Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier
The effect of environmental factors and pathogens on the intestinal epithelium of fish has received increased attention in recent years. Studies focusing on effects of stress, nutrient uptake as well as vegetable ingredients in fish feed have all shown that the intestine is affected by environmental...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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2013
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Online Access: | http://hdl.handle.net/2077/32780 |
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ftunivgoeteborg:oai:gupea.ub.gu.se:2077/32780 |
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openpolar |
institution |
Open Polar |
collection |
University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
op_collection_id |
ftunivgoeteborg |
language |
English |
topic |
Inflammation IPNV Poly I:C Recombinant cytokines CD8 MHC-I Claudins Permeability Environmental stress Cortisol DNA constructs IL IFN |
spellingShingle |
Inflammation IPNV Poly I:C Recombinant cytokines CD8 MHC-I Claudins Permeability Environmental stress Cortisol DNA constructs IL IFN Niklasson, Lars Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
topic_facet |
Inflammation IPNV Poly I:C Recombinant cytokines CD8 MHC-I Claudins Permeability Environmental stress Cortisol DNA constructs IL IFN |
description |
The effect of environmental factors and pathogens on the intestinal epithelium of fish has received increased attention in recent years. Studies focusing on effects of stress, nutrient uptake as well as vegetable ingredients in fish feed have all shown that the intestine is affected by environmental factors. The signs of inflammation during exposure to detrimental environmental conditions have brought to attention the local immune system in the gut. The gut is further one of the main routes for pathogen infection in fish. Therefore this thesis aims at investigating the mucosal immune factors and systems that are affected by environmental stressors and pathogen interactions. In this thesis the effect of long term environmental stress on the mucosal intestinal epithelium was investigated. Results showed an ongoing inflammation in the intestine that was manifested as a compromised barrier integrity, infiltration of immune cells and an affected immune response. Atlantic salmon was co-habitant infected with infectious pancreatic necrosis virus as well as immune challenged with the viral mimicker, double stranded RNA Poly I:C, where after the mucosal immune response was studied. Both treatments clearly demonstrated an antiviral response including alterations of IFN type I and the Mx protein. When the fish were exposed to a stressor and immune stimulation in combination, the fish immune response was delayed. This stresses the importance of minimize stressful situations for the animals in, for example aquaculture. The demonstrated increase in intestinal epithelial permeability together with inductions of the mucosal immune system raises the question of whether stress or inflammation is the causative agent of the barrier dysfunction. To address this, the effect of the immune system on the intestinal epithelia was assessed using an in vitro Ussing chamber approach in which the intestinal epithelia was exposed to recombinant cytokines. Exposure to IL-1β and IL-6 showed negative impact on the intestinal permeability, ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Niklasson, Lars |
author_facet |
Niklasson, Lars |
author_sort |
Niklasson, Lars |
title |
Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
title_short |
Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
title_full |
Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
title_fullStr |
Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
title_full_unstemmed |
Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier |
title_sort |
intestinal mucosal immunology of salmonids response to stress and infection and crosstalk with the physical barrier |
publishDate |
2013 |
url |
http://hdl.handle.net/2077/32780 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
