Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton
The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytome...
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Suomen ympäristökeskus
2021
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/334081 2023-08-20T04:05:40+02:00 Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton Salmi, Pauliina Mäki, Anita Mikkonen, Anu Pupponen, Veli-Mikko Vuorio, Kristiina Tiirola, Marja 2021-09-07T15:32:55Z 17-27 application/pdf http://hdl.handle.net/10138/334081 eng eng Suomen ympäristökeskus Boreal Environment Research 1239-6095 26 Salmi P., Mäki A., Mikkonen A., Pupponen V-M., Vuorio K. & Tiiola M. 2021: Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton. Boreal Env. Res. 26: 17–27. Suomen ympäristökeskus http://hdl.handle.net/10138/334081 CC BY 4.0 openAccess microscopy plankton microalgae calculation models cyanobacteria blue-green algae fluorescence microscopy biomass biomass (ecology) mikroskopia mikrolevät laskentamallit syanobakteerit fluoresenssimikroskopia biomassa biomassa (ekologia) Article 2021 ftunivhelsihelda 2023-07-28T06:25:03Z The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytometry in assessing the composition, abundance and cell sizes of autofluorescent picophytoplankton in epilimnia of 46 Finnish lakes. Phycocyaninrich picocyanobacteria were the most dominant. The two methods yielded comparable total picophytoplankton abundances, but the determination of cell sizes, and thus total biomasses, were on average an order of magnitude higher in the microscopy results. However, flow cytometry yielded higher cell sizes when applied on small-celled cultured algae. Our study demonstrated that both epifluorescence microscopy and flow cytometry are useful methods in assessing abundances of phycocyanin-rich and phycoerythrin-rich picocyanobacteria and eukaryotic picophytoplankton in lakes. However, accurate determination of cell size and biomass remain challenges for microscopy and especially for flow cytometry. Article in Journal/Newspaper Boreal Environment Research Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
institution |
Open Polar |
collection |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
microscopy plankton microalgae calculation models cyanobacteria blue-green algae fluorescence microscopy biomass biomass (ecology) mikroskopia mikrolevät laskentamallit syanobakteerit fluoresenssimikroskopia biomassa biomassa (ekologia) |
spellingShingle |
microscopy plankton microalgae calculation models cyanobacteria blue-green algae fluorescence microscopy biomass biomass (ecology) mikroskopia mikrolevät laskentamallit syanobakteerit fluoresenssimikroskopia biomassa biomassa (ekologia) Salmi, Pauliina Mäki, Anita Mikkonen, Anu Pupponen, Veli-Mikko Vuorio, Kristiina Tiirola, Marja Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
topic_facet |
microscopy plankton microalgae calculation models cyanobacteria blue-green algae fluorescence microscopy biomass biomass (ecology) mikroskopia mikrolevät laskentamallit syanobakteerit fluoresenssimikroskopia biomassa biomassa (ekologia) |
description |
The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytometry in assessing the composition, abundance and cell sizes of autofluorescent picophytoplankton in epilimnia of 46 Finnish lakes. Phycocyaninrich picocyanobacteria were the most dominant. The two methods yielded comparable total picophytoplankton abundances, but the determination of cell sizes, and thus total biomasses, were on average an order of magnitude higher in the microscopy results. However, flow cytometry yielded higher cell sizes when applied on small-celled cultured algae. Our study demonstrated that both epifluorescence microscopy and flow cytometry are useful methods in assessing abundances of phycocyanin-rich and phycoerythrin-rich picocyanobacteria and eukaryotic picophytoplankton in lakes. However, accurate determination of cell size and biomass remain challenges for microscopy and especially for flow cytometry. |
format |
Article in Journal/Newspaper |
author |
Salmi, Pauliina Mäki, Anita Mikkonen, Anu Pupponen, Veli-Mikko Vuorio, Kristiina Tiirola, Marja |
author_facet |
Salmi, Pauliina Mäki, Anita Mikkonen, Anu Pupponen, Veli-Mikko Vuorio, Kristiina Tiirola, Marja |
author_sort |
Salmi, Pauliina |
title |
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
title_short |
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
title_full |
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
title_fullStr |
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
title_full_unstemmed |
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
title_sort |
comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton |
publisher |
Suomen ympäristökeskus |
publishDate |
2021 |
url |
http://hdl.handle.net/10138/334081 |
genre |
Boreal Environment Research |
genre_facet |
Boreal Environment Research |
op_relation |
Boreal Environment Research 1239-6095 26 Salmi P., Mäki A., Mikkonen A., Pupponen V-M., Vuorio K. & Tiiola M. 2021: Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton. Boreal Env. Res. 26: 17–27. Suomen ympäristökeskus http://hdl.handle.net/10138/334081 |
op_rights |
CC BY 4.0 openAccess |
_version_ |
1774716341796732928 |