Sanguiin H-6 Fractionated from Cloudberry (Rubus chamaemorus) Seeds Can Prevent the Methicillin-Resistant Staphylococcus aureus Biofilm Development during Wound Infection

• Published in: Antibiotics
• Main Authors: Aguilera-Correa, John Jairo, Fernandez-Lopez, Sara, Cunas-Figueroa, Iskra Dennisse, Perez-Rial, Sandra, Alakomi, Hanna-Leena, Nohynek, Liisa, Oksman-Caldentey, Kirsi-Marja, Salminen, Juha-Pekka, Esteban, Jaime, Cuadros, Juan, Puupponen-Pimia, Riitta, Perez-Tanoira, Ramon, Kinnari, Teemu J.
• Other Authors: HUS Head and Neck Center, Korva-, nenä- ja kurkkutautien klinikka, Clinicum
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI 2022
• Subjects: methicillin-resistant S; aureus; biofilm; cloudberry; Rubus; ellagitannin; ELLAGITANNINS; FRUITS; ANTIBIOTICS; ANTIOXIDANT; BIOACTIVITY; EXTRACTS; MRSA; 3121 General medicine; internal medicine and other clinical medicine; 317 Pharmacy; Rubus chamaemorus
• Online Access: http://hdl.handle.net/10138/338949

Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity. Peer reviewed