Sindbis virus and Pogosta disease in Finland
Sindbis virus (SINV) (genus Alphavirus, family Togaviridae) is an enveloped virus with a genome of single-stranded, positive-polarity RNA of 11.7 kilobases. SINV is widespread in Eurasia, Africa, and Australia, but clinical infection only occurs in a few geographically restricted areas, mainly in No...
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ftunivhelsihelda:oai:helda.helsinki.fi:10138/20554 2023-08-20T04:07:41+02:00 Sindbis virus and Pogosta disease in Finland Sindbis-virus ja pogostantauti Suomessa Kurkela, Satu Gessain, Antoine University of Helsinki, Faculty of Medicine, Haartman Institute, Department of Virology Helsingin yliopisto, lääketieteellinen tiedekunta, kliinisteoreettinen laitos Helsingfors universitet, medicinska fakulteten, Haartman institutet Vapalahti, Olli Vaheri, Antti 2010-11-25T11:28:23Z application/pdf http://hdl.handle.net/10138/20554 eng eng Helsingin yliopisto Helsingfors universitet University of Helsinki URN:ISBN:978-952-92-2880-5 Helsinki: Satu Kurkela, 2007 http://hdl.handle.net/10138/20554 URN:ISBN:978-952-10-4262-1 Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. lääketiede (kliininen virologia) Text Doctoral dissertation (article-based) Artikkeliväitöskirja Artikelavhandling doctoralThesis 2010 ftunivhelsihelda 2023-07-28T06:13:32Z Sindbis virus (SINV) (genus Alphavirus, family Togaviridae) is an enveloped virus with a genome of single-stranded, positive-polarity RNA of 11.7 kilobases. SINV is widespread in Eurasia, Africa, and Australia, but clinical infection only occurs in a few geographically restricted areas, mainly in Northern Europe. In Europe, antibodies to SINV were detected from patients with fever, rash, and arthritis for the first time in the early 1980s in Finland. It became evident that the causative agent of this syndrome, named Pogosta disease, was closely related to SINV. The disease is also found in Sweden (Ockelbo disease) and in Russia (Karelian fever). Since 1974, for unknown reason, the disease has occurred as large outbreaks every seven years in Finland. This study is to a large degree based on the material collected during the 2002 Pogosta disease outbreak in Finland. We first developed SINV IgM and IgG enzyme immunoassays (EIA), based on highly purified SINV, to be used in serodiagnostics. The EIAs correlated well with the hemagglutination inhibition (HI) test, and all individuals showed neutralizing antibodies. The sensitivities of the IgM and IgG EIAs were 97.6% and 100%, and specificities 95.2% and 97.6%, respectively. E1 and E2 envelope glycoproteins of SINV were shown to be recognized by IgM and IgG in the immunoblot early in infection. We isolated SINV from five patients with acute Pogosta disease; one virus strain was recovered from whole blood, and four other strains from skin lesions. The etiology of Pogosta disease was confirmed by these first Finnish SINV strains, also representing the first human SINV isolates from Europe. Phylogenetic analysis indicated that the Finnish SINV strains clustered with the strains previously isolated from mosquitoes in Sweden and Russia, and seemed to have a common ancestor with South-African strains. Northern European SINV strains could be maintained locally in disease-endemic regions, but the phylogenetic analysis also suggests that redistribution of SINV tends to occur ... Doctoral or Postdoctoral Thesis karelia* karelia* karelian Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto Eia ENVELOPE(7.755,7.755,63.024,63.024) |
institution |
Open Polar |
collection |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
English |
topic |
lääketiede (kliininen virologia) |
spellingShingle |
lääketiede (kliininen virologia) Kurkela, Satu Sindbis virus and Pogosta disease in Finland |
topic_facet |
lääketiede (kliininen virologia) |
description |
Sindbis virus (SINV) (genus Alphavirus, family Togaviridae) is an enveloped virus with a genome of single-stranded, positive-polarity RNA of 11.7 kilobases. SINV is widespread in Eurasia, Africa, and Australia, but clinical infection only occurs in a few geographically restricted areas, mainly in Northern Europe. In Europe, antibodies to SINV were detected from patients with fever, rash, and arthritis for the first time in the early 1980s in Finland. It became evident that the causative agent of this syndrome, named Pogosta disease, was closely related to SINV. The disease is also found in Sweden (Ockelbo disease) and in Russia (Karelian fever). Since 1974, for unknown reason, the disease has occurred as large outbreaks every seven years in Finland. This study is to a large degree based on the material collected during the 2002 Pogosta disease outbreak in Finland. We first developed SINV IgM and IgG enzyme immunoassays (EIA), based on highly purified SINV, to be used in serodiagnostics. The EIAs correlated well with the hemagglutination inhibition (HI) test, and all individuals showed neutralizing antibodies. The sensitivities of the IgM and IgG EIAs were 97.6% and 100%, and specificities 95.2% and 97.6%, respectively. E1 and E2 envelope glycoproteins of SINV were shown to be recognized by IgM and IgG in the immunoblot early in infection. We isolated SINV from five patients with acute Pogosta disease; one virus strain was recovered from whole blood, and four other strains from skin lesions. The etiology of Pogosta disease was confirmed by these first Finnish SINV strains, also representing the first human SINV isolates from Europe. Phylogenetic analysis indicated that the Finnish SINV strains clustered with the strains previously isolated from mosquitoes in Sweden and Russia, and seemed to have a common ancestor with South-African strains. Northern European SINV strains could be maintained locally in disease-endemic regions, but the phylogenetic analysis also suggests that redistribution of SINV tends to occur ... |
author2 |
Gessain, Antoine University of Helsinki, Faculty of Medicine, Haartman Institute, Department of Virology Helsingin yliopisto, lääketieteellinen tiedekunta, kliinisteoreettinen laitos Helsingfors universitet, medicinska fakulteten, Haartman institutet Vapalahti, Olli Vaheri, Antti |
format |
Doctoral or Postdoctoral Thesis |
author |
Kurkela, Satu |
author_facet |
Kurkela, Satu |
author_sort |
Kurkela, Satu |
title |
Sindbis virus and Pogosta disease in Finland |
title_short |
Sindbis virus and Pogosta disease in Finland |
title_full |
Sindbis virus and Pogosta disease in Finland |
title_fullStr |
Sindbis virus and Pogosta disease in Finland |
title_full_unstemmed |
Sindbis virus and Pogosta disease in Finland |
title_sort |
sindbis virus and pogosta disease in finland |
publisher |
Helsingin yliopisto |
publishDate |
2010 |
url |
http://hdl.handle.net/10138/20554 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
karelia* karelia* karelian |
genre_facet |
karelia* karelia* karelian |
op_relation |
URN:ISBN:978-952-92-2880-5 Helsinki: Satu Kurkela, 2007 http://hdl.handle.net/10138/20554 URN:ISBN:978-952-10-4262-1 |
op_rights |
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. |
_version_ |
1774719504169828352 |