Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses
Two iridovirus isolates recovered from cod (Gadus morhua) and turbot (Psetta maxima) in Denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (FV3), the reference strain for the genus Ranavirus. The isolates were assessed according to their reactivity in immunofl...
Published in: | Archives of Virology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | Finnish |
Published: |
Springer
2010
|
Subjects: | |
Online Access: | http://hdl.handle.net/10138/17447 |
id |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/17447 |
---|---|
record_format |
openpolar |
spelling |
ftunivhelsihelda:oai:helda.helsinki.fi:10138/17447 2023-08-20T04:06:37+02:00 Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses Ariel, Ellen Holopainen, Riikka Olesen, Niels Jörgen Tapiovaara, Hannele 2010-08-17T08:44:19Z application/pdf http://hdl.handle.net/10138/17447 fin fin Springer https://doi.org/10.1007/s00705-010-0715-z http://hdl.handle.net/10138/17447 Artikkeli 2010 ftunivhelsihelda https://doi.org/10.1007/s00705-010-0715-z 2023-07-28T06:21:03Z Two iridovirus isolates recovered from cod (Gadus morhua) and turbot (Psetta maxima) in Denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (FV3), the reference strain for the genus Ranavirus. The isolates were assessed according to their reactivity in immunofluoresent antibody tests (IFAT) using both homologous and heterologous antisera and their amplification in PCR using primers targeting five genomic regions. The corresponding PCR fragments were sequenced, and the sequences obtained were used in phylogenetic analysis. In addition, the pathogenicity to rainbow trout under experimental challenge conditions was investigated. The viruses were serologically and genetically closely related to highly pathogenic ranaviruses such as European catfish iridovirus (ECV), European sheatfish iridovirus (ESV) and epizootic haematopoietic necrosis virus (EHNV). The challenge trials indicate that rainbow trout fry cultured at 15 C are not target species for the virus isolates in the present panel. We suggest that the two isolates belong in the genus Ranavirus and propose the name Ranavirus maxima (Rmax) for the turbot isolate. Article in Journal/Newspaper Gadus morhua Turbot Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto Archives of Virology 155 8 1261 1271 |
institution |
Open Polar |
collection |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
op_collection_id |
ftunivhelsihelda |
language |
Finnish |
description |
Two iridovirus isolates recovered from cod (Gadus morhua) and turbot (Psetta maxima) in Denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (FV3), the reference strain for the genus Ranavirus. The isolates were assessed according to their reactivity in immunofluoresent antibody tests (IFAT) using both homologous and heterologous antisera and their amplification in PCR using primers targeting five genomic regions. The corresponding PCR fragments were sequenced, and the sequences obtained were used in phylogenetic analysis. In addition, the pathogenicity to rainbow trout under experimental challenge conditions was investigated. The viruses were serologically and genetically closely related to highly pathogenic ranaviruses such as European catfish iridovirus (ECV), European sheatfish iridovirus (ESV) and epizootic haematopoietic necrosis virus (EHNV). The challenge trials indicate that rainbow trout fry cultured at 15 C are not target species for the virus isolates in the present panel. We suggest that the two isolates belong in the genus Ranavirus and propose the name Ranavirus maxima (Rmax) for the turbot isolate. |
format |
Article in Journal/Newspaper |
author |
Ariel, Ellen Holopainen, Riikka Olesen, Niels Jörgen Tapiovaara, Hannele |
spellingShingle |
Ariel, Ellen Holopainen, Riikka Olesen, Niels Jörgen Tapiovaara, Hannele Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
author_facet |
Ariel, Ellen Holopainen, Riikka Olesen, Niels Jörgen Tapiovaara, Hannele |
author_sort |
Ariel, Ellen |
title |
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
title_short |
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
title_full |
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
title_fullStr |
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
title_full_unstemmed |
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses |
title_sort |
comparative study of ranavirus isolates from cod (gadus morhua) and turbot (psetta maxima) with reference to other ranaviruses |
publisher |
Springer |
publishDate |
2010 |
url |
http://hdl.handle.net/10138/17447 |
genre |
Gadus morhua Turbot |
genre_facet |
Gadus morhua Turbot |
op_relation |
https://doi.org/10.1007/s00705-010-0715-z http://hdl.handle.net/10138/17447 |
op_doi |
https://doi.org/10.1007/s00705-010-0715-z |
container_title |
Archives of Virology |
container_volume |
155 |
container_issue |
8 |
container_start_page |
1261 |
op_container_end_page |
1271 |
_version_ |
1774717837052477440 |