Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe
The picornavirus named 'Ljungan virus' (LV, species Parechovirus B) has been detected in a dozen small mammal species from across Europe, but detailed information on its genetic diversity and host specificity is lacking. Here, we analyze the evolutionary relationships of LV variants circul...
Published in: | Viruses |
---|---|
Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2021
|
Subjects: | |
Online Access: | http://hdl.handle.net/10138/334316 |
id |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/334316 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
Picornaviridae Parechovirus B Ljungan virus isolates small mammals rodent-borne virus zoonosis bank vole VOLES MYODES-GLAREOLUS HUMAN PARECHOVIRUSES LYMPHOCYTIC CHORIOMENINGITIS MOLECULAR CHARACTERIZATION MAXIMUM-LIKELIHOOD SEROLOGICAL SURVEY MITOCHONDRIAL-DNA CLINICAL-SAMPLES PUUMALA VIRUSES PUBLIC-HEALTH 3111 Biomedicine 11832 Microbiology and virology |
spellingShingle |
Picornaviridae Parechovirus B Ljungan virus isolates small mammals rodent-borne virus zoonosis bank vole VOLES MYODES-GLAREOLUS HUMAN PARECHOVIRUSES LYMPHOCYTIC CHORIOMENINGITIS MOLECULAR CHARACTERIZATION MAXIMUM-LIKELIHOOD SEROLOGICAL SURVEY MITOCHONDRIAL-DNA CLINICAL-SAMPLES PUUMALA VIRUSES PUBLIC-HEALTH 3111 Biomedicine 11832 Microbiology and virology Rossi, Chiara Zadra, Nicola Fevola, Cristina Ecke, Frauke Hornfeldt, Birger Kallies, Rene Kazimirova, Maria Magnusson, Magnus Olsson, Gert E. Ulrich, Rainer G. Jaaskelainen, Anne J. Henttonen, Heikki Hauffe, Heidi C. Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
topic_facet |
Picornaviridae Parechovirus B Ljungan virus isolates small mammals rodent-borne virus zoonosis bank vole VOLES MYODES-GLAREOLUS HUMAN PARECHOVIRUSES LYMPHOCYTIC CHORIOMENINGITIS MOLECULAR CHARACTERIZATION MAXIMUM-LIKELIHOOD SEROLOGICAL SURVEY MITOCHONDRIAL-DNA CLINICAL-SAMPLES PUUMALA VIRUSES PUBLIC-HEALTH 3111 Biomedicine 11832 Microbiology and virology |
description |
The picornavirus named 'Ljungan virus' (LV, species Parechovirus B) has been detected in a dozen small mammal species from across Europe, but detailed information on its genetic diversity and host specificity is lacking. Here, we analyze the evolutionary relationships of LV variants circulating in free-living mammal populations by comparing the phylogenetics of the VP1 region (encoding the capsid protein and associated with LV serotype) and the 3D(pol) region (encoding the RNA polymerase) from 24 LV RNA-positive animals and a fragment of the 5 ' untranslated region (UTR) sequence (used for defining strains) in sympatric small mammals. We define three new VP1 genotypes: two in bank voles (Myodes glareolus) (genotype 8 from Finland, Sweden, France, and Italy, and genotype 9 from France and Italy) and one in field voles (Microtus arvalis) (genotype 7 from Finland). There are several other indications that LV variants are host-specific, at least in parts of their range. Our results suggest that LV evolution is rapid, ongoing and affected by genetic drift, purifying selection, spillover and host evolutionary history. Although recent studies suggest that LV does not have zoonotic potential, its widespread geographical and host distribution in natural populations of well-characterized small mammals could make it useful as a model for studying RNA virus evolution and transmission. Peer reviewed |
author2 |
Department of Virology Faculty of Medicine University of Helsinki |
format |
Article in Journal/Newspaper |
author |
Rossi, Chiara Zadra, Nicola Fevola, Cristina Ecke, Frauke Hornfeldt, Birger Kallies, Rene Kazimirova, Maria Magnusson, Magnus Olsson, Gert E. Ulrich, Rainer G. Jaaskelainen, Anne J. Henttonen, Heikki Hauffe, Heidi C. |
author_facet |
Rossi, Chiara Zadra, Nicola Fevola, Cristina Ecke, Frauke Hornfeldt, Birger Kallies, Rene Kazimirova, Maria Magnusson, Magnus Olsson, Gert E. Ulrich, Rainer G. Jaaskelainen, Anne J. Henttonen, Heikki Hauffe, Heidi C. |
author_sort |
Rossi, Chiara |
title |
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
title_short |
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
title_full |
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
title_fullStr |
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
title_full_unstemmed |
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe |
title_sort |
evolutionary relationships of ljungan virus variants circulating in multi-host systems across europe |
publisher |
MDPI |
publishDate |
2021 |
url |
http://hdl.handle.net/10138/334316 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
