Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae
Temperature is important for optimization of rearing conditions in aquaculture, especially during the “critical” early life history stages of fish. Here, we experimentally investigated the thermal tolerance range for early larval European eel, Anguilla anguilla. We assessed the impact of temperature...
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Online Access: | http://gala.gre.ac.uk/id/eprint/17309/ http://gala.gre.ac.uk/id/eprint/17309/8/17309%20MIEST_Morphological_Development_of_European_Eel_%20%28OA%29_2017.pdf https://doi.org/10.1371/journal.pone.0182726 |
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ftunivgreenwich:oai:gala.gre.ac.uk:17309 2023-05-15T13:27:32+02:00 Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. 2017-08-14 application/pdf http://gala.gre.ac.uk/id/eprint/17309/ http://gala.gre.ac.uk/id/eprint/17309/8/17309%20MIEST_Morphological_Development_of_European_Eel_%20%28OA%29_2017.pdf https://doi.org/10.1371/journal.pone.0182726 en eng Public Library of Science (PLoS) http://gala.gre.ac.uk/id/eprint/17309/8/17309%20MIEST_Morphological_Development_of_European_Eel_%20%28OA%29_2017.pdf Politis, Sebastian N., Mazurais, David, Servili, Arianna, Zambonino-Infante, Jose-Luis, Miest, Joanna J. orcid:0000-0003-2534-0530 , Sørensen, Sune R., Tomkiewicz, Jonna and Butts, Ian A. E. (2017) Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae. PLoS ONE. ISSN 1932-6203 (Online) (doi:https://doi.org/10.1371/journal.pone.0182726 <https://doi.org/10.1371/journal.pone.0182726>) cc_by_4 SH Aquaculture. Fisheries. Angling Article PeerReviewed 2017 ftunivgreenwich https://doi.org/10.1371/journal.pone.0182726 2023-03-26T20:28:02Z Temperature is important for optimization of rearing conditions in aquaculture, especially during the “critical” early life history stages of fish. Here, we experimentally investigated the thermal tolerance range for early larval European eel, Anguilla anguilla. We assessed the impact of temperature (16, 18, 20, 22, and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance. Statistical models were applied to investigate temperature effects on larval morphology and gene expression throughout early larval development (0 to 18 days post-hatch) and at specific developmental stages (Stages 1-3). Stage 1 represents the day of hatch, Stage 2 represents the timing of jaw/teeth formation, and Stage 3 represents the first-feeding stage. Results showed that hatch success, yolk utilization efficiency, and survival decreased, while deformities, yolk utilization, and growth rates increased with increasing temperature (16-22°C). Increasing temperature accelerated larval development and in real-time, gene expression patterns were delayed when larvae were reared at cold temperatures (16°C) or accelerated when reared at warm temperatures (20-22°C). In developmental time, the expression of all targeted genes were affected by larval stage and expression of hsp90 and gh was furthermore affected by temperature. We conclude that 24°C is deleterious, while 16°C and 22°C (90% deformities) represent the lower and upper thermal tolerance limits. The combination of highest larval survival, lowest incidence of larval deformities at hatch, high yolk utilization efficiency, high gh and low hsp expression, suggest 18°C as the optimal temperature in larval culture of European eel. Furthermore, our results suggest that the still enigmatic early life history stages of European eel may inhabit the deeper layer of the Sargasso Sea and indicate ... Article in Journal/Newspaper Anguilla anguilla University of Greenwich: Greenwich Academic Literature Archive PLOS ONE 12 8 e0182726 |
institution |
Open Polar |
collection |
University of Greenwich: Greenwich Academic Literature Archive |
op_collection_id |
ftunivgreenwich |
language |
English |
topic |
SH Aquaculture. Fisheries. Angling |
spellingShingle |
SH Aquaculture. Fisheries. Angling Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
topic_facet |
SH Aquaculture. Fisheries. Angling |
description |
Temperature is important for optimization of rearing conditions in aquaculture, especially during the “critical” early life history stages of fish. Here, we experimentally investigated the thermal tolerance range for early larval European eel, Anguilla anguilla. We assessed the impact of temperature (16, 18, 20, 22, and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance. Statistical models were applied to investigate temperature effects on larval morphology and gene expression throughout early larval development (0 to 18 days post-hatch) and at specific developmental stages (Stages 1-3). Stage 1 represents the day of hatch, Stage 2 represents the timing of jaw/teeth formation, and Stage 3 represents the first-feeding stage. Results showed that hatch success, yolk utilization efficiency, and survival decreased, while deformities, yolk utilization, and growth rates increased with increasing temperature (16-22°C). Increasing temperature accelerated larval development and in real-time, gene expression patterns were delayed when larvae were reared at cold temperatures (16°C) or accelerated when reared at warm temperatures (20-22°C). In developmental time, the expression of all targeted genes were affected by larval stage and expression of hsp90 and gh was furthermore affected by temperature. We conclude that 24°C is deleterious, while 16°C and 22°C (90% deformities) represent the lower and upper thermal tolerance limits. The combination of highest larval survival, lowest incidence of larval deformities at hatch, high yolk utilization efficiency, high gh and low hsp expression, suggest 18°C as the optimal temperature in larval culture of European eel. Furthermore, our results suggest that the still enigmatic early life history stages of European eel may inhabit the deeper layer of the Sargasso Sea and indicate ... |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. |
author_facet |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. |
author_sort |
Politis, Sebastian N. |
title |
Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
title_short |
Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
title_full |
Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
title_fullStr |
Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
title_full_unstemmed |
Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae |
title_sort |
temperature effects on gene expression and morphological development of european eel, anguilla anguilla larvae |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
http://gala.gre.ac.uk/id/eprint/17309/ http://gala.gre.ac.uk/id/eprint/17309/8/17309%20MIEST_Morphological_Development_of_European_Eel_%20%28OA%29_2017.pdf https://doi.org/10.1371/journal.pone.0182726 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://gala.gre.ac.uk/id/eprint/17309/8/17309%20MIEST_Morphological_Development_of_European_Eel_%20%28OA%29_2017.pdf Politis, Sebastian N., Mazurais, David, Servili, Arianna, Zambonino-Infante, Jose-Luis, Miest, Joanna J. orcid:0000-0003-2534-0530 , Sørensen, Sune R., Tomkiewicz, Jonna and Butts, Ian A. E. (2017) Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae. PLoS ONE. ISSN 1932-6203 (Online) (doi:https://doi.org/10.1371/journal.pone.0182726 <https://doi.org/10.1371/journal.pone.0182726>) |
op_rights |
cc_by_4 |
op_doi |
https://doi.org/10.1371/journal.pone.0182726 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
8 |
container_start_page |
e0182726 |
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1766398988661030912 |