahlm, Clas Vapalahti, Olli Lwande, Olivia Wesula Putkuri, Niina Johansen, Wenche Soleng, Arnulf Edgar, Kristin S. Andreassen, Ashild K. |
author_sort |
Shakya, Ruchika |
title |
Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
title_short |
Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
title_full |
Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
title_fullStr |
Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
title_full_unstemmed |
Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway |
title_sort |
inkoo and sindbis viruses in blood sucking insects, and a serological study for inkoo virus in semi-domesticated eurasian tundra reindeer in norway |
publisher |
BioMed Central Ltd |
publishDate |
2022 |
url |
http://hdl.handle.net/10138/345986 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rangifer tarandus Tundra |
genre_facet |
Rangifer tarandus Tundra |
op_relation |
10.1186/s12985-022-01815-0 This study was funded by the Norwegian Ministry of Health and Care Services, Barents region project B1710 "Emerging infections. Capacity building on vector-borne infections in the Barents region, Norway and Russia". Sample collecting was funded by grants from the Norwegian Reindeer Development Fund (RUF) and by the Fram Center flagship "Klimaeffekter pa okosystemer, landskap, lokalsamfunn og urfolk", grant nr. 362256. Sample analysis was funded by grants from Insamlingsstiftelsen, Medical faculty, Umea University, Sweden, and through a regional agreement between Umea University and Vasterbotten County Council (ALF). Shakya , R , Tryland , M , Vikse , R , Romano , J S , Asbakk , K , Nymo , I H , Mehl , R , Evander , M , Ahlm , C , Vapalahti , O , Lwande , O W , Putkuri , N , Johansen , W , Soleng , A , Edgar , K S & Andreassen , A K 2022 , ' Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway ' , Virology Journal , vol. 19 , no. 1 , 99 . https://doi.org/10.1186/s12985-022-01815-0 ORCID: /0000-0003-2270-6824/work/115592892 d02e2cb4-2de1-43fa-a16f-339cb3549d81 http://hdl.handle.net/10138/345986 000805782600003 |
op_rights |
cc_by openAccess info:eu-repo/semantics/openAccess |
container_title |
Virology Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1787427914488217600 |
spelling |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/345986 2024-01-07T09:46:11+01:00 Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway Shakya, Ruchika Tryland, Morten Vikse, Rose Romano, Javier Sanchez Asbakk, Kjetil Nymo, Ingebjorg H. Mehl, Reidar Evander, Magnus Ahlm, Clas Vapalahti, Olli Lwande, Olivia Wesula Putkuri, Niina Johansen, Wenche Soleng, Arnulf Edgar, Kristin S. Andreassen, Ashild K. University of Helsinki Department of Virology Veterinary Biosciences Veterinary Microbiology and Epidemiology Helsinki One Health (HOH) Viral Zoonosis Research Unit HUSLAB 2022-07-07T13:04:01Z 11 application/pdf http://hdl.handle.net/10138/345986 eng eng BioMed Central Ltd 10.1186/s12985-022-01815-0 This study was funded by the Norwegian Ministry of Health and Care Services, Barents region project B1710 "Emerging infections. Capacity building on vector-borne infections in the Barents region, Norway and Russia". Sample collecting was funded by grants from the Norwegian Reindeer Development Fund (RUF) and by the Fram Center flagship "Klimaeffekter pa okosystemer, landskap, lokalsamfunn og urfolk", grant nr. 362256. Sample analysis was funded by grants from Insamlingsstiftelsen, Medical faculty, Umea University, Sweden, and through a regional agreement between Umea University and Vasterbotten County Council (ALF). Shakya , R , Tryland , M , Vikse , R , Romano , J S , Asbakk , K , Nymo , I H , Mehl , R , Evander , M , Ahlm , C , Vapalahti , O , Lwande , O W , Putkuri , N , Johansen , W , Soleng , A , Edgar , K S & Andreassen , A K 2022 , ' Inkoo and Sindbis viruses in blood sucking insects, and a serological study for Inkoo virus in semi-domesticated Eurasian tundra reindeer in Norway ' , Virology Journal , vol. 19 , no. 1 , 99 . https://doi.org/10.1186/s12985-022-01815-0 ORCID: /0000-0003-2270-6824/work/115592892 d02e2cb4-2de1-43fa-a16f-339cb3549d81 http://hdl.handle.net/10138/345986 000805782600003 cc_by openAccess info:eu-repo/semantics/openAccess Arbovirus Blood sucking insects (BSI) Mosquito Reindeer INKV SINV Prevalence Estimated pooled prevalence IIFA Seroprevalence MOSQUITO-BORNE ARBOVIRUSES CULEX-TORRENTIUM CAUSATIVE AGENT RISK-FACTORS INFECTION SWEDEN RNA ANTIBODIES TAHYNA 11832 Microbiology and virology Article publishedVersion 2022 ftunivhelsihelda 2023-12-14T00:07:30Z Background Mosquito-borne viruses pose a serious threat to humans worldwide. There has been an upsurge in the number of mosquito-borne viruses in Europe, mostly belonging to the families Togaviridae, genus Alphavirus (Sindbis, Chikungunya), Flaviviridae (West Nile, Usutu, Dengue), and Peribunyaviridae, genus Orthobunyavirus, California serogroup (Inkoo, Batai, Tahyna). The principal focus of this study was Inkoo (INKV) and Sindbis (SINV) virus circulating in Norway, Sweden, Finland, and some parts of Russia. These viruses are associated with morbidity in humans. However, there is a knowledge gap regarding reservoirs and transmission. Therefore, we aimed to determine the prevalence of INKV and SINV in blood sucking insects and seroprevalence for INKV in semi-domesticated Eurasian tundra reindeer (Rangifer tarandus tarandus) in Norway. Materials and methods In total, 213 pools containing about 25 blood sucking insects (BSI) each and 480 reindeer sera were collected in eight Norwegian reindeer summer pasture districts during 2013-2015. The pools were analysed by RT-PCR to detect INKV and by RT-real-time PCR for SINV. Reindeer sera were analysed for INKV-specific IgG by an Indirect Immunofluorescence Assay (n = 480, IIFA) and a Plaque Reduction Neutralization Test (n = 60, PRNT). Results Aedes spp. were the most dominant species among the collected BSI. Two of the pools were positive for INKV-RNA by RT-PCR and were confirmed by pyrosequencing. The overall estimated pool prevalence (EPP) of INKV in Norway was 0.04%. None of the analysed pools were positive for SINV. Overall IgG seroprevalence in reindeer was 62% positive for INKV by IIFA. Of the 60 reindeer sera- analysed by PRNT for INKV, 80% were confirmed positive, and there was no cross-reactivity with the closely related Tahyna virus (TAHV) and Snowshoe hare virus (SSHV). Conclusion The occurrence and prevalence of INKV in BSI and the high seroprevalence against the virus among semi-domesticated reindeer in Norway indicate that further studies are required for ... Article in Journal/Newspaper Rangifer tarandus Tundra HELDA – University of Helsinki Open Repository Norway Virology Journal 19 1 |