10.3390/v13071317 Laboratory work was funded by the European Union grant FP7?261504 EDENext?Biology and control of vector-borne infections in Europe to A.R., H.H., R.G.U., and H.C.H., by Sigrid Jus?lius Foundation to C.F., and the Fondazione E. Mach to C.R., C.F., H.C.H. Sample collection in Sweden was financed by grants from the Swedish Environmental Protection Agency (by the National Environmental Monitoring Programme for small rodents and Alvins fond) to B.H.; the Stiftelsen Oscar och Lili Lamms minne to B.H. and F.E.; VINNOVA?Swedish Governmental Agency .? for Innovation Systems (Verket Fo Innovations system) (P32060?1) to F.E.; the Swedish Research Council Formas (221-2012-1562) to F.E., B.H., and G.E.O.; the National Environmental and Wildlife Monitoring and Assessment program (FoMA, www.slu.se/en/environment) to G.E.O.; and the Helge Ax:son Johnsons Stiftelse to M.M. The collection of rodents in Germany was commissioned and funded by the Federal Environment Agency (UBA) within the Environment Research Plan of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU). Acknowledgments: We thank Elena Buzan, Nathalie Charbonnel, Nicola Ferrari, Miriam Maas, Jaroslav Pi?lek, Claudia Romeo and Michal Stanko for providing samples. We also thank Roberto Celva for help with the Figure 1. Rossi , C , Zadra , N , Fevola , C , Ecke , F , Hornfeldt , B , Kallies , R , Kazimirova , M , Magnusson , M , Olsson , G E , Ulrich , R G , Jaaskelainen , A J , Henttonen , H & Hauffe , H C 2021 , ' Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe ' , Viruses (Basel) , vol. 13 , no. 7 , 1317 . https://doi.org/10.3390/v13071317 2a717831-e77a-4632-8940-0abace487298 http://hdl.handle.net/10138/334316 000677027800001 |
op_rights |
cc_by openAccess info:eu-repo/semantics/openAccess |
container_title |
Viruses |
container_volume |
13 |
container_issue |
7 |
container_start_page |
1317 |
_version_ |
1787426245343969280 |
spelling |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/334316 2024-01-07T09:44:49+01:00 Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe Rossi, Chiara Zadra, Nicola Fevola, Cristina Ecke, Frauke Hornfeldt, Birger Kallies, Rene Kazimirova, Maria Magnusson, Magnus Olsson, Gert E. Ulrich, Rainer G. Jaaskelainen, Anne J. Henttonen, Heikki Hauffe, Heidi C. Department of Virology Faculty of Medicine University of Helsinki 2021-09-13T12:05:01Z 18 application/pdf http://hdl.handle.net/10138/334316 eng eng MDPI 10.3390/v13071317 Laboratory work was funded by the European Union grant FP7?261504 EDENext?Biology and control of vector-borne infections in Europe to A.R., H.H., R.G.U., and H.C.H., by Sigrid Jus?lius Foundation to C.F., and the Fondazione E. Mach to C.R., C.F., H.C.H. Sample collection in Sweden was financed by grants from the Swedish Environmental Protection Agency (by the National Environmental Monitoring Programme for small rodents and Alvins fond) to B.H.; the Stiftelsen Oscar och Lili Lamms minne to B.H. and F.E.; VINNOVA?Swedish Governmental Agency .? for Innovation Systems (Verket Fo Innovations system) (P32060?1) to F.E.; the Swedish Research Council Formas (221-2012-1562) to F.E., B.H., and G.E.O.; the National Environmental and Wildlife Monitoring and Assessment program (FoMA, www.slu.se/en/environment) to G.E.O.; and the Helge Ax:son Johnsons Stiftelse to M.M. The collection of rodents in Germany was commissioned and funded by the Federal Environment Agency (UBA) within the Environment Research Plan of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU). Acknowledgments: We thank Elena Buzan, Nathalie Charbonnel, Nicola Ferrari, Miriam Maas, Jaroslav Pi?lek, Claudia Romeo and Michal Stanko for providing samples. We also thank Roberto Celva for help with the Figure 1. Rossi , C , Zadra , N , Fevola , C , Ecke , F , Hornfeldt , B , Kallies , R , Kazimirova , M , Magnusson , M , Olsson , G E , Ulrich , R G , Jaaskelainen , A J , Henttonen , H & Hauffe , H C 2021 , ' Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe ' , Viruses (Basel) , vol. 13 , no. 7 , 1317 . https://doi.org/10.3390/v13071317 2a717831-e77a-4632-8940-0abace487298 http://hdl.handle.net/10138/334316 000677027800001 cc_by openAccess info:eu-repo/semantics/openAccess Picornaviridae Parechovirus B Ljungan virus isolates small mammals rodent-borne virus zoonosis bank vole VOLES MYODES-GLAREOLUS HUMAN PARECHOVIRUSES LYMPHOCYTIC CHORIOMENINGITIS MOLECULAR CHARACTERIZATION MAXIMUM-LIKELIHOOD SEROLOGICAL SURVEY MITOCHONDRIAL-DNA CLINICAL-SAMPLES PUUMALA VIRUSES PUBLIC-HEALTH 3111 Biomedicine 11832 Microbiology and virology Article publishedVersion 2021 ftunivhelsihelda 2023-12-14T00:06:20Z The picornavirus named 'Ljungan virus' (LV, species Parechovirus B) has been detected in a dozen small mammal species from across Europe, but detailed information on its genetic diversity and host specificity is lacking. Here, we analyze the evolutionary relationships of LV variants circulating in free-living mammal populations by comparing the phylogenetics of the VP1 region (encoding the capsid protein and associated with LV serotype) and the 3D(pol) region (encoding the RNA polymerase) from 24 LV RNA-positive animals and a fragment of the 5 ' untranslated region (UTR) sequence (used for defining strains) in sympatric small mammals. We define three new VP1 genotypes: two in bank voles (Myodes glareolus) (genotype 8 from Finland, Sweden, France, and Italy, and genotype 9 from France and Italy) and one in field voles (Microtus arvalis) (genotype 7 from Finland). There are several other indications that LV variants are host-specific, at least in parts of their range. Our results suggest that LV evolution is rapid, ongoing and affected by genetic drift, purifying selection, spillover and host evolutionary history. Although recent studies suggest that LV does not have zoonotic potential, its widespread geographical and host distribution in natural populations of well-characterized small mammals could make it useful as a model for studying RNA virus evolution and transmission. Peer reviewed Article in Journal/Newspaper Microtus arvalis HELDA – University of Helsinki Open Repository Viruses 13 7 1317 |