Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
Highlights • Amplicon Sequence Variants (ASVs) are a promising unit for diatom nutrient indices. • Diatom indices based on morphology, ASVs and traits were related to TotP concentrations. • Correlations of indices to TotP was best for ASVs, followed by morphology and traits. We developed and compare...
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Language: | English |
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Elsevier
2021
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Online Access: | http://hdl.handle.net/10138/336722 https://doi.org/10.1016/j.ecolind.2021.108088 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/336722 2023-08-20T04:06:25+02:00 Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared Kahlert, Maria Bailet, Bonnie Chonova, Teofana Karjalainen, Satu Maaria Schneider, Susanne C. Tapolczai, Kálmán 2021-11-25T10:27:23Z application/pdf http://hdl.handle.net/10138/336722 https://doi.org/10.1016/j.ecolind.2021.108088 eng eng Elsevier Ecological Indicators 130: 108088 1470-160X Maria Kahlert, Bonnie Bailet, Teofana Chonova, Satu Maaria Karjalainen, Susanne C. Schneider, Kálmán Tapolczai. Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared. Ecological Indicators 130 (2021), 108088, ISSN 1470-160X. https://doi.org/10.1016/j.ecolind.2021.108088 https://doi.org/10.1016/j.ecolind.2021.108088 Suomen ympäristökeskus http://hdl.handle.net/10138/336722 CC BY 4.0 openAccess Amplicon Sequence Variants environmental assessment index indicator value eutrophication metabarcoding DNA metabarcoding evaluation rivers diatoms state of the nature water quality fresh water indicators indices Nordic countries Fennoscandia arviointi piilevät järvet vedenlaatu joet ympäristön tila makea vesi indikaattorit indeksit rehevöityminen DNA-viivakoodit Pohjoismaat Fennoskandia Article 2021 ftunivhelsihelda https://doi.org/10.1016/j.ecolind.2021.108088 2023-07-28T06:25:13Z Highlights • Amplicon Sequence Variants (ASVs) are a promising unit for diatom nutrient indices. • Diatom indices based on morphology, ASVs and traits were related to TotP concentrations. • Correlations of indices to TotP was best for ASVs, followed by morphology and traits. We developed and compared the performance of freshwater benthic diatom indices calculated from (i) traditional morphological species identification, (ii) Amplicon Sequence Variants (ASVs) obtained via DNA metabarcoding, and (iii) morphological traits to indicate eutrophication in rivers and lakes in Fennoscandia. Based on the results, we provided recommendations for the future routine use of diatom bioassessment tools in environmental monitoring and assessment. Our results show that ASVs are the most promising candidates to be used in environmental assessment. Indices based on ASVs correlated better with TotP concentrations than morphological taxa data, whereas the trait indices correlated least. We could see by studying the taxonomic assignments of the ASVs that traditional morphotaxa were divided up into several ASVs with different ecological profiles, which explained part of the better index performance and also encourages further studies on diatom diversity and ecological preferences. In general, ASV- and morphotaxon-specific optima differed slightly between streams and lakes, but were significantly correlated with each other. This means that it should be possible to develop a common index that is applicable in both streams and lakes, but boundary values with respect to TotP might need to be set separately for them. More knowledge on diatom traits is required to enable their use for environmental assessment. Article in Journal/Newspaper Fennoscandia Fennoscandian Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto Ecological Indicators 130 108088 |
institution |
Open Polar |
collection |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
Amplicon Sequence Variants environmental assessment index indicator value eutrophication metabarcoding DNA metabarcoding evaluation rivers diatoms state of the nature water quality fresh water indicators indices Nordic countries Fennoscandia arviointi piilevät järvet vedenlaatu joet ympäristön tila makea vesi indikaattorit indeksit rehevöityminen DNA-viivakoodit Pohjoismaat Fennoskandia |
spellingShingle |
