Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction
In order to stimulate selection for plant-associated bacteria with the potential to improve Cd phytoextraction, yellow lupine plants were grown on a metal-contaminated field soil. It was hypothesised that growing these plants on this contaminated soil, which is a source of bacteria possessing differ...
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2014
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Online Access: | http://hdl.handle.net/1942/17764 https://doi.org/10.1111/plb.12141 |
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ftunivhasselt:oai:documentserver.uhasselt.be:1942/17764 2023-05-15T16:30:25+02:00 Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco 2014 application/pdf http://hdl.handle.net/1942/17764 https://doi.org/10.1111/plb.12141 en eng WILEY-BLACKWELL PLANT BIOLOGY, 16 (5), p. 988-996 1435-8603 http://hdl.handle.net/1942/17764 996 5 988 16 doi:10.1111/plb.12141 000340683100016 © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands. info:eu-repo/semantics/restrictedAccess Cadmium natural selection phytoremediation plant-associated bacteria plant-bacteria interactions plant–bacteria interactions info:eu-repo/semantics/article 2014 ftunivhasselt https://doi.org/10.1111/plb.12141 2023-02-15T23:21:30Z In order to stimulate selection for plant-associated bacteria with the potential to improve Cd phytoextraction, yellow lupine plants were grown on a metal-contaminated field soil. It was hypothesised that growing these plants on this contaminated soil, which is a source of bacteria possessing different traits to cope with Cd, could enhance colonisation of lupine with potential plant-associated bacteria that could then be inoculated in Cd-exposed plants to reduce Cd phytotoxicity and enhance Cd uptake. All cultivable bacteria from rhizosphere, root and stem were isolated and genotypically and phenotypically characterised. Many of the rhizobacteria and root endophytes produce siderophores, organic acids, indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylate (ACC) deaminase, as well as being resistant to Cd and Zn. Most of the stem endophytes could produce organic acids (73.8%) and IAA (74.3%), however, only a minor fraction (up to 0.7%) were Cd or Zn resistant or could produce siderophores or ACC deaminase. A siderophore-and ACC deaminase-producing, highly Cd-resistant Rhizobium sp. from the rhizosphere, a siderophore-, organic acid-, IAA- and ACC deaminase-producing highly Cd-resistant Pseudomonas sp. colonising the roots, a highly Cd- and Zn-resistant organic acid and IAA-producing Clavibacter sp. present in the stem, and a consortium composed of these three strains were inoculated into non-exposed and Cd-exposed yellow lupine plants. Although all selected strains possessed promising in vitro characteristics to improve Cd phytoextraction, inoculation of none of the strains (i) reduced Cd phytotoxicity nor (ii) strongly affected plant Cd uptake. This work highlights that in vitro characterisation of bacteria is not sufficient to predict the in vivo behaviour of bacteria in interaction with their host plants. This work has been financially supported by the UHasselt Methusalem project 08M03VGRJ and the European Commission under the Seventh Framework Programme for Research (FP7-KBBE-266124, GREENLAND). ... Article in Journal/Newspaper Greenland Document Server@UHasselt (Hasselt University) Greenland Plant Biology 16 5 988 996 |
institution |
Open Polar |
collection |
Document Server@UHasselt (Hasselt University) |
op_collection_id |
ftunivhasselt |
language |
English |
topic |
Cadmium natural selection phytoremediation plant-associated bacteria plant-bacteria interactions plant–bacteria interactions |
spellingShingle |
Cadmium natural selection phytoremediation plant-associated bacteria plant-bacteria interactions plant–bacteria interactions WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
topic_facet |
Cadmium natural selection phytoremediation plant-associated bacteria plant-bacteria interactions plant–bacteria interactions |
description |
In order to stimulate selection for plant-associated bacteria with the potential to improve Cd phytoextraction, yellow lupine plants were grown on a metal-contaminated field soil. It was hypothesised that growing these plants on this contaminated soil, which is a source of bacteria possessing different traits to cope with Cd, could enhance colonisation of lupine with potential plant-associated bacteria that could then be inoculated in Cd-exposed plants to reduce Cd phytotoxicity and enhance Cd uptake. All cultivable bacteria from rhizosphere, root and stem were isolated and genotypically and phenotypically characterised. Many of the rhizobacteria and root endophytes produce siderophores, organic acids, indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylate (ACC) deaminase, as well as being resistant to Cd and Zn. Most of the stem endophytes could produce organic acids (73.8%) and IAA (74.3%), however, only a minor fraction (up to 0.7%) were Cd or Zn resistant or could produce siderophores or ACC deaminase. A siderophore-and ACC deaminase-producing, highly Cd-resistant Rhizobium sp. from the rhizosphere, a siderophore-, organic acid-, IAA- and ACC deaminase-producing highly Cd-resistant Pseudomonas sp. colonising the roots, a highly Cd- and Zn-resistant organic acid and IAA-producing Clavibacter sp. present in the stem, and a consortium composed of these three strains were inoculated into non-exposed and Cd-exposed yellow lupine plants. Although all selected strains possessed promising in vitro characteristics to improve Cd phytoextraction, inoculation of none of the strains (i) reduced Cd phytotoxicity nor (ii) strongly affected plant Cd uptake. This work highlights that in vitro characterisation of bacteria is not sufficient to predict the in vivo behaviour of bacteria in interaction with their host plants. This work has been financially supported by the UHasselt Methusalem project 08M03VGRJ and the European Commission under the Seventh Framework Programme for Research (FP7-KBBE-266124, GREENLAND). ... |
author2 |
WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco |
format |
Article in Journal/Newspaper |
author |
WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco |
author_facet |
WEYENS, Nele GIELEN, Marijke BECKERS, Bram BOULET, Jana VAN DER LELIE, Daniel Taghavi, Safiyh CARLEER, Robert VANGRONSVELD, Jaco |
author_sort |
WEYENS, Nele |
title |
Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
title_short |
Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
title_full |
Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
title_fullStr |
Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
title_full_unstemmed |
Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction |
title_sort |
bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance cd phytoextraction |
publisher |
WILEY-BLACKWELL |
publishDate |
2014 |
url |
http://hdl.handle.net/1942/17764 https://doi.org/10.1111/plb.12141 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_relation |
PLANT BIOLOGY, 16 (5), p. 988-996 1435-8603 http://hdl.handle.net/1942/17764 996 5 988 16 doi:10.1111/plb.12141 000340683100016 |
op_rights |
© 2014 German Botanical Society and The Royal Botanical Society of the Netherlands. info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/plb.12141 |
container_title |
Plant Biology |
container_volume |
16 |
container_issue |
5 |
container_start_page |
988 |
op_container_end_page |
996 |
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1766020148640088064 |