Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated P...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , , , , , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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American Society for Microbiology
2021
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Online Access: | http://hdl.handle.net/10138/333562 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/333562 |
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Open Polar |
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HELDA – University of Helsinki Open Repository |
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ftunivhelsihelda |
language |
English |
topic |
11832 Microbiology and virology Halomonas Paracoccus poly-3-hydroxyalkanoic acid PHA SCL-PHA MCL-PHA copolymer sea-ice bacteria marine bacteria transcriptomics genomics CHAIN-LENGTH CONSTITUENTS POLYHYDROXYALKANOIC ACID 3-HYDROXYBUTYRIC ACID READ ALIGNMENT SYNTHASE BIOSYNTHESIS PATHWAY GENES POLY(3-HYDROXYALKANOATES) POLYHYDROXYBUTYRATE |
spellingShingle |
11832 Microbiology and virology Halomonas Paracoccus poly-3-hydroxyalkanoic acid PHA SCL-PHA MCL-PHA copolymer sea-ice bacteria marine bacteria transcriptomics genomics CHAIN-LENGTH CONSTITUENTS POLYHYDROXYALKANOIC ACID 3-HYDROXYBUTYRIC ACID READ ALIGNMENT SYNTHASE BIOSYNTHESIS PATHWAY GENES POLY(3-HYDROXYALKANOATES) POLYHYDROXYBUTYRATE Eronen-Rasimus, Eeva Liisa Hultman, Jenni Hai, T Pessi, Igor S Collins, Eric Wright, S Laine, Pia Viitamäki, Sirja Lyra, Christina Thomas, David Neville Golyshin, Peter Luhtanen, Anne-Mari Kuosa, Harri Kaartokallio, Hermanni Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
topic_facet |
11832 Microbiology and virology Halomonas Paracoccus poly-3-hydroxyalkanoic acid PHA SCL-PHA MCL-PHA copolymer sea-ice bacteria marine bacteria transcriptomics genomics CHAIN-LENGTH CONSTITUENTS POLYHYDROXYALKANOIC ACID 3-HYDROXYBUTYRIC ACID READ ALIGNMENT SYNTHASE BIOSYNTHESIS PATHWAY GENES POLY(3-HYDROXYALKANOATES) POLYHYDROXYBUTYRATE |
description |
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4 degrees C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. Peer reviewed |
author2 |
Department of Microbiology Institute of Biotechnology Arctic Microbial Ecology Fungal Genetics and Biotechnology Department of Food and Nutrition Ecosystems and Environment Research Programme Molecular Principles of Viruses General Microbiology |
format |
Article in Journal/Newspaper |
author |
Eronen-Rasimus, Eeva Liisa Hultman, Jenni Hai, T Pessi, Igor S Collins, Eric Wright, S Laine, Pia Viitamäki, Sirja Lyra, Christina Thomas, David Neville Golyshin, Peter Luhtanen, Anne-Mari Kuosa, Harri Kaartokallio, Hermanni |
author_facet |
Eronen-Rasimus, Eeva Liisa Hultman, Jenni Hai, T Pessi, Igor S Collins, Eric Wright, S Laine, Pia Viitamäki, Sirja Lyra, Christina Thomas, David Neville Golyshin, Peter Luhtanen, Anne-Mari Kuosa, Harri Kaartokallio, Hermanni |
author_sort |
Eronen-Rasimus, Eeva Liisa |
title |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_short |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_fullStr |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full_unstemmed |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_sort |
sea-ice bacteria halomonas sp. strain 363 and paracoccus sp. strain 392 produce multiple types of poly-3-hydroxyalkaonoic acid (pha) storage polymers at low temperature |
publisher |
American Society for Microbiology |
publishDate |
2021 |
url |
http://hdl.handle.net/10138/333562 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Sea ice Southern Ocean |
genre_facet |
Sea ice Southern Ocean |
op_relation |
10.1128/AEM.00929-21 Eronen-Rasimus , E L , Hultman , J , Hai , T , Pessi , I S , Collins , E , Wright , S , Laine , P , Viitamäki , S , Lyra , C , Thomas , D N , Golyshin , P , Luhtanen , A-M , Kuosa , H & Kaartokallio , H 2021 , ' Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature ' , Applied and Environmental Microbiology , vol. 87 , no. 17 , ARTN e00929-21 . https://doi.org/10.1128/AEM.00929-21 ORCID: /0000-0001-8832-5907/work/98975859 ORCID: /0000-0002-2427-6162/work/98976274 ORCID: /0000-0001-5926-7496/work/157598526 http://hdl.handle.net/10138/333562 aec232c9-8028-4432-99b9-171453f24133 000693759300020 |
op_rights |
cc_by info:eu-repo/semantics/openAccess openAccess |
container_title |
Applied and Environmental Microbiology |
container_volume |
87 |
container_issue |
17 |
_version_ |
1798854377739386880 |
spelling |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/333562 2024-05-12T08:10:50+00:00 Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature Eronen-Rasimus, Eeva Liisa Hultman, Jenni Hai, T Pessi, Igor S Collins, Eric Wright, S Laine, Pia Viitamäki, Sirja Lyra, Christina Thomas, David Neville Golyshin, Peter Luhtanen, Anne-Mari Kuosa, Harri Kaartokallio, Hermanni Department of Microbiology Institute of Biotechnology Arctic Microbial Ecology Fungal Genetics and Biotechnology Department of Food and Nutrition Ecosystems and Environment Research Programme Molecular Principles of Viruses General Microbiology 2021-08-25T10:15:02Z 12 application/pdf http://hdl.handle.net/10138/333562 eng eng American Society for Microbiology 10.1128/AEM.00929-21 Eronen-Rasimus , E L , Hultman , J , Hai , T , Pessi , I S , Collins , E , Wright , S , Laine , P , Viitamäki , S , Lyra , C , Thomas , D N , Golyshin , P , Luhtanen , A-M , Kuosa , H & Kaartokallio , H 2021 , ' Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature ' , Applied and Environmental Microbiology , vol. 87 , no. 17 , ARTN e00929-21 . https://doi.org/10.1128/AEM.00929-21 ORCID: /0000-0001-8832-5907/work/98975859 ORCID: /0000-0002-2427-6162/work/98976274 ORCID: /0000-0001-5926-7496/work/157598526 http://hdl.handle.net/10138/333562 aec232c9-8028-4432-99b9-171453f24133 000693759300020 cc_by info:eu-repo/semantics/openAccess openAccess 11832 Microbiology and virology Halomonas Paracoccus poly-3-hydroxyalkanoic acid PHA SCL-PHA MCL-PHA copolymer sea-ice bacteria marine bacteria transcriptomics genomics CHAIN-LENGTH CONSTITUENTS POLYHYDROXYALKANOIC ACID 3-HYDROXYBUTYRIC ACID READ ALIGNMENT SYNTHASE BIOSYNTHESIS PATHWAY GENES POLY(3-HYDROXYALKANOATES) POLYHYDROXYBUTYRATE Article publishedVersion 2021 ftunivhelsihelda 2024-04-17T14:29:09Z Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4 degrees C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. Peer reviewed Article in Journal/Newspaper Sea ice Southern Ocean HELDA – University of Helsinki Open Repository Southern Ocean Applied and Environmental Microbiology 87 17 |