Environmental, nutritional and endocrine regulation of metabolic processes in fish
Due to seasonal variations in temperature and food availability, fish in temperate regions should be able to make metabolic adjustments to ensure that enough energy is available for the maintenance of basal processes. The major aim of this thesis was to elucidate how the physiology and lipid metabol...
Main Author: | |
---|---|
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
Published: |
2011
|
Subjects: | |
Online Access: | http://hdl.handle.net/2077/27888 |
id |
ftunivgoeteborg:oai:gupea.ub.gu.se:2077/27888 |
---|---|
record_format |
openpolar |
spelling |
ftunivgoeteborg:oai:gupea.ub.gu.se:2077/27888 2023-10-29T02:39:50+01:00 Environmental, nutritional and endocrine regulation of metabolic processes in fish Kullgren, Andreas 2011-11-18 application/pdf http://hdl.handle.net/2077/27888 eng eng Kullgren A, Samuelsson LM, Larsson DGJ, Björnsson BTh, Jönsson Bergman E, 2010. A metabolomics approach to elucidate effects of food deprivation in juvenile rainbow trout (Oncorhynchus mykiss). American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 299 (6):R1440-R1448.::doi::10.1152/ajpregu.00281.2010 Kullgren A, Jutfelt F, Fontanillas R, Sundell K, Samuelsson LM, Wiklander K, Kling P, Koppe W, Larsson DGJ, Björnsson BTh, Jönsson E. The impact of temperature on the metabolome and endocrine metabolic signaling in Atlantic salmon (Salmo salar). Manuscript Kullgren A, Björnsson BTh, Jönsson E. Growth hormone regulates lipid metabolism-related genes in rainbow trout in vivo. Manuscript Kullgren A, Jönsson E. Lipid uptake and mobilization in isolated rainbow trout hepatocytes and adipocytes are affected by fatty acids and temperature. Manuscript 978-91-628-8390-4 http://hdl.handle.net/2077/27888 lipids metabolism temperature fasting growth hormone ghrelin NMR metabolomics Salmo salar Oncorhynchus mykiss Text Doctoral thesis Doctor of Philosophy 2011 ftunivgoeteborg 2023-10-04T21:14:15Z Due to seasonal variations in temperature and food availability, fish in temperate regions should be able to make metabolic adjustments to ensure that enough energy is available for the maintenance of basal processes. The major aim of this thesis was to elucidate how the physiology and lipid metabolism of salmonid fish is affected by temperature and food availability, and to clarify aspects of the endocrine control of lipid metabolism. In this thesis, the effects of increased temperature or reduced food availability were studied in salmonids by employing a non-prejudiced metabolomics approach to assess the physiological responses. Detailed information on the abundance of specific amino acids, lipid classes, fatty acids and other metabolites in tissue extracts and plasma was obtained by nuclear magnetic resonance (NMR) based metabolomics. NMR-based metabolomics were successfully employed and proved to be applicable to studying metabolic fluxes in fish, providing data on novel and integrated responses. The results show similar changes in lipid metabolism during food deprivation and elevated temperature. The observed responses included increased plasma very-low-density lipoprotein (VLDL) and unsaturated fatty acids (FAs) concurrent with decreased high-density lipoprotein (HDL) and choline. The changes during starvation also involved changes in amino acids and glycogen that indicate that amino acids are used for gluconeogenesis in the liver to preserve glycogen stores. Growth hormone (GH) has both lipolytic and lipogenic effects. To further elucidate the mechanisms of GH action on salmonid lipid metabolism, the effects of GH in vivo on the transcription of several key lipid metabolism enzymes in various tissues were investigated. GH inhibited the hepatic expression of lipoprotein lipase (LPL) thereby decreasing hepatic lipid uptake. Hormone-sensitive lipase (HSL) mRNA expression was not increased by GH in any of the studied tissues, suggesting that the well-known GHinduced lipolysis is regulated on posttranslational ... Doctoral or Postdoctoral Thesis Salmo salar University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
institution |
Open Polar |
collection |
University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
op_collection_id |
ftunivgoeteborg |
language |
English |
topic |
