Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel
Hormones and mRNA transcripts of maternal origin deposited in the egg may affect early embryonic development in oviparous species. These hormones include steroids, such as estradiol-17β (E2), testosterone (T), 11-ketotestosterone (11-kt), 17α,20ß-dihydroxy-4-pregnen-3-one (DHP), and cortisol, which...
Published in: | General and Comparative Endocrinology |
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Language: | English |
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Elsevier
2021
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Online Access: | http://gala.gre.ac.uk/id/eprint/33360/ http://gala.gre.ac.uk/id/eprint/33360/1/33647_GRIFFITHS_Charge%20Modification%20as%20a%20Mechanism.pdf https://doi.org/10.1016/j.ygcen.2021.113854 |
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ftunivgreenwich:oai:gala.gre.ac.uk:33360 2023-05-15T13:28:17+02:00 Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel Kottmann, Johanna S. Tveiten, Helge Miest, Joanna Junack Tomkiewicz, Jonna 2021-09-15 application/pdf http://gala.gre.ac.uk/id/eprint/33360/ http://gala.gre.ac.uk/id/eprint/33360/1/33647_GRIFFITHS_Charge%20Modification%20as%20a%20Mechanism.pdf https://doi.org/10.1016/j.ygcen.2021.113854 en eng Elsevier http://gala.gre.ac.uk/id/eprint/33360/1/33647_GRIFFITHS_Charge%20Modification%20as%20a%20Mechanism.pdf Kottmann, Johanna S., Tveiten, Helge, Miest, Joanna Junack orcid:0000-0003-2534-0530 and Tomkiewicz, Jonna (2021) Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel. General and Comparative Endocrinology, 311:113854. ISSN 0016-6480 (doi:https://doi.org/10.1016/j.ygcen.2021.113854 <https://doi.org/10.1016/j.ygcen.2021.113854>) cc_by_nc_nd_4 SH Aquaculture. Fisheries. Angling Article PeerReviewed 2021 ftunivgreenwich https://doi.org/10.1016/j.ygcen.2021.113854 2023-03-26T20:31:26Z Hormones and mRNA transcripts of maternal origin deposited in the egg may affect early embryonic development in oviparous species. These hormones include steroids, such as estradiol-17β (E2), testosterone (T), 11-ketotestosterone (11-kt), 17α,20ß-dihydroxy-4-pregnen-3-one (DHP), and cortisol, which also play an important role in fish reproduction. In European eel, Anguilla anguilla, which does not reproduce naturally in captivity, vitellogenesis in female broodstock is commonly induced by administration of salmon or carp pituitary extract (PE) as an exogenous source of gonadotropins, while follicular maturation is stimulated by a priming dose of PE followed by provision of DHP as a maturation inducing hormone. In this regard, the main purpose of the present study was to evaluate effects of induced follicular maturation on reproductive success in European eel, focusing on maternal transfer and dynamics of steroids and mRNA transcripts of growth- and development-related genes throughout embryogenesis. The results showed that maternal blood plasma concentrations of E2, T and DHP were reflected in the unfertilized eggs. Moreover, a negative relationship between concentrations of E2 and DHP in eggs and embryos and quality parameters measured as fertilization success, cleavage abnormalities, embryonic survival, and hatch success was found. Concomitant mRNA transcript abundance analysis including genes involved in stress response (hsp70, hsp90), somatotropic axis (gh, igf1, igf2a, igf2b), lipid (cpt1a, cpt1b, pigf5) and thyroid metabolism (dio1, dio2, dio3, thrαb, thrβa, thrβb) varied among unfertilized egg batches. For the majority of genes, mRNA abundance increased during the maternal-to-zygotic transition in connection to activation of the transcription of the embryos own genome. mRNA abundance of dio1, cpt1a and cpt1b throughout embryogenesis was related to embryonic developmental competence. Notably, mRNA abundance of dio3 was positively associated with E2 concentrations, while the mRNA abundance of thrαb was ... Article in Journal/Newspaper Anguilla anguilla European eel University of Greenwich: Greenwich Academic Literature Archive General and Comparative Endocrinology 311 113854 |
institution |
Open Polar |
collection |
University of Greenwich: Greenwich Academic Literature Archive |
