Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. The accuracy of bacterial identification using MALDI-TOF MS depends on main spectral profiles (MSPs) provided in a quality-assured commercial refere...
Published in: | Frontiers in Veterinary Science |
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Online Access: | https://hdl.handle.net/10214/28299 https://doi.org/10.3389/fvets.2022.1031373 |
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ftunivguelph:oai:atrium.lib.uoguelph.ca:10214/28299 2024-06-09T07:44:40+00:00 Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Ojasanya, Rasaq Gardner, Ian Groman, David Saksida, Sonja Saab, Matthew Thakur, Krishna 2022-10-20 pdf application/pdf https://hdl.handle.net/10214/28299 https://doi.org/10.3389/fvets.2022.1031373 unknown Frontiers Media SA Frontiers in Veterinary Science Ojasanya, R. A., Gardner, I. A., Groman, D., Saksida, S., Saab, M. E., & Thakur, K. K. (2022, October 20). Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Frontiers in Veterinary Science. Frontiers Media SA. http://doi.org/10.3389/fvets.2022.1031373 https://hdl.handle.net/10214/28299 doi:10.3389/fvets.2022.1031373 Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ MALDI-TOF MS main spectral profile Yersinia ruckeri aquaculture validation Article 2022 ftunivguelph https://doi.org/10.3389/fvets.2022.1031373 2024-05-14T23:30:36Z Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. The accuracy of bacterial identification using MALDI-TOF MS depends on main spectral profiles (MSPs) provided in a quality-assured commercial reference library, which requires ongoing improvement. This study aimed to develop and validate an in-house MALDI-TOF MS MSP to rapidly identify Yersinia ruckeri isolated from Atlantic salmon ( Salmo salar ). The novel MSP was prepared using an isolate of Y. ruckeri recovered from Atlantic salmon and confirmed by 16S rRNA gene sequencing. Subsequently, a validation set which comprises 29 isolates of Y. ruckeri were examined from three fishes: Atlantic salmon ( Salmo salar ) ( n = 26), American eel ( Anguilla rostrata ) ( n = 1), and Atlantic cod ( Gadus morhua ) ( n = 2). These isolates were randomly selected from the Atlantic Veterinary College, Aquatic Diagnostic Services Bacteriology Laboratory's culture collection to validate the novel MSP. Analytical sensitivity of MALDI-TOF MS using the novel MSP to identify the validation set was 86.2%. Repeatability was assessed by acquiring spectra from 30 different spots of a randomly-selected isolate of Y. ruckeri , and analyzed spectra from each spot were compared against the novel MSP. The coefficient of variation was 3.3%. The novel MSP clustered with Bruker MSPs ( n = 3) of Y. ruckeri in the reference library and did not falsely identify any closely related bacteria to Y. ruckeri . This study reports the development of a novel MSP of high analytical sensitivity and specificity for rapid identification of Y. ruckeri using MALDI-TOF MS. Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Salmo salar University of Guelph: DSpace digital archive Frontiers in Veterinary Science 9 |
institution |
Open Polar |
collection |
University of Guelph: DSpace digital archive |
op_collection_id |
ftunivguelph |
language |
unknown |
topic |
MALDI-TOF MS main spectral profile Yersinia ruckeri aquaculture validation |
spellingShingle |
MALDI-TOF MS main spectral profile Yersinia ruckeri aquaculture validation Ojasanya, Rasaq Gardner, Ian Groman, David Saksida, Sonja Saab, Matthew Thakur, Krishna Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
topic_facet |
MALDI-TOF MS main spectral profile Yersinia ruckeri aquaculture validation |
description |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. The accuracy of bacterial identification using MALDI-TOF MS depends on main spectral profiles (MSPs) provided in a quality-assured commercial reference library, which requires ongoing improvement. This study aimed to develop and validate an in-house MALDI-TOF MS MSP to rapidly identify Yersinia ruckeri isolated from Atlantic salmon ( Salmo salar ). The novel MSP was prepared using an isolate of Y. ruckeri recovered from Atlantic salmon and confirmed by 16S rRNA gene sequencing. Subsequently, a validation set which comprises 29 isolates of Y. ruckeri were examined from three fishes: Atlantic salmon ( Salmo salar ) ( n = 26), American eel ( Anguilla rostrata ) ( n = 1), and Atlantic cod ( Gadus morhua ) ( n = 2). These isolates were randomly selected from the Atlantic Veterinary College, Aquatic Diagnostic Services Bacteriology Laboratory's culture collection to validate the novel MSP. Analytical sensitivity of MALDI-TOF MS using the novel MSP to identify the validation set was 86.2%. Repeatability was assessed by acquiring spectra from 30 different spots of a randomly-selected isolate of Y. ruckeri , and analyzed spectra from each spot were compared against the novel MSP. The coefficient of variation was 3.3%. The novel MSP clustered with Bruker MSPs ( n = 3) of Y. ruckeri in the reference library and did not falsely identify any closely related bacteria to Y. ruckeri . This study reports the development of a novel MSP of high analytical sensitivity and specificity for rapid identification of Y. ruckeri using MALDI-TOF MS. |
format |
Article in Journal/Newspaper |
author |
Ojasanya, Rasaq Gardner, Ian Groman, David Saksida, Sonja Saab, Matthew Thakur, Krishna |
author_facet |
Ojasanya, Rasaq Gardner, Ian Groman, David Saksida, Sonja Saab, Matthew Thakur, Krishna |
author_sort |
Ojasanya, Rasaq |
title |
Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_short |
Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full |
Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_fullStr |
Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full_unstemmed |
Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_sort |
development and validation of main spectral profile for rapid identification of yersinia ruckeri isolated from atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
https://hdl.handle.net/10214/28299 https://doi.org/10.3389/fvets.2022.1031373 |
genre |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
op_relation |
Frontiers in Veterinary Science Ojasanya, R. A., Gardner, I. A., Groman, D., Saksida, S., Saab, M. E., & Thakur, K. K. (2022, October 20). Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Frontiers in Veterinary Science. Frontiers Media SA. http://doi.org/10.3389/fvets.2022.1031373 https://hdl.handle.net/10214/28299 doi:10.3389/fvets.2022.1031373 |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fvets.2022.1031373 |
container_title |
Frontiers in Veterinary Science |
container_volume |
9 |
_version_ |
1801373463741464576 |