The first known virus isolates from Antarctic sea ice have complex infection patterns
Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isola...
Published in: | FEMS Microbiology Ecology |
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Oxford University Press
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Online Access: | http://hdl.handle.net/10138/300595 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/300595 2024-01-07T09:39:38+01:00 The first known virus isolates from Antarctic sea ice have complex infection patterns Luhtanen, Anne-Mari Eronen-Rasimus, Eeva Liisa Oksanen, Hanna Maarit Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard S Rintala, Janne-Markus Bamford, Dennis Henry Biosciences Elina Roine / Principal Investigator Structure of the Viral Universe Molecular and Integrative Biosciences Research Programme Tvärminne Zoological Station Molecular Principles of Viruses Environmental Sciences Aerovirology Research Group 2019-03-31T21:50:14Z 15 application/pdf http://hdl.handle.net/10138/300595 eng eng Oxford University Press 10.1093/femsec/fiy028 Luhtanen , A-M , Eronen-Rasimus , E L , Oksanen , H M , Tison , J-L , Delille , B , Dieckmann , G S , Rintala , J-M & Bamford , D H 2018 , ' The first known virus isolates from Antarctic sea ice have complex infection patterns ' , FEMS Microbiology Ecology , vol. 94 , no. 4 , 028 . https://doi.org/10.1093/femsec/fiy028 ORCID: /0000-0002-6438-8118/work/53824987 ORCID: /0000-0003-3047-8294/work/53828131 85043791422 732e6858-8396-411f-a477-982c3322cbd2 http://hdl.handle.net/10138/300595 000429481200008 openAccess info:eu-repo/semantics/openAccess 1183 Plant biology microbiology virology 1181 Ecology evolutionary biology virus-host interactions virus isolation sea ice microbiology VLP in sea ice BACTERIAL-GROWTH RATE PHAGE-HOST SYSTEMS MARINE-BACTERIA SOUTHERN-OCEAN PACK ICE PSYCHROPHILIC BACTERIA PHYLOGENETIC ANALYSES PROKARYOTIC VIRUSES VIRAL ABUNDANCE WEDDELL SEA Article acceptedVersion 2019 ftunivhelsihelda 2023-12-14T00:06:58Z Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice. Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Antarctica Sea ice Southern Ocean Weddell Sea HELDA – University of Helsinki Open Repository Antarctic Southern Ocean Weddell Sea Weddell FEMS Microbiology Ecology 94 4 |
institution |
Open Polar |
collection |
HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
1183 Plant biology microbiology virology 1181 Ecology evolutionary biology virus-host interactions virus isolation sea ice microbiology VLP in sea ice BACTERIAL-GROWTH RATE PHAGE-HOST SYSTEMS MARINE-BACTERIA SOUTHERN-OCEAN PACK ICE PSYCHROPHILIC BACTERIA PHYLOGENETIC ANALYSES PROKARYOTIC VIRUSES VIRAL ABUNDANCE WEDDELL SEA |
spellingShingle |
1183 Plant biology microbiology virology 1181 Ecology evolutionary biology virus-host interactions virus isolation sea ice microbiology VLP in sea ice BACTERIAL-GROWTH RATE PHAGE-HOST SYSTEMS MARINE-BACTERIA SOUTHERN-OCEAN PACK ICE PSYCHROPHILIC BACTERIA PHYLOGENETIC ANALYSES PROKARYOTIC VIRUSES VIRAL ABUNDANCE WEDDELL SEA Luhtanen, Anne-Mari Eronen-Rasimus, Eeva Liisa Oksanen, Hanna Maarit Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard S Rintala, Janne-Markus Bamford, Dennis Henry The first known virus isolates from Antarctic sea ice have complex infection patterns |
topic_facet |
1183 Plant biology microbiology virology 1181 Ecology evolutionary biology virus-host interactions virus isolation sea ice microbiology VLP in sea ice BACTERIAL-GROWTH RATE PHAGE-HOST SYSTEMS MARINE-BACTERIA SOUTHERN-OCEAN PACK ICE PSYCHROPHILIC BACTERIA PHYLOGENETIC ANALYSES PROKARYOTIC VIRUSES VIRAL ABUNDANCE WEDDELL SEA |
description |
Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice. Peer reviewed |
author2 |
Biosciences Elina Roine / Principal Investigator Structure of the Viral Universe Molecular and Integrative Biosciences Research Programme Tvärminne Zoological Station Molecular Principles of Viruses Environmental Sciences Aerovirology Research Group |
format |
Article in Journal/Newspaper |
author |
Luhtanen, Anne-Mari Eronen-Rasimus, Eeva Liisa Oksanen, Hanna Maarit Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard S Rintala, Janne-Markus Bamford, Dennis Henry |
author_facet |
Luhtanen, Anne-Mari Eronen-Rasimus, Eeva Liisa Oksanen, Hanna Maarit Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard S Rintala, Janne-Markus Bamford, Dennis Henry |
author_sort |
Luhtanen, Anne-Mari |
title |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_short |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_full |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_fullStr |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_full_unstemmed |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_sort |
first known virus isolates from antarctic sea ice have complex infection patterns |
publisher |
Oxford University Press |
publishDate |
2019 |
url |
http://hdl.handle.net/10138/300595 |
geographic |
Antarctic Southern Ocean Weddell Sea Weddell |
geographic_facet |
Antarctic Southern Ocean Weddell Sea Weddell |
genre |
Antarc* Antarctic Antarctica Sea ice Southern Ocean Weddell Sea |
genre_facet |
Antarc* Antarctic Antarctica Sea ice Southern Ocean Weddell Sea |
op_relation |
10.1093/femsec/fiy028 Luhtanen , A-M , Eronen-Rasimus , E L , Oksanen , H M , Tison , J-L , Delille , B , Dieckmann , G S , Rintala , J-M & Bamford , D H 2018 , ' The first known virus isolates from Antarctic sea ice have complex infection patterns ' , FEMS Microbiology Ecology , vol. 94 , no. 4 , 028 . https://doi.org/10.1093/femsec/fiy028 ORCID: /0000-0002-6438-8118/work/53824987 ORCID: /0000-0003-3047-8294/work/53828131 85043791422 732e6858-8396-411f-a477-982c3322cbd2 http://hdl.handle.net/10138/300595 000429481200008 |
op_rights |
openAccess info:eu-repo/semantics/openAccess |
container_title |
FEMS Microbiology Ecology |
container_volume |
94 |
container_issue |
4 |
_version_ |
1787429852951871488 |