Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults...
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ftunivhasselt:oai:documentserver.uhasselt.be:1942/24462 2023-05-15T15:52:53+02:00 Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen 2017 application/pdf http://hdl.handle.net/1942/24462 https://doi.org/10.1186/s12940-017-0292-7 en eng BIOMED CENTRAL LTD ENVIRONMENTAL HEALTH, 16, p. 1-15 (Art N° 87) 1476-069X http://hdl.handle.net/1942/24462 15 1 16 doi:10.1186/s12940-017-0292-7 000408044200001 © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. info:eu-repo/semantics/openAccess CC0 PDM CC-BY Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria info:eu-repo/semantics/article 2017 ftunivhasselt https://doi.org/10.1186/s12940-017-0292-7 2022-11-30T23:21:04Z Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. The project was funded by the Environment, Nature and Energy Department of the Flemish government (LNE/OL201100023/13034/M&G), Steunpunt Milieuen Gezondheid and European Research Council (ERC-2012-StG 310,898). Ellen Winckelmans has a PhD. fellowship of ... Article in Journal/Newspaper Carbonic acid Document Server@UHasselt (Hasselt University) Environmental Health 16 1 |
institution |
Open Polar |
collection |
Document Server@UHasselt (Hasselt University) |
op_collection_id |
ftunivhasselt |
language |
English |
topic |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria |
spellingShingle |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
topic_facet |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria |
description |
Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. The project was funded by the Environment, Nature and Energy Department of the Flemish government (LNE/OL201100023/13034/M&G), Steunpunt Milieuen Gezondheid and European Research Council (ERC-2012-StG 310,898). Ellen Winckelmans has a PhD. fellowship of ... |
author2 |
WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen |
format |
Article in Journal/Newspaper |
author |
WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen |
author_facet |
WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen |
author_sort |
WINCKELMANS, Ellen |
title |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_short |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_fullStr |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full_unstemmed |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_sort |
transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
publisher |
BIOMED CENTRAL LTD |
publishDate |
2017 |
url |
http://hdl.handle.net/1942/24462 https://doi.org/10.1186/s12940-017-0292-7 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
ENVIRONMENTAL HEALTH, 16, p. 1-15 (Art N° 87) 1476-069X http://hdl.handle.net/1942/24462 15 1 16 doi:10.1186/s12940-017-0292-7 000408044200001 |
op_rights |
© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12940-017-0292-7 |
container_title |
Environmental Health |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766387985493786624 |