Amplicon Sequence Variants environmental assessment index indicator value eutrophication metabarcoding DNA metabarcoding evaluation rivers diatoms state of the nature water quality fresh water indicators indices Nordic countries Fennoscandia arviointi piilevät järvet vedenlaatu joet ympäristön tila makea vesi indikaattorit indeksit rehevöityminen DNA-viivakoodit Pohjoismaat Fennoskandia Kahlert, Maria Bailet, Bonnie Chonova, Teofana Karjalainen, Satu Maaria Schneider, Susanne C. Tapolczai, Kálmán Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
topic_facet |
Amplicon Sequence Variants environmental assessment index indicator value eutrophication metabarcoding DNA metabarcoding evaluation rivers diatoms state of the nature water quality fresh water indicators indices Nordic countries Fennoscandia arviointi piilevät järvet vedenlaatu joet ympäristön tila makea vesi indikaattorit indeksit rehevöityminen DNA-viivakoodit Pohjoismaat Fennoskandia |
description |
Highlights • Amplicon Sequence Variants (ASVs) are a promising unit for diatom nutrient indices. • Diatom indices based on morphology, ASVs and traits were related to TotP concentrations. • Correlations of indices to TotP was best for ASVs, followed by morphology and traits. We developed and compared the performance of freshwater benthic diatom indices calculated from (i) traditional morphological species identification, (ii) Amplicon Sequence Variants (ASVs) obtained via DNA metabarcoding, and (iii) morphological traits to indicate eutrophication in rivers and lakes in Fennoscandia. Based on the results, we provided recommendations for the future routine use of diatom bioassessment tools in environmental monitoring and assessment. Our results show that ASVs are the most promising candidates to be used in environmental assessment. Indices based on ASVs correlated better with TotP concentrations than morphological taxa data, whereas the trait indices correlated least. We could see by studying the taxonomic assignments of the ASVs that traditional morphotaxa were divided up into several ASVs with different ecological profiles, which explained part of the better index performance and also encourages further studies on diatom diversity and ecological preferences. In general, ASV- and morphotaxon-specific optima differed slightly between streams and lakes, but were significantly correlated with each other. This means that it should be possible to develop a common index that is applicable in both streams and lakes, but boundary values with respect to TotP might need to be set separately for them. More knowledge on diatom traits is required to enable their use for environmental assessment. |
format |
Article in Journal/Newspaper |
author |
Kahlert, Maria Bailet, Bonnie Chonova, Teofana Karjalainen, Satu Maaria Schneider, Susanne C. Tapolczai, Kálmán |
author_facet |
Kahlert, Maria Bailet, Bonnie Chonova, Teofana Karjalainen, Satu Maaria Schneider, Susanne C. Tapolczai, Kálmán |
author_sort |
Kahlert, Maria |
title |
Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
title_short |
Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
title_full |
Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
title_fullStr |
Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
title_full_unstemmed |
Same same, but different : The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared |
title_sort |
same same, but different : the response of diatoms to environmental gradients in fennoscandian streams and lakes – barcodes, traits and microscope data compared |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://hdl.handle.net/10138/336722 https://doi.org/10.1016/j.ecolind.2021.108088 |
genre |
Fennoscandia Fennoscandian |
genre_facet |
Fennoscandia Fennoscandian |
op_relation |
Ecological Indicators 130: 108088 1470-160X Maria Kahlert, Bonnie Bailet, Teofana Chonova, Satu Maaria Karjalainen, Susanne C. Schneider, Kálmán Tapolczai. Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared. Ecological Indicators 130 (2021), 108088, ISSN 1470-160X. https://doi.org/10.1016/j.ecolind.2021.108088 https://doi.org/10.1016/j.ecolind.2021.108088 Suomen ympäristökeskus http://hdl.handle.net/10138/336722 |
op_rights |
CC BY 4.0 openAccess |
op_doi |
https://doi.org/10.1016/j.ecolind.2021.108088 |
container_title |
Ecological Indicators |
container_volume |
130 |
container_start_page |
108088 |
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1774717450973085696 |