Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels
Calcium channels are necessary for cardiac excitation-contraction (E-C) coupling, but Ca2+ channel composition of fish hearts is still largely unknown. To this end, we determined transcript expression of Ca2+ channels in the heart of zebrafish (Danio rerio), a popular model species. Altogether, 18 C...
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/304249 2024-01-07T09:42:30+01:00 Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels Haverinen, Jaakko Hassinen, Minna Dash, Surjya Narayan Vornanen, Matti Medicum Neuroscience Center Department of Anatomy Mitochondrial Morphogenesis 2019-08-02T21:36:51Z 10 application/pdf http://hdl.handle.net/10138/304249 eng eng Company of Biologists Ltd 10.1242/jeb.179226 This work was supported by Jane ja Aatos Erkon Saatio (project no. 64579 to M.V.). Haverinen , J , Hassinen , M , Dash , S N & Vornanen , M 2018 , ' Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels ' , Journal of Experimental Biology , vol. 221 , no. 10 , 179226 . https://doi.org/10.1242/jeb.179226 85047611561 e195b5cf-3651-47ac-a569-65a56d4c79ae http://hdl.handle.net/10138/304249 000438913100018 unspecified openAccess info:eu-repo/semantics/openAccess Danio rerio E-C coupling Cardiac myocytes T-type Ca2+ current Zebrafish heart LEFT-VENTRICULAR MYOCYTES FISH CARDIAC MYOCYTES BURBOT LOTA-LOTA CA2+ CHANNELS SARCOPLASMIC-RETICULUM DRUG DISCOVERY SINOATRIAL NODE ADULT ZEBRAFISH INDUCED RELEASE DANIO-RERIO 3111 Biomedicine Article publishedVersion 2019 ftunivhelsihelda 2023-12-14T00:10:33Z Calcium channels are necessary for cardiac excitation-contraction (E-C) coupling, but Ca2+ channel composition of fish hearts is still largely unknown. To this end, we determined transcript expression of Ca2+ channels in the heart of zebrafish (Danio rerio), a popular model species. Altogether, 18 Ca2+ channel alpha-subunit genes were expressed in both atrium and ventricle. Transcripts for 7 L-type (Ca(v)1.1a, Ca(v)1.1b, Ca(v)1.2, Ca(v)1.3a, Ca(v)1.3b, Ca(v)1.4a, Ca(v)1.4b), 5 T-type (Ca(v)3.1, Ca(v)3.2a, Ca(v)3.2b, Ca(v)3.3a, Ca(v)3.3b) and 6 P/Q-, N - and R-type (Ca(v)2.1a, Ca(v)2.1b, Ca(v)2.2a, Ca(v)2.2b, Ca(v)2.3a, Ca(v)2.3b) Ca2+ channels were expressed. In the ventricle, T-type channels formed 54.9%, L-type channels 41.1% and P/Q-, N- and R-type channels 4.0% of the Ca2+ channel transcripts. In the atrium, the relative expression of T-type and L-type Ca2+ channel transcripts was 64.1% and 33.8%, respectively (others accounted for 2.1%). Thus, at the transcript level, T-type Ca2+ channels are prevalent in zebrafish atrium and ventricle. At the functional level, peak densities of ventricular T-type (I-CAT) and L-type (I-CAL) Ca2+ current were 6.3 +/- 0.8 and 7.7 +/- 0.8 pA pF(-1), respectively. I-CAT mediated a sizeable sarcolemmal Ca2+ influx into ventricular myocytes: the increment in total cellular Ca2+ content via I-CAT was 41.2 +/- 7.3 mu mol l(-1), which was 31.7% of the combined Ca2+ influx (129 mu mol l(-1)) via I(CAT )and I(CAL)( ()88.5 +/- 20.5 mu mol l(-1)). The diversity of expressed Ca2+ channel genes in zebrafish heart is high, but dominated by the members of the T-type subfamily. The large ventricular I(CAT )is likely to play a significant role in E-C coupling. Peer reviewed Article in Journal/Newspaper Burbot Lota lota lota HELDA – University of Helsinki Open Repository Journal of Experimental Biology |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
Danio rerio E-C coupling Cardiac myocytes T-type Ca2+ current Zebrafish heart LEFT-VENTRICULAR MYOCYTES FISH CARDIAC MYOCYTES BURBOT LOTA-LOTA CA2+ CHANNELS SARCOPLASMIC-RETICULUM DRUG DISCOVERY SINOATRIAL NODE ADULT ZEBRAFISH INDUCED RELEASE DANIO-RERIO 3111 Biomedicine |
spellingShingle |
