Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy
STUDY QUESTION: Does chemotherapy exposure (with or without alkylating agents) or primary diagnosis affect spermatogonial quantity in human prepubertal testicular tissue? SUMMARY ANSWER: Spermatogonial quantity is significantly reduced in testes of prepubertal boys treated with alkylating agent ther...
Published in: | Human Reproduction |
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Main Authors: | , , , , , , , , , , , , , , , , , |
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Language: | English |
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Oxford University Press
2019
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Online Access: | http://hdl.handle.net/10138/288214 |
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fertility preservation childhood cancer sickle cell disease alkylating agents spermatogonial quantity SICKLE-CELL-DISEASE CANCER-TREATMENT WORKING PARTY CHILDHOOD CHILDREN HYDROXYUREA CRYOPRESERVATION TRANSPLANTATION ADOLESCENTS EBMT 3123 Gynaecology and paediatrics |
spellingShingle |
fertility preservation childhood cancer sickle cell disease alkylating agents spermatogonial quantity SICKLE-CELL-DISEASE CANCER-TREATMENT WORKING PARTY CHILDHOOD CHILDREN HYDROXYUREA CRYOPRESERVATION TRANSPLANTATION ADOLESCENTS EBMT 3123 Gynaecology and paediatrics Stukenborg, J. -B. Alves-Lopes, J. P. Kurek, M. Albalushi, H. Reda, A. Keros, V. Töhönen, V. Bjarnason, R. Romerius, P. Sundin, M. Nyström, U. Noren Langenskiöld, C. Vogt, H. Henningsohn, L. Mitchell, R. T. Söder, O. Petersen, C. Jahnukainen, K. Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
topic_facet |
fertility preservation childhood cancer sickle cell disease alkylating agents spermatogonial quantity SICKLE-CELL-DISEASE CANCER-TREATMENT WORKING PARTY CHILDHOOD CHILDREN HYDROXYUREA CRYOPRESERVATION TRANSPLANTATION ADOLESCENTS EBMT 3123 Gynaecology and paediatrics |
description |
STUDY QUESTION: Does chemotherapy exposure (with or without alkylating agents) or primary diagnosis affect spermatogonial quantity in human prepubertal testicular tissue? SUMMARY ANSWER: Spermatogonial quantity is significantly reduced in testes of prepubertal boys treated with alkylating agent therapies or with hydroxyurea for sickle cell disease. WHAT IS KNOWN ALREADY: Cryopreservation of spermatogonial stem cells, followed by transplantation into the testis after treatment, is a proposed clinical option for fertility restoration in children. The key clinical consideration behind this approach is a sufficient quantity of healthy cryopreserved spermatogonia. However, since most boys with malignancies start therapy with agents that are not potentially sterilizing, they will have already received some chemotherapy before testicular tissue cryopreservation is considered. STUDY DESIGN, SIZE, DURATION: We examined histological sections of prepubertal testicular tissue to elucidate whether chemotherapy exposure or primary diagnosis affects spermatogonial quantity. Quantity of spermatogonia per transverse tubular cross-section (S/T) was assessed in relation to treatment characteristics and normative reference values in histological sections of paraffin embedded testicular tissue samples collected from 32 consecutive boy patients (aged 6.3 +/- 3.8 [mean +/- SD] years) between 2014 and 2017, as part of the NORDFERTIL study, and in 14 control samples (from boys aged 5.6 +/- 5.0 [mean +/- SD] years) from an internal biobank. PARTICIPANTS/MATERIALS, SETTING, METHODS: Prepubertal boys in Sweden, Finland and Iceland who were facing treatments associated with a very high risk of infertility, were offered the experimental procedure of testicular cryopreservation. Exclusion criteria were testicular volumes > 10 ml and high bleeding or infection risk. There were 18 patients with a diagnosis of malignancy and 14 patients a nonmalignant diagnosis. While 20 patients had the testicular biopsy performed 1-45 days after ... |
