Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding
This thesis investigates the use of DNA metabarcoding as a tool for assessing biodiversity patterns in freshwater macroinvertebrate communities within the Peace-Athabasca Delta. Macroinvertebrate samples were collected in June and August of 2012 and 2013 from both the Peace and Athabasca River delta...
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University of Guelph
2017
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ftunivguelph:oai:atrium.lib.uoguelph.ca:10214/10186 2024-06-23T07:51:00+00:00 Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding Wright, Michael TG Hajibabaei, Mehrdad 2017-01-06 application/pdf application/vnd.openxmlformats-officedocument.spreadsheetml.sheet http://hdl.handle.net/10214/10186 en eng University of Guelph http://hdl.handle.net/10214/10186 Attribution-ShareAlike 2.5 Canada http://creativecommons.org/licenses/by-sa/2.5/ca/ DNA metabarcoding wetland macroinvertebrate Thesis 2017 ftunivguelph 2024-06-04T23:58:45Z This thesis investigates the use of DNA metabarcoding as a tool for assessing biodiversity patterns in freshwater macroinvertebrate communities within the Peace-Athabasca Delta. Macroinvertebrate samples were collected in June and August of 2012 and 2013 from both the Peace and Athabasca River deltas in northern Alberta. Following the morphological identification of a subset of individuals within the samples, macroinvertebrates were homogenized, bulk DNA was extracted, and two fragments of the mitochondrial Cytochrome c oxidase I (COI) gene were sequenced and compared against two reference libraries obtained 18 months apart. When comparing taxa identified via DNA metabarcoding to those identified through morphology, 73% of the total occurrences were the same. This number increased to 84% through passive additions to online genomic repositories over the course of this study, emphasizing the importance of reference library coverage in metabarcoding analysis. Despite some taxa known to be present in the samples by morphological identification not being identified by DNA metabarcoding, general diversity patterns were consistent between the two methods of identification. The river delta of origin was the primary factor separating macroinvertebrate communities, followed by season of sampling. Thesis Athabasca River University of Guelph: DSpace digital archive Athabasca River Peace-Athabasca Delta ENVELOPE(-111.502,-111.502,58.667,58.667) |
institution |
Open Polar |
collection |
University of Guelph: DSpace digital archive |
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ftunivguelph |
language |
English |
topic |
DNA metabarcoding wetland macroinvertebrate |
spellingShingle |
DNA metabarcoding wetland macroinvertebrate Wright, Michael TG Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
topic_facet |
DNA metabarcoding wetland macroinvertebrate |
description |
This thesis investigates the use of DNA metabarcoding as a tool for assessing biodiversity patterns in freshwater macroinvertebrate communities within the Peace-Athabasca Delta. Macroinvertebrate samples were collected in June and August of 2012 and 2013 from both the Peace and Athabasca River deltas in northern Alberta. Following the morphological identification of a subset of individuals within the samples, macroinvertebrates were homogenized, bulk DNA was extracted, and two fragments of the mitochondrial Cytochrome c oxidase I (COI) gene were sequenced and compared against two reference libraries obtained 18 months apart. When comparing taxa identified via DNA metabarcoding to those identified through morphology, 73% of the total occurrences were the same. This number increased to 84% through passive additions to online genomic repositories over the course of this study, emphasizing the importance of reference library coverage in metabarcoding analysis. Despite some taxa known to be present in the samples by morphological identification not being identified by DNA metabarcoding, general diversity patterns were consistent between the two methods of identification. The river delta of origin was the primary factor separating macroinvertebrate communities, followed by season of sampling. |
author2 |
Hajibabaei, Mehrdad |
format |
Thesis |
author |
Wright, Michael TG |
author_facet |
Wright, Michael TG |
author_sort |
Wright, Michael TG |
title |
Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
title_short |
Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
title_full |
Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
title_fullStr |
Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
title_full_unstemmed |
Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding |
title_sort |
assessing temporal changes in macroinvertebrate community structure in a remote wetland through dna metabarcoding |
publisher |
University of Guelph |
publishDate |
2017 |
url |
http://hdl.handle.net/10214/10186 |
long_lat |
ENVELOPE(-111.502,-111.502,58.667,58.667) |
geographic |
Athabasca River Peace-Athabasca Delta |
geographic_facet |
Athabasca River Peace-Athabasca Delta |
genre |
Athabasca River |
genre_facet |
Athabasca River |
op_relation |
http://hdl.handle.net/10214/10186 |
op_rights |
Attribution-ShareAlike 2.5 Canada http://creativecommons.org/licenses/by-sa/2.5/ca/ |
_version_ |
1802641999941074944 |