op_collection_id |
ftunivgreenwich |
language |
English |
topic |
SH Aquaculture. Fisheries. Angling |
spellingShingle |
SH Aquaculture. Fisheries. Angling Kottmann, Johanna S. Tveiten, Helge Miest, Joanna Junack Tomkiewicz, Jonna Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
topic_facet |
SH Aquaculture. Fisheries. Angling |
description |
Hormones and mRNA transcripts of maternal origin deposited in the egg may affect early embryonic development in oviparous species. These hormones include steroids, such as estradiol-17β (E2), testosterone (T), 11-ketotestosterone (11-kt), 17α,20ß-dihydroxy-4-pregnen-3-one (DHP), and cortisol, which also play an important role in fish reproduction. In European eel, Anguilla anguilla, which does not reproduce naturally in captivity, vitellogenesis in female broodstock is commonly induced by administration of salmon or carp pituitary extract (PE) as an exogenous source of gonadotropins, while follicular maturation is stimulated by a priming dose of PE followed by provision of DHP as a maturation inducing hormone. In this regard, the main purpose of the present study was to evaluate effects of induced follicular maturation on reproductive success in European eel, focusing on maternal transfer and dynamics of steroids and mRNA transcripts of growth- and development-related genes throughout embryogenesis. The results showed that maternal blood plasma concentrations of E2, T and DHP were reflected in the unfertilized eggs. Moreover, a negative relationship between concentrations of E2 and DHP in eggs and embryos and quality parameters measured as fertilization success, cleavage abnormalities, embryonic survival, and hatch success was found. Concomitant mRNA transcript abundance analysis including genes involved in stress response (hsp70, hsp90), somatotropic axis (gh, igf1, igf2a, igf2b), lipid (cpt1a, cpt1b, pigf5) and thyroid metabolism (dio1, dio2, dio3, thrαb, thrβa, thrβb) varied among unfertilized egg batches. For the majority of genes, mRNA abundance increased during the maternal-to-zygotic transition in connection to activation of the transcription of the embryos own genome. mRNA abundance of dio1, cpt1a and cpt1b throughout embryogenesis was related to embryonic developmental competence. Notably, mRNA abundance of dio3 was positively associated with E2 concentrations, while the mRNA abundance of thrαb was ... |
format |
Article in Journal/Newspaper |
author |
Kottmann, Johanna S. Tveiten, Helge Miest, Joanna Junack Tomkiewicz, Jonna |
author_facet |
Kottmann, Johanna S. Tveiten, Helge Miest, Joanna Junack Tomkiewicz, Jonna |
author_sort |
Kottmann, Johanna S. |
title |
Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
title_short |
Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
title_full |
Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
title_fullStr |
Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
title_full_unstemmed |
Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel |
title_sort |
sex steroid dynamics and mrna transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in european eel |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://gala.gre.ac.uk/id/eprint/33360/ http://gala.gre.ac.uk/id/eprint/33360/1/33647_GRIFFITHS_Charge%20Modification%20as%20a%20Mechanism.pdf https://doi.org/10.1016/j.ygcen.2021.113854 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
http://gala.gre.ac.uk/id/eprint/33360/1/33647_GRIFFITHS_Charge%20Modification%20as%20a%20Mechanism.pdf Kottmann, Johanna S., Tveiten, Helge, Miest, Joanna Junack orcid:0000-0003-2534-0530 and Tomkiewicz, Jonna (2021) Sex steroid dynamics and mRNA transcript profiles of growth- and development-related genes during embryogenesis following induced follicular maturation in European eel. General and Comparative Endocrinology, 311:113854. ISSN 0016-6480 (doi:https://doi.org/10.1016/j.ygcen.2021.113854 <https://doi.org/10.1016/j.ygcen.2021.113854>) |
op_rights |
cc_by_nc_nd_4 |
op_doi |
https://doi.org/10.1016/j.ygcen.2021.113854 |
container_title |
General and Comparative Endocrinology |
container_volume |
311 |
container_start_page |
113854 |
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1766403217276534784 |