Danio rerio E-C coupling Cardiac myocytes T-type Ca2+ current Zebrafish heart LEFT-VENTRICULAR MYOCYTES FISH CARDIAC MYOCYTES BURBOT LOTA-LOTA CA2+ CHANNELS SARCOPLASMIC-RETICULUM DRUG DISCOVERY SINOATRIAL NODE ADULT ZEBRAFISH INDUCED RELEASE DANIO-RERIO 3111 Biomedicine Haverinen, Jaakko Hassinen, Minna Dash, Surjya Narayan Vornanen, Matti Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
topic_facet |
Danio rerio E-C coupling Cardiac myocytes T-type Ca2+ current Zebrafish heart LEFT-VENTRICULAR MYOCYTES FISH CARDIAC MYOCYTES BURBOT LOTA-LOTA CA2+ CHANNELS SARCOPLASMIC-RETICULUM DRUG DISCOVERY SINOATRIAL NODE ADULT ZEBRAFISH INDUCED RELEASE DANIO-RERIO 3111 Biomedicine |
description |
Calcium channels are necessary for cardiac excitation-contraction (E-C) coupling, but Ca2+ channel composition of fish hearts is still largely unknown. To this end, we determined transcript expression of Ca2+ channels in the heart of zebrafish (Danio rerio), a popular model species. Altogether, 18 Ca2+ channel alpha-subunit genes were expressed in both atrium and ventricle. Transcripts for 7 L-type (Ca(v)1.1a, Ca(v)1.1b, Ca(v)1.2, Ca(v)1.3a, Ca(v)1.3b, Ca(v)1.4a, Ca(v)1.4b), 5 T-type (Ca(v)3.1, Ca(v)3.2a, Ca(v)3.2b, Ca(v)3.3a, Ca(v)3.3b) and 6 P/Q-, N - and R-type (Ca(v)2.1a, Ca(v)2.1b, Ca(v)2.2a, Ca(v)2.2b, Ca(v)2.3a, Ca(v)2.3b) Ca2+ channels were expressed. In the ventricle, T-type channels formed 54.9%, L-type channels 41.1% and P/Q-, N- and R-type channels 4.0% of the Ca2+ channel transcripts. In the atrium, the relative expression of T-type and L-type Ca2+ channel transcripts was 64.1% and 33.8%, respectively (others accounted for 2.1%). Thus, at the transcript level, T-type Ca2+ channels are prevalent in zebrafish atrium and ventricle. At the functional level, peak densities of ventricular T-type (I-CAT) and L-type (I-CAL) Ca2+ current were 6.3 +/- 0.8 and 7.7 +/- 0.8 pA pF(-1), respectively. I-CAT mediated a sizeable sarcolemmal Ca2+ influx into ventricular myocytes: the increment in total cellular Ca2+ content via I-CAT was 41.2 +/- 7.3 mu mol l(-1), which was 31.7% of the combined Ca2+ influx (129 mu mol l(-1)) via I(CAT )and I(CAL)( ()88.5 +/- 20.5 mu mol l(-1)). The diversity of expressed Ca2+ channel genes in zebrafish heart is high, but dominated by the members of the T-type subfamily. The large ventricular I(CAT )is likely to play a significant role in E-C coupling. Peer reviewed |
author2 |
Medicum Neuroscience Center Department of Anatomy Mitochondrial Morphogenesis |
format |
Article in Journal/Newspaper |
author |
Haverinen, Jaakko Hassinen, Minna Dash, Surjya Narayan Vornanen, Matti |
author_facet |
Haverinen, Jaakko Hassinen, Minna Dash, Surjya Narayan Vornanen, Matti |
author_sort |
Haverinen, Jaakko |
title |
Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
title_short |
Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
title_full |
Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
title_fullStr |
Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
title_full_unstemmed |
Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels |
title_sort |
expression of calcium channel transcripts in the zebrafish heart : dominance of t-type channels |
publisher |
Company of Biologists Ltd |
publishDate |
2019 |
url |
http://hdl.handle.net/10138/304249 |
genre |
Burbot Lota lota lota |
genre_facet |
Burbot Lota lota lota |
op_relation |
10.1242/jeb.179226 This work was supported by Jane ja Aatos Erkon Saatio (project no. 64579 to M.V.). Haverinen , J , Hassinen , M , Dash , S N & Vornanen , M 2018 , ' Expression of calcium channel transcripts in the zebrafish heart : dominance of T-type channels ' , Journal of Experimental Biology , vol. 221 , no. 10 , 179226 . https://doi.org/10.1242/jeb.179226 85047611561 e195b5cf-3651-47ac-a569-65a56d4c79ae http://hdl.handle.net/10138/304249 000438913100018 |
op_rights |
unspecified openAccess info:eu-repo/semantics/openAccess |
container_title |
Journal of Experimental Biology |
_version_ |
1787423498689314816 |