THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS
We examined whether long-term Cd exposure leads to beneficial changes in the cultivable endophytic bacteria present in the seeds of Agrostis capillaris. Therefore the cultivable seed endophytes of Agrostis capillaris growing on a long-term Cd/Ni-contaminated plot (Cd/Ni seeds) were compared with tho...
Published in: | International Journal of Phytoremediation |
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Online Access: | http://hdl.handle.net/1942/16371 https://doi.org/10.1080/15226514.2013.837027 |
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ftunivhasselt:oai:documentserver.uhasselt.be:1942/16371 2023-05-15T16:29:59+02:00 THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS Truyens, Sascha Jambon, Inge Croes, Sarah Janssen, Jolien Weyens, Nele Mench, Michel Carleer, Robert Cuypers, Ann Vangronsveld, Jaco 2014 application/pdf http://hdl.handle.net/1942/16371 https://doi.org/10.1080/15226514.2013.837027 en eng INTERNATIONAL JOURNAL OF PHYTOREMEDIATION, 16 (7-8), p. 643-659 1522-6514 http://hdl.handle.net/1942/16371 659 7-8 643 16 doi:10.1080/15226514.2013.837027 000329510200012 Copyright: Taylor & Francis Group info:eu-repo/semantics/restrictedAccess agrostis capillaris metals seed endophytes phenotypic characterisation plant growth promotion info:eu-repo/semantics/article 2014 ftunivhasselt https://doi.org/10.1080/15226514.2013.837027 2022-08-11T12:30:25Z We examined whether long-term Cd exposure leads to beneficial changes in the cultivable endophytic bacteria present in the seeds of Agrostis capillaris. Therefore the cultivable seed endophytes of Agrostis capillaris growing on a long-term Cd/Ni-contaminated plot (Cd/Ni seeds) were compared with those originating from a non-contaminated plot (control seeds). We observed plant- and contaminant-dependent effects on the population composition between control and Cd/Ni seeds. Also differences in phenotypic characteristics were found: endophytes from Cd/Ni seeds exhibited more ACC deaminase activity and production of siderophores and IAA, while endophytes from control seeds, very surprisingly, showed more metal tolerance. Finally, the 3 most promising seed endophytes were selected based on their metal tolerance and plant growth promoting potential, and inoculated in Agrostis capillaris seedlings. In case of non-exposed plants, inoculation resulted in a significantly improved plant growth; after inoculation of Cd-exposed plants an increased Cd uptake was achieved without affecting plant growth. This indicates that inoculation of Agrostis with its seed endophytes might be beneficial for its establishment during phytoextraction and phytostabilisation of Cd-contaminated soils. This research was funded by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) for J.J. and by the Fund for Scientific Research Flanders (FWO-Vlaanderen), Ph.D. grant for S. C. and S. T. and post-doc grant for N.W. This work has been financially supported by the UHasselt Methusalem project 08M03VGRJ and by the European Commission under the Seventh Framework Programme for Research (FP7-KBBE-266124, GREENLAND). Article in Journal/Newspaper Greenland Document Server@UHasselt (Hasselt University) Greenland International Journal of Phytoremediation 16 7-8 643 659 |
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Open Polar |
collection |
Document Server@UHasselt (Hasselt University) |
op_collection_id |
ftunivhasselt |
language |
English |
topic |
agrostis capillaris metals seed endophytes phenotypic characterisation plant growth promotion |
spellingShingle |
agrostis capillaris metals seed endophytes phenotypic characterisation plant growth promotion Truyens, Sascha Jambon, Inge Croes, Sarah Janssen, Jolien Weyens, Nele Mench, Michel Carleer, Robert Cuypers, Ann Vangronsveld, Jaco THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
topic_facet |
agrostis capillaris metals seed endophytes phenotypic characterisation plant growth promotion |
description |
We examined whether long-term Cd exposure leads to beneficial changes in the cultivable endophytic bacteria present in the seeds of Agrostis capillaris. Therefore the cultivable seed endophytes of Agrostis capillaris growing on a long-term Cd/Ni-contaminated plot (Cd/Ni seeds) were compared with those originating from a non-contaminated plot (control seeds). We observed plant- and contaminant-dependent effects on the population composition between control and Cd/Ni seeds. Also differences in phenotypic characteristics were found: endophytes from Cd/Ni seeds exhibited more ACC deaminase activity and production of siderophores and IAA, while endophytes from control seeds, very surprisingly, showed more metal tolerance. Finally, the 3 most promising seed endophytes were selected based on their metal tolerance and plant growth promoting potential, and inoculated in Agrostis capillaris seedlings. In case of non-exposed plants, inoculation resulted in a significantly improved plant growth; after inoculation of Cd-exposed plants an increased Cd uptake was achieved without affecting plant growth. This indicates that inoculation of Agrostis with its seed endophytes might be beneficial for its establishment during phytoextraction and phytostabilisation of Cd-contaminated soils. This research was funded by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) for J.J. and by the Fund for Scientific Research Flanders (FWO-Vlaanderen), Ph.D. grant for S. C. and S. T. and post-doc grant for N.W. This work has been financially supported by the UHasselt Methusalem project 08M03VGRJ and by the European Commission under the Seventh Framework Programme for Research (FP7-KBBE-266124, GREENLAND). |
format |
Article in Journal/Newspaper |
author |
Truyens, Sascha Jambon, Inge Croes, Sarah Janssen, Jolien Weyens, Nele Mench, Michel Carleer, Robert Cuypers, Ann Vangronsveld, Jaco |
author_facet |
Truyens, Sascha Jambon, Inge Croes, Sarah Janssen, Jolien Weyens, Nele Mench, Michel Carleer, Robert Cuypers, Ann Vangronsveld, Jaco |
author_sort |
Truyens, Sascha |
title |
THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
title_short |
THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
title_full |
THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
title_fullStr |
THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
title_full_unstemmed |
THE EFFECT OF LONG-TERM CD AND NI EXPOSURE ON SEED ENDOPHYTES OF AGROSTIS CAPILLARIS AND THEIR POTENTIAL APPLICATION IN PHYTOREMEDIATION OF METAL-CONTAMINATED SOILS |
title_sort |
effect of long-term cd and ni exposure on seed endophytes of agrostis capillaris and their potential application in phytoremediation of metal-contaminated soils |
publishDate |
2014 |
url |
http://hdl.handle.net/1942/16371 https://doi.org/10.1080/15226514.2013.837027 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_relation |
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION, 16 (7-8), p. 643-659 1522-6514 http://hdl.handle.net/1942/16371 659 7-8 643 16 doi:10.1080/15226514.2013.837027 000329510200012 |
op_rights |
Copyright: Taylor & Francis Group info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1080/15226514.2013.837027 |
container_title |
International Journal of Phytoremediation |
container_volume |
16 |
container_issue |
7-8 |
container_start_page |
643 |
op_container_end_page |
659 |
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1766019687001358336 |