lipids metabolism temperature fasting growth hormone ghrelin NMR metabolomics Salmo salar Oncorhynchus mykiss |
spellingShingle |
lipids metabolism temperature fasting growth hormone ghrelin NMR metabolomics Salmo salar Oncorhynchus mykiss Kullgren, Andreas Environmental, nutritional and endocrine regulation of metabolic processes in fish |
topic_facet |
lipids metabolism temperature fasting growth hormone ghrelin NMR metabolomics Salmo salar Oncorhynchus mykiss |
description |
Due to seasonal variations in temperature and food availability, fish in temperate regions should be able to make metabolic adjustments to ensure that enough energy is available for the maintenance of basal processes. The major aim of this thesis was to elucidate how the physiology and lipid metabolism of salmonid fish is affected by temperature and food availability, and to clarify aspects of the endocrine control of lipid metabolism. In this thesis, the effects of increased temperature or reduced food availability were studied in salmonids by employing a non-prejudiced metabolomics approach to assess the physiological responses. Detailed information on the abundance of specific amino acids, lipid classes, fatty acids and other metabolites in tissue extracts and plasma was obtained by nuclear magnetic resonance (NMR) based metabolomics. NMR-based metabolomics were successfully employed and proved to be applicable to studying metabolic fluxes in fish, providing data on novel and integrated responses. The results show similar changes in lipid metabolism during food deprivation and elevated temperature. The observed responses included increased plasma very-low-density lipoprotein (VLDL) and unsaturated fatty acids (FAs) concurrent with decreased high-density lipoprotein (HDL) and choline. The changes during starvation also involved changes in amino acids and glycogen that indicate that amino acids are used for gluconeogenesis in the liver to preserve glycogen stores. Growth hormone (GH) has both lipolytic and lipogenic effects. To further elucidate the mechanisms of GH action on salmonid lipid metabolism, the effects of GH in vivo on the transcription of several key lipid metabolism enzymes in various tissues were investigated. GH inhibited the hepatic expression of lipoprotein lipase (LPL) thereby decreasing hepatic lipid uptake. Hormone-sensitive lipase (HSL) mRNA expression was not increased by GH in any of the studied tissues, suggesting that the well-known GHinduced lipolysis is regulated on posttranslational ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Kullgren, Andreas |
author_facet |
Kullgren, Andreas |
author_sort |
Kullgren, Andreas |
title |
Environmental, nutritional and endocrine regulation of metabolic processes in fish |
title_short |
Environmental, nutritional and endocrine regulation of metabolic processes in fish |
title_full |
Environmental, nutritional and endocrine regulation of metabolic processes in fish |
title_fullStr |
Environmental, nutritional and endocrine regulation of metabolic processes in fish |
title_full_unstemmed |
Environmental, nutritional and endocrine regulation of metabolic processes in fish |
title_sort |
environmental, nutritional and endocrine regulation of metabolic processes in fish |
publishDate |
2011 |
url |
http://hdl.handle.net/2077/27888 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
Kullgren A, Samuelsson LM, Larsson DGJ, Björnsson BTh, Jönsson Bergman E, 2010. A metabolomics approach to elucidate effects of food deprivation in juvenile rainbow trout (Oncorhynchus mykiss). American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 299 (6):R1440-R1448.::doi::10.1152/ajpregu.00281.2010 Kullgren A, Jutfelt F, Fontanillas R, Sundell K, Samuelsson LM, Wiklander K, Kling P, Koppe W, Larsson DGJ, Björnsson BTh, Jönsson E. The impact of temperature on the metabolome and endocrine metabolic signaling in Atlantic salmon (Salmo salar). Manuscript Kullgren A, Björnsson BTh, Jönsson E. Growth hormone regulates lipid metabolism-related genes in rainbow trout in vivo. Manuscript Kullgren A, Jönsson E. Lipid uptake and mobilization in isolated rainbow trout hepatocytes and adipocytes are affected by fatty acids and temperature. Manuscript 978-91-628-8390-4 http://hdl.handle.net/2077/27888 |
_version_ |
1781067279124922368 |