Dietary modulation of β-catenin signalling in an experimental model of colon cancer
Colorectal cancer is among the major cancers and one of the leading causes of cancer-related deaths in Western societies. Its occurrence is strongly affected by environmental factors such as diet. Thus, for preventative strategies it is vitally important to understand the mechanisms that stimulate a...
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/20788 2023-08-20T04:09:28+02:00 Dietary modulation of β-catenin signalling in an experimental model of colon cancer Ravinnon vaikutus β-kateniini -signalointiin kokeellisessa paksusuolisyöpämallissa Misikangas, Marjo Mykkänen, Hannu University of Helsinki, Faculty of Agriculture and Forestry, Department of Applied Chemistry and Microbiology, Nutrition Helsingin yliopisto, maatalous-metsätieteellinen tiedekunta, soveltavan kemian ja mikrobiologian laitos Helsingfors universitet, agrikultur-forstvetenskapliga fakulteten, institutionen för tillämpad kemi och mikrobiologi Mutanen, Marja 2010-11-25T11:44:30Z application/pdf http://hdl.handle.net/10138/20788 eng eng Helsingin yliopisto Helsingfors universitet University of Helsinki URN:ISBN:978-952-92-3103-4 Helsinki: Marjo Misikangas, 2007 http://hdl.handle.net/10138/20788 URN:ISBN:978-952-10-4431-1 Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. ravitsemustiede Text Doctoral dissertation (article-based) Artikkeliväitöskirja Artikelavhandling doctoralThesis 2010 ftunivhelsihelda 2023-07-28T06:18:10Z Colorectal cancer is among the major cancers and one of the leading causes of cancer-related deaths in Western societies. Its occurrence is strongly affected by environmental factors such as diet. Thus, for preventative strategies it is vitally important to understand the mechanisms that stimulate adenoma growth and development towards accelerated malignancy or, in contrast, attenuate them to remain in quiescence for periods as long as decades. The main objective of this study was to investigate whether diet is able to modulate β-catenin signalling related to the promotion or prevention of intestinal tumourigenesis in an animal model of colon cancer, the Min/+ mouse. A series of dietary experiments with Min/+ mice were performed where fructo-oligosaccharide inulin was used for tumour promotion and four berries, bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cloudberry (Rubus chamaemorus) and white currant (Ribes x pallidum), were used for tumour prevention. The adenomas (Apc-/-) and surrounding normal-appearing mucosa (Apc+/-) were investigated separately due to their mutational and functional differences. Tumour promotive and preventive diets had opposite effects on β-catenin signalling in the adenomas that was related to the different adenoma growth effects of dietary inulin and berries. The levels of nuclear β-catenin and cyclin D1 combined with size of the adenomas in the treatment groups suggests that diets induced differences in the cancerous process. Adenomas progressing to malignant carcinomas are most likely found in the sub-groups having the highest levels of β-catenin. On the other hand, adenomas staying quiescent for a long period of time are most probably found in the cloudberry or white currant diet groups. The levels of membranous E-cadherin and β-catenin increased as the adenomas in the inulin diet group grew, which could be a result of the overall increase in the protein levels of the cell. Therefore, the increasing levels of membranous β-catenin in Min/+ mice adenomas ... Doctoral or Postdoctoral Thesis Rubus chamaemorus Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto Ribes ENVELOPE(-60.633,-60.633,-62.650,-62.650) |
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Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
ravitsemustiede |
spellingShingle |
ravitsemustiede Misikangas, Marjo Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
topic_facet |
ravitsemustiede |
description |
Colorectal cancer is among the major cancers and one of the leading causes of cancer-related deaths in Western societies. Its occurrence is strongly affected by environmental factors such as diet. Thus, for preventative strategies it is vitally important to understand the mechanisms that stimulate adenoma growth and development towards accelerated malignancy or, in contrast, attenuate them to remain in quiescence for periods as long as decades. The main objective of this study was to investigate whether diet is able to modulate β-catenin signalling related to the promotion or prevention of intestinal tumourigenesis in an animal model of colon cancer, the Min/+ mouse. A series of dietary experiments with Min/+ mice were performed where fructo-oligosaccharide inulin was used for tumour promotion and four berries, bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cloudberry (Rubus chamaemorus) and white currant (Ribes x pallidum), were used for tumour prevention. The adenomas (Apc-/-) and surrounding normal-appearing mucosa (Apc+/-) were investigated separately due to their mutational and functional differences. Tumour promotive and preventive diets had opposite effects on β-catenin signalling in the adenomas that was related to the different adenoma growth effects of dietary inulin and berries. The levels of nuclear β-catenin and cyclin D1 combined with size of the adenomas in the treatment groups suggests that diets induced differences in the cancerous process. Adenomas progressing to malignant carcinomas are most likely found in the sub-groups having the highest levels of β-catenin. On the other hand, adenomas staying quiescent for a long period of time are most probably found in the cloudberry or white currant diet groups. The levels of membranous E-cadherin and β-catenin increased as the adenomas in the inulin diet group grew, which could be a result of the overall increase in the protein levels of the cell. Therefore, the increasing levels of membranous β-catenin in Min/+ mice adenomas ... |
author2 |
Mykkänen, Hannu University of Helsinki, Faculty of Agriculture and Forestry, Department of Applied Chemistry and Microbiology, Nutrition Helsingin yliopisto, maatalous-metsätieteellinen tiedekunta, soveltavan kemian ja mikrobiologian laitos Helsingfors universitet, agrikultur-forstvetenskapliga fakulteten, institutionen för tillämpad kemi och mikrobiologi Mutanen, Marja |
format |
Doctoral or Postdoctoral Thesis |
author |
Misikangas, Marjo |
author_facet |
Misikangas, Marjo |
author_sort |
Misikangas, Marjo |
title |
Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
title_short |
Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
title_full |
Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
title_fullStr |
Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
title_full_unstemmed |
Dietary modulation of β-catenin signalling in an experimental model of colon cancer |
title_sort |
dietary modulation of β-catenin signalling in an experimental model of colon cancer |
publisher |
Helsingin yliopisto |
publishDate |
2010 |
url |
http://hdl.handle.net/10138/20788 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.650,-62.650) |
geographic |
Ribes |
geographic_facet |
Ribes |
genre |
Rubus chamaemorus |
genre_facet |
Rubus chamaemorus |
op_relation |
URN:ISBN:978-952-92-3103-4 Helsinki: Marjo Misikangas, 2007 http://hdl.handle.net/10138/20788 URN:ISBN:978-952-10-4431-1 |
op_rights |
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. |
_version_ |
1774722451852230656 |