Apolipoprotein domains and innate immunity in rainbow trout and walleye

• Main Author: Di Natale, Adrian
• Other Authors: Lumsden, John
• Format: Thesis
• Language: English
• Published: University of Guelph 2017
• Subjects: rainbow trout; ice structuring protein; acute phase response; Flavobacterium psychrophilum; qPCR; walleye; apolipoprotein; Atlantic salmon; Salmo salar
• Online Access: http://hdl.handle.net/10214/11523

An unidentified rainbow trout LPS-inducible protein “p9.5” was characterized using RACE PCR, ELISA, SDS-PAGE and Western blot. RACE PCR generated a sequence 98.6% similar to type-IV ice-structuring protein LS-12 in Atlantic salmon (Salmo Salar). Gene expression and plasma protein concentration were quantified following experimental infection of rainbow trout with F. psychrophilum. LS-12 plasma concentration did not change up to 15 d post infection (pi), while hepatic gene expression was significantly down-regulated at 3 and 6 d pi. Walleye pattern recognition receptors (PRRs) were investigated using a plasma-binding assay to killed F. columnare. Partial amino acid sequence from a 22 kDa plasma protein was used to develop PCR primers that generated a 159 bp nucleotide sequence that was 72.4% similar to apolipoprotein A-I from striped bass (Morone saxatilis). RT-qPCR quantified hepatic gene expression following experimental infection of walleye with viral haemorrhage septicaemia virus. Apolipoprotein A-I was significantly upregulated in the livers of walleye at 6 and 11 d pi.