| Summary: | Due to seasonal variations in temperature and food availability, fish in temperate regions should be able to make metabolic adjustments to ensure that enough energy is available for the maintenance of basal processes. The major aim of this thesis was to elucidate how the physiology and lipid metabolism of salmonid fish is affected by temperature and food availability, and to clarify aspects of the endocrine control of lipid metabolism. In this thesis, the effects of increased temperature or reduced food availability were studied in salmonids by employing a non-prejudiced metabolomics approach to assess the physiological responses. Detailed information on the abundance of specific amino acids, lipid classes, fatty acids and other metabolites in tissue extracts and plasma was obtained by nuclear magnetic resonance (NMR) based metabolomics. NMR-based metabolomics were successfully employed and proved to be applicable to studying metabolic fluxes in fish, providing data on novel and integrated responses. The results show similar changes in lipid metabolism during food deprivation and elevated temperature. The observed responses included increased plasma very-low-density lipoprotein (VLDL) and unsaturated fatty acids (FAs) concurrent with decreased high-density lipoprotein (HDL) and choline. The changes during starvation also involved changes in amino acids and glycogen that indicate that amino acids are used for gluconeogenesis in the liver to preserve glycogen stores. Growth hormone (GH) has both lipolytic and lipogenic effects. To further elucidate the mechanisms of GH action on salmonid lipid metabolism, the effects of GH in vivo on the transcription of several key lipid metabolism enzymes in various tissues were investigated. GH inhibited the hepatic expression of lipoprotein lipase (LPL) thereby decreasing hepatic lipid uptake. Hormone-sensitive lipase (HSL) mRNA expression was not increased by GH in any of the studied tissues, suggesting that the well-known GHinduced lipolysis is regulated on posttranslational ...
|
|---|