Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae
The honey locust gall midge, Dasineura gleditchiae Osten Sacken 1866 (Diptera: Cecidomyiidae) is the main pest of ornamental varieties of the honey locust tree, Gleditsia triacanthos L., in North America, and is now becoming a pest of concern in Europe. Female midges were observed to emerge in the e...
Published in: | Journal of Chemical Ecology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Springer-Verlag
2009
|
Subjects: | |
Online Access: | http://gala.gre.ac.uk/id/eprint/2060/ https://doi.org/10.1007/s10886-009-9641-5 |
id |
ftunivgreenwich:oai:gala.gre.ac.uk:2060 |
---|---|
record_format |
openpolar |
spelling |
ftunivgreenwich:oai:gala.gre.ac.uk:2060 2023-05-15T13:55:11+02:00 Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae Molnár, Béla Kárpáti, Zsolt Szőcs, Gábor Hall, David R. 2009-05-21 http://gala.gre.ac.uk/id/eprint/2060/ https://doi.org/10.1007/s10886-009-9641-5 unknown Springer-Verlag Molnár, Béla, Kárpáti, Zsolt, Szőcs, Gábor and Hall, David R. orcid:0000-0002-7887-466X (2009) Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae. Journal of Chemical Ecology, 35 (6). pp. 706-714. ISSN 0098-0331 (Print), 1573-1561 (Online) (doi:https://doi.org/10.1007/s10886-009-9641-5 <https://doi.org/10.1007/s10886-009-9641-5>) S Agriculture (General) SF Animal culture SB Plant culture Article PeerReviewed 2009 ftunivgreenwich https://doi.org/10.1007/s10886-009-9641-5 2023-03-26T20:21:31Z The honey locust gall midge, Dasineura gleditchiae Osten Sacken 1866 (Diptera: Cecidomyiidae) is the main pest of ornamental varieties of the honey locust tree, Gleditsia triacanthos L., in North America, and is now becoming a pest of concern in Europe. Female midges were observed to emerge in the early morning with their ovipositor extended until they mated. Volatiles were collected from virgin females in a closed-loop stripping apparatus and analyzed by gas chromatography (GC) coupled to electroantennographic (EAG) recording from the antenna of a male midge. A single EAG response was observed, which was assumed to be to the major component of the female sex pheromone. This was identified as (Z)-2-acetoxy-8-heptadecene by comparison of its mass spectrum and GC retention times on different columns with those of synthetic standards and by micro-analytical reactions. This compound was synthesized, and the individual enantiomers were produced by kinetic resolution with lipase from Candida antarctica. Analysis of the naturally-produced compound on a cyclodextrin GC column indicated it was the (R)-enantiomer. In EAG dose-response measurements, the (R)-enantiomer alone or in the racemic mixture evoked significant responses from the antennae of male D. gleditchiae, whereas the (S)-enantiomer did not. In field trapping tests, the (R)-enantiomer attracted male D. gleditchiae. The racemic compound was equally attractive, but the (S)-enantiomer was not attractive. Both the pure (R)-enantiomer or racemic (Z)-2-acetoxy-8-heptadecene, applied to red rubber septa in a dose range of 3–30 μg, constitute a strongly attractive bait in sticky traps for monitoring the flight of D. gleditchiae. Article in Journal/Newspaper Antarc* Antarctica University of Greenwich: Greenwich Academic Literature Archive Journal of Chemical Ecology 35 6 706 714 |
institution |
Open Polar |
collection |
University of Greenwich: Greenwich Academic Literature Archive |
op_collection_id |
ftunivgreenwich |
language |
unknown |
topic |
S Agriculture (General) SF Animal culture SB Plant culture |
spellingShingle |
S Agriculture (General) SF Animal culture SB Plant culture Molnár, Béla Kárpáti, Zsolt Szőcs, Gábor Hall, David R. Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
topic_facet |
S Agriculture (General) SF Animal culture SB Plant culture |
description |
The honey locust gall midge, Dasineura gleditchiae Osten Sacken 1866 (Diptera: Cecidomyiidae) is the main pest of ornamental varieties of the honey locust tree, Gleditsia triacanthos L., in North America, and is now becoming a pest of concern in Europe. Female midges were observed to emerge in the early morning with their ovipositor extended until they mated. Volatiles were collected from virgin females in a closed-loop stripping apparatus and analyzed by gas chromatography (GC) coupled to electroantennographic (EAG) recording from the antenna of a male midge. A single EAG response was observed, which was assumed to be to the major component of the female sex pheromone. This was identified as (Z)-2-acetoxy-8-heptadecene by comparison of its mass spectrum and GC retention times on different columns with those of synthetic standards and by micro-analytical reactions. This compound was synthesized, and the individual enantiomers were produced by kinetic resolution with lipase from Candida antarctica. Analysis of the naturally-produced compound on a cyclodextrin GC column indicated it was the (R)-enantiomer. In EAG dose-response measurements, the (R)-enantiomer alone or in the racemic mixture evoked significant responses from the antennae of male D. gleditchiae, whereas the (S)-enantiomer did not. In field trapping tests, the (R)-enantiomer attracted male D. gleditchiae. The racemic compound was equally attractive, but the (S)-enantiomer was not attractive. Both the pure (R)-enantiomer or racemic (Z)-2-acetoxy-8-heptadecene, applied to red rubber septa in a dose range of 3–30 μg, constitute a strongly attractive bait in sticky traps for monitoring the flight of D. gleditchiae. |
format |
Article in Journal/Newspaper |
author |
Molnár, Béla Kárpáti, Zsolt Szőcs, Gábor Hall, David R. |
author_facet |
Molnár, Béla Kárpáti, Zsolt Szőcs, Gábor Hall, David R. |
author_sort |
Molnár, Béla |
title |
Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
title_short |
Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
title_full |
Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
title_fullStr |
Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
title_full_unstemmed |
Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae |
title_sort |
identification of female-produced sex pheromone of the honey locust gall midge, dasineura gleditchiae |
publisher |
Springer-Verlag |
publishDate |
2009 |
url |
http://gala.gre.ac.uk/id/eprint/2060/ https://doi.org/10.1007/s10886-009-9641-5 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Molnár, Béla, Kárpáti, Zsolt, Szőcs, Gábor and Hall, David R. orcid:0000-0002-7887-466X (2009) Identification of female-produced sex pheromone of the honey locust gall midge, Dasineura gleditchiae. Journal of Chemical Ecology, 35 (6). pp. 706-714. ISSN 0098-0331 (Print), 1573-1561 (Online) (doi:https://doi.org/10.1007/s10886-009-9641-5 <https://doi.org/10.1007/s10886-009-9641-5>) |
op_doi |
https://doi.org/10.1007/s10886-009-9641-5 |
container_title |
Journal of Chemical Ecology |
container_volume |
35 |
container_issue |
6 |
container_start_page |
706 |
op_container_end_page |
714 |
_version_ |
1766261455157460992 |