Evolutionary analysis and genetic mapping of neuropeptide Y (NPY) receptors in rainbow trout(Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus)

• Main Author: Goldt, Aaron
• Other Authors: Danzmann, Roy, Ferguson, Moira
• Format: Thesis
• Language: English
• Published: University of Guelph 2012
• Subjects: rainbow trout (Oncorhynchus mykiss); Arctic charr (Salvelinus alpinus); gene duplication; neuropeptide Y (NPY); Arctic; Canada; Arctic charr; Salvelinus alpinus
• Online Access: http://hdl.handle.net/10214/4048

Neuropeptide Y (NPY) receptors are an important multifunctional gene family found in vertebrates characterized by a complex evolutionary history of gene duplication and loss. I genetically mapped and sequenced the NPY receptors in rainbow trout (Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus) to examine the fate of this receptor family following the whole genome duplication in the salmonid ancestor. Based on receptors present in model teleosts, the Y1, Y2, Y4, Y7, Y8a and Y8b receptors were predicted to occur in the salmonid genome. Single copies of Y2, Y7 and Y8b and a tandem duplication of Y8a were detected in both rainbow trout and Arctic charr. Bayesian phylogenetic analysis strongly supports the model of NPY receptor evolution through multiple duplication events. The absence of multiple receptor subtypes and the presence of a tandem duplicate were unexpected but fall within patterns observed from previous duplication events. Natural Sciences and Engineering Research Council of Canada