Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offsp...
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ftunivgreenwich:oai:gala.gre.ac.uk:20394 2023-05-15T13:28:13+02:00 Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna Junack Tomkiewicz, Jonna Butts, Ian A.E. 2018-06-13 application/pdf http://gala.gre.ac.uk/id/eprint/20394/ http://gala.gre.ac.uk/id/eprint/20394/9/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_%28OA%29_2018.pdf http://gala.gre.ac.uk/id/eprint/20394/3/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_2018.pdf https://doi.org/10.1371/journal.pone.0198294 en eng Public Library of Science (PLoS) http://gala.gre.ac.uk/id/eprint/20394/9/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_%28OA%29_2018.pdf http://gala.gre.ac.uk/id/eprint/20394/3/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_2018.pdf Politis, Sebastian N., Mazurais, David, Servili, Arianna, Zambonino-Infante, Jose-Luis, Miest, Joanna Junack orcid:0000-0003-2534-0530 , Tomkiewicz, Jonna and Butts, Ian A.E. (2018) Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level. PLoS ONE, 13 (6):e0198294. ISSN 1932-6203 (Online) (doi:https://doi.org/10.1371/journal.pone.0198294 <https://doi.org/10.1371/journal.pone.0198294>) cc_by_4 GE Environmental Sciences Article PeerReviewed 2018 ftunivgreenwich https://doi.org/10.1371/journal.pone.0198294 2023-03-26T20:28:51Z European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2α, nkcc2β, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thrαA, thrαB, thrβB, dio1, dio2, dio3) were differentially expressed throughout larval development, while nkcc1α, nkcc2β, aqp3, aqp1dup, aqpe, hsp90, thrαA and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated osmoregulation processes at such early life stage of this species. Article in Journal/Newspaper Anguilla anguilla European eel University of Greenwich: Greenwich Academic Literature Archive PLOS ONE 13 6 e0198294 |
institution |
Open Polar |
collection |
University of Greenwich: Greenwich Academic Literature Archive |
op_collection_id |
ftunivgreenwich |
language |
English |
topic |
GE Environmental Sciences |
spellingShingle |
GE Environmental Sciences Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna Junack Tomkiewicz, Jonna Butts, Ian A.E. Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
topic_facet |
GE Environmental Sciences |
description |
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2α, nkcc2β, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thrαA, thrαB, thrβB, dio1, dio2, dio3) were differentially expressed throughout larval development, while nkcc1α, nkcc2β, aqp3, aqp1dup, aqpe, hsp90, thrαA and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated osmoregulation processes at such early life stage of this species. |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna Junack Tomkiewicz, Jonna Butts, Ian A.E. |
author_facet |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna Junack Tomkiewicz, Jonna Butts, Ian A.E. |
author_sort |
Politis, Sebastian N. |
title |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_short |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_fullStr |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full_unstemmed |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_sort |
salinity reduction benefits european eel larvae: insights at the morphological and molecular level |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
http://gala.gre.ac.uk/id/eprint/20394/ http://gala.gre.ac.uk/id/eprint/20394/9/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_%28OA%29_2018.pdf http://gala.gre.ac.uk/id/eprint/20394/3/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_2018.pdf https://doi.org/10.1371/journal.pone.0198294 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
http://gala.gre.ac.uk/id/eprint/20394/9/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_%28OA%29_2018.pdf http://gala.gre.ac.uk/id/eprint/20394/3/20394%20MIEST_Salinity_Reduction_Benefits_European_Eel_Larvae_2018.pdf Politis, Sebastian N., Mazurais, David, Servili, Arianna, Zambonino-Infante, Jose-Luis, Miest, Joanna Junack orcid:0000-0003-2534-0530 , Tomkiewicz, Jonna and Butts, Ian A.E. (2018) Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level. PLoS ONE, 13 (6):e0198294. ISSN 1932-6203 (Online) (doi:https://doi.org/10.1371/journal.pone.0198294 <https://doi.org/10.1371/journal.pone.0198294>) |
op_rights |
cc_by_4 |
op_doi |
https://doi.org/10.1371/journal.pone.0198294 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e0198294 |
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1766402902143795200 |