Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains
"In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September...
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American Society for Microbiology
2011
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Online Access: | http://hdl.handle.net/10138/24590 |
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/24590 2024-01-07T09:45:27+01:00 Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains Razzauti Sanfeliu, Maria Plyusnina, Angelina Sironen, Tarja Henttonen, Heikki Pljusnin, Aleksandr Department of Virology Viral Zoonosis Research Unit Emerging Infections Research Group 2011-01-19T12:02:16Z 9 application/pdf http://hdl.handle.net/10138/24590 eng eng American Society for Microbiology 10.1099/vir.0.011304-0 Razzauti Sanfeliu , M , Plyusnina , A , Sironen , T , Henttonen , H & Pljusnin , A 2009 , ' Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains ' , Journal of General Virology , vol. 90 , no. 8 , pp. 1923-1931 . https://doi.org/10.1099/vir.0.011304-0 dawa_publication: 198171 ORCID: /0000-0002-2344-2755/work/43226618 69449095191 d3e0929a-375d-41e7-b629-198caf2877a4 http://hdl.handle.net/10138/24590 000268699400015 openAccess info:eu-repo/semantics/openAccess Article acceptedVersion 2011 ftunivhelsihelda 2023-12-14T00:13:31Z "In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September 1998 were screened for the presence of PUUV nucleocapsid antigen and 25 were found to be positive. Partial sequences of the PUUV small (S), medium (M) and large (L) genome segments were recovered from these samples using RT-PCR. Phylogenetic analysis revealed two genetic groups of PUUV sequences that belonged to the Finnish and north Scandinavian lineages. This presented a unique opportunity to study inter-lineage reassortment in PUUV; indeed, 32% of the studied bank voles appeared to carry reassortant virus genomes. Thus, the frequency of inter-lineage reassortment in PUUV was comparable to that of intra-lineage reassortment observed previously (Razzauti, M., Plyusnina, A., Henttonen, H. & Plyusnin, A. (2008). J Gen Virol 89, 1649-1660). Of six possible reassortant S/M/L combinations, only two were found at Pallasjarvi and, notably, in all reassortants, both S and L segments originated from the same genetic lineage, suggesting a non-random pattern for the reassortment. These findings are discussed in connection to PUUV evolution in Fermoscandia." "In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September 1998 were screened for the presence of PUUV nucleocapsid antigen and 25 were found to be positive. Partial sequences of the PUUV small (S), medium (M) and large (L) genome segments were recovered from these samples using RT-PCR. Phylogenetic analysis revealed two genetic groups of PUUV sequences that belonged to the Finnish and north Scandinavian lineages. This presented a unique ... Article in Journal/Newspaper Northern Finland HELDA – University of Helsinki Open Repository Journal of General Virology 90 8 1923 1931 |
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Open Polar |
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HELDA – University of Helsinki Open Repository |
op_collection_id |
ftunivhelsihelda |
language |
English |
description |
"In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September 1998 were screened for the presence of PUUV nucleocapsid antigen and 25 were found to be positive. Partial sequences of the PUUV small (S), medium (M) and large (L) genome segments were recovered from these samples using RT-PCR. Phylogenetic analysis revealed two genetic groups of PUUV sequences that belonged to the Finnish and north Scandinavian lineages. This presented a unique opportunity to study inter-lineage reassortment in PUUV; indeed, 32% of the studied bank voles appeared to carry reassortant virus genomes. Thus, the frequency of inter-lineage reassortment in PUUV was comparable to that of intra-lineage reassortment observed previously (Razzauti, M., Plyusnina, A., Henttonen, H. & Plyusnin, A. (2008). J Gen Virol 89, 1649-1660). Of six possible reassortant S/M/L combinations, only two were found at Pallasjarvi and, notably, in all reassortants, both S and L segments originated from the same genetic lineage, suggesting a non-random pattern for the reassortment. These findings are discussed in connection to PUUV evolution in Fermoscandia." "In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September 1998 were screened for the presence of PUUV nucleocapsid antigen and 25 were found to be positive. Partial sequences of the PUUV small (S), medium (M) and large (L) genome segments were recovered from these samples using RT-PCR. Phylogenetic analysis revealed two genetic groups of PUUV sequences that belonged to the Finnish and north Scandinavian lineages. This presented a unique ... |
author2 |
Department of Virology Viral Zoonosis Research Unit Emerging Infections Research Group |
format |
Article in Journal/Newspaper |
author |
Razzauti Sanfeliu, Maria Plyusnina, Angelina Sironen, Tarja Henttonen, Heikki Pljusnin, Aleksandr |
spellingShingle |
Razzauti Sanfeliu, Maria Plyusnina, Angelina Sironen, Tarja Henttonen, Heikki Pljusnin, Aleksandr Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
author_facet |
Razzauti Sanfeliu, Maria Plyusnina, Angelina Sironen, Tarja Henttonen, Heikki Pljusnin, Aleksandr |
author_sort |
Razzauti Sanfeliu, Maria |
title |
Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
title_short |
Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
title_full |
Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
title_fullStr |
Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
title_full_unstemmed |
Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
title_sort |
analysis of puumala hantavirus in a bank vole population in northern finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://hdl.handle.net/10138/24590 |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_relation |
10.1099/vir.0.011304-0 Razzauti Sanfeliu , M , Plyusnina , A , Sironen , T , Henttonen , H & Pljusnin , A 2009 , ' Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains ' , Journal of General Virology , vol. 90 , no. 8 , pp. 1923-1931 . https://doi.org/10.1099/vir.0.011304-0 dawa_publication: 198171 ORCID: /0000-0002-2344-2755/work/43226618 69449095191 d3e0929a-375d-41e7-b629-198caf2877a4 http://hdl.handle.net/10138/24590 000268699400015 |
op_rights |
openAccess info:eu-repo/semantics/openAccess |
container_title |
Journal of General Virology |
container_volume |
90 |
container_issue |
8 |
container_start_page |
1923 |
op_container_end_page |
1931 |
_version_ |
1787426979733045248 |