I. Lars Niklasson; Henrik Sundh; F Fridell; GL Taranger; Kristina Sundell. Disturbance of the intestinal mucosal immune system of farmed Atlantic salmon (Salmo salar), in response to long-term hypoxic conditions. 2011 31:1050-4648 ::doi::10.1016/j.fsi.2011.09.011 II. Sundh H.; Niklasson L.; Finne-Fridell F.; Ellis T.; Taranger G L., Pettersen E F.; Wergeland H I.; Sundell K. High stocking density and poor water quality disturbs the intestinal physical and immunological barriers of the Atlantic salmon. (Revised manuscript for publication in Fish and Shellfish Immunology) III. Bjørn Olav Kvamme; Koestan Gadan; Frode Finne-Fridell; Lars Niklasson; Henrik Sundh; Kristina Sundell; Geir Lasse Taranger; Oystein Evensen. Modulation of innate immune responses in Atlantic salmon by chronic hypoxia-induced stress. 2013 31:1095-9947::doi::10.1016/j.fsi.2012.10.006 IV. Niklasson L.; Sundh H.; Olsen R-E.; Jutfelt F.; Skjødt K.; Nilsen T O.; Sundell K. Cortisol effects on the intestinal mucosal immune responses during cohabitant challenge with IPNV in Atlantic salmon (Salmo Salar). (Submitted for publication in PLOS ONE) V. Niklasson L.; Sundell K.; Martin S.; Secombes C.; Sundh H. Recombinant cytokines interleukin 1 beta and interleukin 6 increases intestinal epithelial permeability in Rainbow trout (Oncorhynchus mykiss). (Manuscript) 978-91-628-8717-9 http://hdl.handle.net/2077/32780 |
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1781058109955899392 |
spelling |
ftunivgoeteborg:oai:gupea.ub.gu.se:2077/32780 2023-10-29T02:35:07+01:00 Intestinal Mucosal Immunology of Salmonids Response to Stress and Infection and Crosstalk with the Physical Barrier Intestinal Mucosal Immunology of Salmonids Niklasson, Lars 2013-05-15 application/pdf http://hdl.handle.net/2077/32780 eng eng I. Lars Niklasson; Henrik Sundh; F Fridell; GL Taranger; Kristina Sundell. Disturbance of the intestinal mucosal immune system of farmed Atlantic salmon (Salmo salar), in response to long-term hypoxic conditions. 2011 31:1050-4648 ::doi::10.1016/j.fsi.2011.09.011 II. Sundh H.; Niklasson L.; Finne-Fridell F.; Ellis T.; Taranger G L., Pettersen E F.; Wergeland H I.; Sundell K. High stocking density and poor water quality disturbs the intestinal physical and immunological barriers of the Atlantic salmon. (Revised manuscript for publication in Fish and Shellfish Immunology) III. Bjørn Olav Kvamme; Koestan Gadan; Frode Finne-Fridell; Lars Niklasson; Henrik Sundh; Kristina Sundell; Geir Lasse Taranger; Oystein Evensen. Modulation of innate immune responses in Atlantic salmon by chronic hypoxia-induced stress. 2013 31:1095-9947::doi::10.1016/j.fsi.2012.10.006 IV. Niklasson L.; Sundh H.; Olsen R-E.; Jutfelt F.; Skjødt K.; Nilsen T O.; Sundell K. Cortisol effects on the intestinal mucosal immune responses during cohabitant challenge with IPNV in Atlantic salmon (Salmo Salar). (Submitted for publication in PLOS ONE) V. Niklasson L.; Sundell K.; Martin S.; Secombes C.; Sundh H. Recombinant cytokines interleukin 1 beta and interleukin 6 increases intestinal epithelial permeability in Rainbow trout (Oncorhynchus mykiss). (Manuscript) 978-91-628-8717-9 http://hdl.handle.net/2077/32780 Inflammation IPNV Poly I:C Recombinant cytokines CD8 MHC-I Claudins Permeability Environmental stress Cortisol DNA constructs IL IFN Text Doctoral thesis Doctor of Philosophy 2013 ftunivgoeteborg 2023-10-04T21:13:10Z The effect of environmental factors and pathogens on the intestinal epithelium of fish has received increased attention in recent years. Studies focusing on effects of stress, nutrient uptake as well as vegetable ingredients in fish feed have all shown that the intestine is affected by environmental factors. The signs of inflammation during exposure to detrimental environmental conditions have brought to attention the local immune system in the gut. The gut is further one of the main routes for pathogen infection in fish. Therefore this thesis aims at investigating the mucosal immune factors and systems that are affected by environmental stressors and pathogen interactions. In this thesis the effect of long term environmental stress on the mucosal intestinal epithelium was investigated. Results showed an ongoing inflammation in the intestine that was manifested as a compromised barrier integrity, infiltration of immune cells and an affected immune response. Atlantic salmon was co-habitant infected with infectious pancreatic necrosis virus as well as immune challenged with the viral mimicker, double stranded RNA Poly I:C, where after the mucosal immune response was studied. Both treatments clearly demonstrated an antiviral response including alterations of IFN type I and the Mx protein. When the fish were exposed to a stressor and immune stimulation in combination, the fish immune response was delayed. This stresses the importance of minimize stressful situations for the animals in, for example aquaculture. The demonstrated increase in intestinal epithelial permeability together with inductions of the mucosal immune system raises the question of whether stress or inflammation is the causative agent of the barrier dysfunction. To address this, the effect of the immune system on the intestinal epithelia was assessed using an in vitro Ussing chamber approach in which the intestinal epithelia was exposed to recombinant cytokines. Exposure to IL-1β and IL-6 showed negative impact on the intestinal permeability, ... Doctoral or Postdoctoral Thesis Atlantic salmon University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |