Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos
Studies to date have shown that wood bison embryo development in vitro is compromised with little or no embryos developing to the blastocyst stage. The aim of this study was to use bison-cattle hybrid embryos, an interspecies that is known to result in live offspring in vivo, as a model for assessin...
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University of Guelph
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ftunivguelph:oai:atrium.lib.uoguelph.ca:10214/25874 2023-11-05T03:45:25+01:00 Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos Seaby, Richard Peter Mastromonaco, G.F. 2010 application/pdf https://hdl.handle.net/10214/25874 en eng University of Guelph https://hdl.handle.net/10214/25874 All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. Wood bison Plains Bison hybrid embryos developmental potential nulcear/mitochondrial interactions Thesis 2010 ftunivguelph 2023-10-08T06:15:42Z Studies to date have shown that wood bison embryo development in vitro is compromised with little or no embryos developing to the blastocyst stage. The aim of this study was to use bison-cattle hybrid embryos, an interspecies that is known to result in live offspring in vivo, as a model for assessing species-specific differences in embryo development in vitro. Cattle oocytes were fertilized with cattle, plains bison and wood bison sperm. Blastocyst development rates were 34.45 ± 2.45% (cattle), 39.56 ± 3.44% (plains bison hybrid), 23.43 ± 1.33% (wood bison hybrid). Plains and wood bison hybrid blastocysts had significantly lower cell numbers than cattle blastocysts (110.1 ± 4.6, 104.2 ± 5.9, 133.3 ± 8.3, respectively; n=10). There was significantly more apoptosis in hybrid wood bison blastocysts than in those of either cattle or hybrid plains bison. As well, ATP levels (n=25) and expression profiles of NRF1, mtTFA and Cytb (n=3, rep=3) were not significantly different among groups in both 8-16 cell and blastocyst stage embryos. These data confirm that wood bison hybrid embryos are incompetent compared to cattle embryos most likely due to media conditions. Thesis Wood Bison Plains Bison University of Guelph: DSpace digital archive |
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Open Polar |
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University of Guelph: DSpace digital archive |
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ftunivguelph |
language |
English |
topic |
Wood bison Plains Bison hybrid embryos developmental potential nulcear/mitochondrial interactions |
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Wood bison Plains Bison hybrid embryos developmental potential nulcear/mitochondrial interactions Seaby, Richard Peter Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
topic_facet |
Wood bison Plains Bison hybrid embryos developmental potential nulcear/mitochondrial interactions |
description |
Studies to date have shown that wood bison embryo development in vitro is compromised with little or no embryos developing to the blastocyst stage. The aim of this study was to use bison-cattle hybrid embryos, an interspecies that is known to result in live offspring in vivo, as a model for assessing species-specific differences in embryo development in vitro. Cattle oocytes were fertilized with cattle, plains bison and wood bison sperm. Blastocyst development rates were 34.45 ± 2.45% (cattle), 39.56 ± 3.44% (plains bison hybrid), 23.43 ± 1.33% (wood bison hybrid). Plains and wood bison hybrid blastocysts had significantly lower cell numbers than cattle blastocysts (110.1 ± 4.6, 104.2 ± 5.9, 133.3 ± 8.3, respectively; n=10). There was significantly more apoptosis in hybrid wood bison blastocysts than in those of either cattle or hybrid plains bison. As well, ATP levels (n=25) and expression profiles of NRF1, mtTFA and Cytb (n=3, rep=3) were not significantly different among groups in both 8-16 cell and blastocyst stage embryos. These data confirm that wood bison hybrid embryos are incompetent compared to cattle embryos most likely due to media conditions. |
author2 |
Mastromonaco, G.F. |
format |
Thesis |
author |
Seaby, Richard Peter |
author_facet |
Seaby, Richard Peter |
author_sort |
Seaby, Richard Peter |
title |
Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
title_short |
Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
title_full |
Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
title_fullStr |
Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
title_full_unstemmed |
Investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
title_sort |
investigation of developmental potential and nuclear/mitochondrial interactions in early wood bison and plains bison hybrid embryos |
publisher |
University of Guelph |
publishDate |
2010 |
url |
https://hdl.handle.net/10214/25874 |
genre |
Wood Bison Plains Bison |
genre_facet |
Wood Bison Plains Bison |
op_relation |
https://hdl.handle.net/10214/25874 |
op_rights |
All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. |
_version_ |
1781707795701170176 |