| Summary: | One major challenge in aquaculture is the issue of fishmeal replacement as a protein source in aquafeeds. It is agreed that the rate of demand has now outpaced the rate of supply due to the rapid expansion of aquaculture. In Sweden, work is being done to establish a knowledge base for the development of sustainable marine aquaculture, focusing on two species: Atlantic wolffish, Anarhichas lupus and European lobster, Hommarus gammarus, as well as on two novel protein ingredients. The goal of this thesis was to contribute to a knowledge base for the farming biology and culture operations of the two species, with a circular economy model and minimal environmental impact in mind in line with UNs agenda 2030. In paper I, work was done to optimize the pH-shift process, a novel protein extraction technology with potential to produce a highly concentrated protein ingredient from industrial seafood by-products. Three combinations of herring by-products were chosen along with two different process settings and differences in final proximate composition were characterized. Results showed the alkaline version of the process gave significantly higher protein yields and all forms of by-products were deemed as promising. Paper II initiated four feeding experiments (novel feed types, feeding regime and feed size and cannibalism effects) on growth and survival, to inform and update husbandry protocols in H. gammarus. Overall, we found that feed offered six times daily, small‐grade dry feed (250–360 μm) and larvae fed different proportions of dry feed and/or conspecifics in both communal and individual rearing systems all improved growth and survival rates. This underlines the impact of cannibalism on survival in H. gammarus larviculture. In paper III we examined the suitability of locally produced, novel protein sources from by-products on the growth of recently metamorphosed post-larva. We found that, that a diet containing a proportion of shrimp, created from local industry by-products, was the best source of a sustainable ...
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