Molecular genetic analysis of an Atlantic salmon (Salmo salar) broodstock: Pedigree analysis and QTL detection for body weight

• Main Author: Szanto, Annamaria
• Other Authors: Ferguson, M.M., Danzmann, R.G.
• Format: Thesis
• Language: English
• Published: University of Guelph 2002
• Subjects: multiplex genotyping system; Atlantic salmon; Salmo salar; Atlantic Salmon Broodstock Development Program; Chamcook; New Brunswick
• Online Access: https://hdl.handle.net/10214/25653

A multiplex genotyping system was applied to Atlantic salmon (' Salmo salar') being reared by the Atlantic Salmon Broodstock Development Program in Chamcook, New Brunswick. Nine microsatellite loci were used to determine the parents of 98% of 305 progeny from the 1998 year-class reared in a sea cage. The genotyping system validated family identity of 299 fish, which had been initially identified by branding. The genotyping system was also able to discriminate among all but 5 of 79 families in the 1998 year-class when progeny genotypes were generated from genotypes of known parental pairs. In a second experiment, two quantitative trait loci for body weight were identified, using progeny from two full-sib families of the 1998 year-class. Out of 25 microsatellite loci tested, association between variation and body weight was detected at two of these microsatellite loci. The QTL explained between 9.6 and 14% of the variation in body weight.