Chemical Profiling and Bioactivity of Body Wall Lipids from Strongylocentrotus droebachiensis

• Published in: Marine Drugs
• Main Authors: Shikov, Alexander N., Laakso, Into, Pozharitskaya, Olga N., Seppänen-Laakso, Tuulikki, Krishtopina, Anna S., Makarova, Marina N., Vuorela, Heikki, Makarov, Valery
• Other Authors: Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Pharmaceutical Design and Discovery group, Drug Research Program
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI 2018
• Subjects: sea urchin; body wall lipids; inhibition of p38 MAPK; COX; GC-MS; UPLC-ELSD; POLYUNSATURATED FATTY-ACIDS; SEA-URCHIN; EICOSAPENTAENOIC ACID; GAS-CHROMATOGRAPHY; ENDOTHELIAL-CELLS; COX-2 EXPRESSION; DIETARY LIPIDS; BARENTS SEA; LIVER OIL; SHELLS; 317 Pharmacy; 116 Chemical sciences; Barents Sea
• Online Access: http://hdl.handle.net/10138/231264

The lipids from gonads and polyhydroxynaphthoquinone pigments from body walls of sea urchins are intensively studied. However, little is known about the body wall (BW) lipids. Ethanol extract (55 degrees C) contained about equal amounts of saturated (SaFA) and monounsaturated fatty acids (MUFA) representing 60% of total fatty acids, with myristic, palmitic and eicosenoic acids as major SaFAs and MUFAs, respectively. Non-methylene-interrupted dienes (13%) were composed of eicosadienoic and docosadienoic acids. Long-chain polyunsaturated fatty acids (LC-PUFA) included two main components, n6 arachidonic and n3 eicosapentaenoic acids, even with equal concentrations (15 mu g/mg) and a balanced n6/n3 PUFA ratio (0.86). The UPLC-ELSD analysis showed that a great majority of the lipids (80%) in the ethanolic extract were phosphatidylcholine (60 mu g/mg) and phosphatidylethanolamine (40 mu g/mg), while the proportion of neutral lipids remained lower than 20%. In addition, alkoxyglycerol derivativeschimyl, selachyl, and batyl alcoholswere quantified. We have assumed that the mechanism of action of body wall lipids in the present study is via the inhibition of MAPK p38, COX-1, and COX-2. Our findings open the prospective to utilize this lipid fraction as a source for the development of drugs with anti-inflammatory activity. Peer reviewed