Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials.

• Published in: Catalysts
• Main Authors: Luna, Diego, Verdugo-Escamilla, Cristóbal
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI 2021
• Subjects: Biofuel; Ecodiesel; Biodiésel; Commercial CALB lipase; Ordered mesoporous materials (PMO); Amorphous siliceous material MS3030; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10481/71775; https://doi.org/10.3390/catal11111350

This research is supported by MINECO-ENE2016-81013-R (AEI/FEDER, EU), MICIIN (Project ref. PID2019-104953RB-100), Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía (UCO-FEDER Project CATOLIVAL, ref. 1264113-R, 2018, and Project ref. P18-RT-4822) and FEDER Funds for financial support. The Government of Ireland Postdoctoral Fellowship Programme-2015 (GOIPD/2015/287) is thankfully acknowledge by V.G.-P. This research article is part of the doctoral thesis of C.L., directed by professors D.L., F.M.B. and C.V.-E., who in a general way conceived and designed the experiments and wrote the paper. V.G.-P. collaborated in a decisive way contributing everything concerning the investigated inorganic supports. R.E., F.J.L.-T., J.C. and L.A.-D. have made also substantive intellectual contributions to this study, substantial input to conception and design, as well as to the acquisition, analysis and interpretation of data. A.A.R. and R.E. were also involved in drafting and revising the manuscript, so that everyone gave final approval to the current version to be published in Catalysts journal. All authors have read and agreed to the published version of the manuscript. The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the bio-catalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candi-dates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel. Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía MICIIN PID2019-104953RB-100 MINECO-ENE2016-81013-R UCO-FEDER 1264113-R, GOIPD/2015/287, P18-RT-4822 European Commission European Regional Development Fund Agencia Estatal de Investigación