author2 |
Children's Hospital Clinicum University of Helsinki HUS Children and Adolescents |
format |
Article in Journal/Newspaper |
author |
Stukenborg, J. -B. Alves-Lopes, J. P. Kurek, M. Albalushi, H. Reda, A. Keros, V. Töhönen, V. Bjarnason, R. Romerius, P. Sundin, M. Nyström, U. Noren Langenskiöld, C. Vogt, H. Henningsohn, L. Mitchell, R. T. Söder, O. Petersen, C. Jahnukainen, K. |
author_facet |
Stukenborg, J. -B. Alves-Lopes, J. P. Kurek, M. Albalushi, H. Reda, A. Keros, V. Töhönen, V. Bjarnason, R. Romerius, P. Sundin, M. Nyström, U. Noren Langenskiöld, C. Vogt, H. Henningsohn, L. Mitchell, R. T. Söder, O. Petersen, C. Jahnukainen, K. |
author_sort |
Stukenborg, J. -B. |
title |
Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
title_short |
Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
title_full |
Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
title_fullStr |
Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
title_full_unstemmed |
Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
title_sort |
spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy |
publisher |
Oxford University Press |
publishDate |
2019 |
url |
http://hdl.handle.net/10138/288214 |
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ENVELOPE(-66.783,-66.783,-68.867,-68.867) |
geographic |
Sickle |
geographic_facet |
Sickle |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
10.1093/humrep/dey240 This study was supported by grants from The Swedish Childhood Cancer Foundation (PR2016-0124; TJ2016-0093; PR2015-0073, TJ2015-0046) (K.J. and J.-B.S.), the Jane and Dan Olssons Foundation (2016-33) (J.-B.S.), the Finnish Cancer Society (K.J.), the Foundation for Paediatric Research (J.-B.S), Kronprinsessan Lovisas Forening For Barnasjukvard/Stiftelsen Axel Tielmans Minnesfond, Samariten Foundation (J.-B.S.), the Vare Foundation for Paediatric Cancer Research (K.J.), and Swedish Research Council (2012-6352) (O.S.). R.T.M. was supported by a Wellcome Trust Fellowship (09822). J.P. A.-L. and M.K. were supported by the ITN Marie Curie program 'Growsperm' (EU-FP7-PEOPLE-2013-ITN 603568). Stukenborg , J -B , Alves-Lopes , J P , Kurek , M , Albalushi , H , Reda , A , Keros , V , Töhönen , V , Bjarnason , R , Romerius , P , Sundin , M , Nyström , U N , Langenskiöld , C , Vogt , H , Henningsohn , L , Mitchell , R T , Söder , O , Petersen , C & Jahnukainen , K 2018 , ' Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy ' , Human Reproduction , vol. 33 , no. 9 , pp. 1677-1683 . https://doi.org/10.1093/humrep/dey240 85054967057 3bcf0899-028a-4380-b7ca-9f1c73ffb634 http://hdl.handle.net/10138/288214 000452615200011 |
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cc_by openAccess info:eu-repo/semantics/openAccess |
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Human Reproduction |
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33 |
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1677 |
op_container_end_page |
1683 |
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1787425654784917504 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/288214 2024-01-07T09:44:17+01:00 Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy Stukenborg, J. -B. Alves-Lopes, J. P. Kurek, M. Albalushi, H. Reda, A. Keros, V. Töhönen, V. Bjarnason, R. Romerius, P. Sundin, M. Nyström, U. Noren Langenskiöld, C. Vogt, H. Henningsohn, L. Mitchell, R. T. Söder, O. Petersen, C. Jahnukainen, K. Children's Hospital Clinicum University of Helsinki HUS Children and Adolescents 2019-01-08T12:40:01Z 7 application/pdf http://hdl.handle.net/10138/288214 eng eng Oxford University Press 10.1093/humrep/dey240 This study was supported by grants from The Swedish Childhood Cancer Foundation (PR2016-0124; TJ2016-0093; PR2015-0073, TJ2015-0046) (K.J. and J.-B.S.), the Jane and Dan Olssons Foundation (2016-33) (J.-B.S.), the Finnish Cancer Society (K.J.), the Foundation for Paediatric Research (J.-B.S), Kronprinsessan Lovisas Forening For Barnasjukvard/Stiftelsen Axel Tielmans Minnesfond, Samariten Foundation (J.-B.S.), the Vare Foundation for Paediatric Cancer Research (K.J.), and Swedish Research Council (2012-6352) (O.S.). R.T.M. was supported by a Wellcome Trust Fellowship (09822). J.P. A.-L. and M.K. were supported by the ITN Marie Curie program 'Growsperm' (EU-FP7-PEOPLE-2013-ITN 603568). Stukenborg , J -B , Alves-Lopes , J P , Kurek , M , Albalushi , H , Reda , A , Keros , V , Töhönen , V , Bjarnason , R , Romerius , P , Sundin , M , Nyström , U N , Langenskiöld , C , Vogt , H , Henningsohn , L , Mitchell , R T , Söder , O , Petersen , C & Jahnukainen , K 2018 , ' Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy ' , Human Reproduction , vol. 33 , no. 9 , pp. 1677-1683 . https://doi.org/10.1093/humrep/dey240 85054967057 3bcf0899-028a-4380-b7ca-9f1c73ffb634 http://hdl.handle.net/10138/288214 000452615200011 cc_by openAccess info:eu-repo/semantics/openAccess fertility preservation childhood cancer sickle cell disease alkylating agents spermatogonial quantity SICKLE-CELL-DISEASE CANCER-TREATMENT WORKING PARTY CHILDHOOD CHILDREN HYDROXYUREA CRYOPRESERVATION TRANSPLANTATION ADOLESCENTS EBMT 3123 Gynaecology and paediatrics Article publishedVersion 2019 ftunivhelsihelda 2023-12-14T00:02:52Z STUDY QUESTION: Does chemotherapy exposure (with or without alkylating agents) or primary diagnosis affect spermatogonial quantity in human prepubertal testicular tissue? SUMMARY ANSWER: Spermatogonial quantity is significantly reduced in testes of prepubertal boys treated with alkylating agent therapies or with hydroxyurea for sickle cell disease. WHAT IS KNOWN ALREADY: Cryopreservation of spermatogonial stem cells, followed by transplantation into the testis after treatment, is a proposed clinical option for fertility restoration in children. The key clinical consideration behind this approach is a sufficient quantity of healthy cryopreserved spermatogonia. However, since most boys with malignancies start therapy with agents that are not potentially sterilizing, they will have already received some chemotherapy before testicular tissue cryopreservation is considered. STUDY DESIGN, SIZE, DURATION: We examined histological sections of prepubertal testicular tissue to elucidate whether chemotherapy exposure or primary diagnosis affects spermatogonial quantity. Quantity of spermatogonia per transverse tubular cross-section (S/T) was assessed in relation to treatment characteristics and normative reference values in histological sections of paraffin embedded testicular tissue samples collected from 32 consecutive boy patients (aged 6.3 +/- 3.8 [mean +/- SD] years) between 2014 and 2017, as part of the NORDFERTIL study, and in 14 control samples (from boys aged 5.6 +/- 5.0 [mean +/- SD] years) from an internal biobank. PARTICIPANTS/MATERIALS, SETTING, METHODS: Prepubertal boys in Sweden, Finland and Iceland who were facing treatments associated with a very high risk of infertility, were offered the experimental procedure of testicular cryopreservation. Exclusion criteria were testicular volumes > 10 ml and high bleeding or infection risk. There were 18 patients with a diagnosis of malignancy and 14 patients a nonmalignant diagnosis. While 20 patients had the testicular biopsy performed 1-45 days after ... Article in Journal/Newspaper Iceland HELDA – University of Helsinki Open Repository Sickle ENVELOPE(-66.783,-66.783,-68.867,-68.867) Human Reproduction 33 9 1677 1683 |