Studies of fish responses to the antifoulant medetomidine
ABSTRACT Growth of marine organisms, fouling, on man-made constructions submerged in the water is regarded as a major problem. For vessels, fouling increases drag and thereby fuel consumption, wherefore antifouling paints are used. Traditionally, they contain toxic compounds, and several of these ha...
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ftunivgoeteborg:oai:gupea.ub.gu.se:2077/22081 2023-10-29T02:34:57+01:00 Studies of fish responses to the antifoulant medetomidine Lennquist, Anna 2010-03-31T11:02:12Z application/pdf http://hdl.handle.net/2077/22081 eng eng I Lennquist A, Hilvarsson A, Förlin L. 2010. Responses in fish exposed to medetomidine, a new antifouling agent. Marine Environmental Research. In press.::doi::10.1016/j.marenvres.2010.01.004 II Lennquist A, Lindblad Mårtensson LGE, Björnsson BTh, Förlin L. 2010. The effects of medetomidine, a new antifouling agent, on rainbow trout physiology. Manuscript. III Lennquist A, Celander MC, Förlin L. 2008. Effects of mdetomidine on hepatic EROD activity in three species of fish. Ecotoxicology and Environmental safety 69.::doi::10.1016/j.ecoenv.2007.05.004 IV Lennquist A, Hedberg D, Lindblad Mårtensson LGE, Kristiansson E, Förlin L, 2009. Colour and melanophore function in rainbow trout after long term exposure to the new antifoulant medetomidine. Under revision. V Lennquist A, Asker N, Kristiansson E, Brenthel A, , Björnsson BTh, Kling P, Larsson DGJ, and Förlin L. 2010. Physiology and gene expression in rainbow trout (Oncorhynchus mykiss) after long term exposure to the new antifoulant medetomidine. Manuscript. 978-91-628-8082-8 http://hdl.handle.net/2077/22081 antifouling fish medetomidine ecotoxicology biomarker Text Doctoral Theses Doctor of Philosophy 2010 ftunivgoeteborg 2023-10-04T21:19:18Z ABSTRACT Growth of marine organisms, fouling, on man-made constructions submerged in the water is regarded as a major problem. For vessels, fouling increases drag and thereby fuel consumption, wherefore antifouling paints are used. Traditionally, they contain toxic compounds, and several of these have unwanted effects in the environment. Today the search for environmentally acceptable and efficient alternatives is intense. Medetomidine, originally used as a veterinary sedative, inhibits barnacle settling at nanomolar concentrations. It is presently under evaluation for use as an antifouling agent. The studies within this thesis were performed to investigate medetomidine responses in fish. The focus was to identify early effects, occurring from low concentrations. Studies have been performed in the species rainbow trout, Atlantic cod, turbot, Atlantic salmon and three spined stickleback. Exposure time vary from 1 up to 54 days, and a set of parameters have been investigated including biochemical biomarkers, growth and related parameters, behaviour and large scale gene expression. Paleness is the most obvious effect of medetomidine in fish and appears from 0.5 to 50 nM, depending on species. Colour was observed and quantified, and the function of melanophores (pigment cells) after long term exposure to medetomidine was investigated. It is suggested that melanophores are functional after treatment, and thus the colour change may be reversible. Although not lethal per-see, paleness may have consequences for fish predator-prey interactions (camouflage), social signalling and UV protection. Medetomidine also showed to affect the activity of the hepatic enzyme Cytochrome P4501A (CYP1A), measured as EROD activity. A minor increase in activity was observed in vivo in several of the investigated species. In vitro, medetomidine showed instead to be a potent inhibitor of EROD activity with median inhibition values (IC50) in the nanomolar range. An inhibited CYP1A activity may interfere with fish detoxification of toxicants ... Doctoral or Postdoctoral Thesis atlantic cod Atlantic salmon Turbot University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
institution |
Open Polar |
collection |
University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
op_collection_id |
ftunivgoeteborg |
language |
English |
topic |
antifouling fish medetomidine ecotoxicology biomarker |
spellingShingle |
antifouling fish medetomidine ecotoxicology biomarker Lennquist, Anna Studies of fish responses to the antifoulant medetomidine |
topic_facet |
antifouling fish medetomidine ecotoxicology biomarker |
description |
ABSTRACT Growth of marine organisms, fouling, on man-made constructions submerged in the water is regarded as a major problem. For vessels, fouling increases drag and thereby fuel consumption, wherefore antifouling paints are used. Traditionally, they contain toxic compounds, and several of these have unwanted effects in the environment. Today the search for environmentally acceptable and efficient alternatives is intense. Medetomidine, originally used as a veterinary sedative, inhibits barnacle settling at nanomolar concentrations. It is presently under evaluation for use as an antifouling agent. The studies within this thesis were performed to investigate medetomidine responses in fish. The focus was to identify early effects, occurring from low concentrations. Studies have been performed in the species rainbow trout, Atlantic cod, turbot, Atlantic salmon and three spined stickleback. Exposure time vary from 1 up to 54 days, and a set of parameters have been investigated including biochemical biomarkers, growth and related parameters, behaviour and large scale gene expression. Paleness is the most obvious effect of medetomidine in fish and appears from 0.5 to 50 nM, depending on species. Colour was observed and quantified, and the function of melanophores (pigment cells) after long term exposure to medetomidine was investigated. It is suggested that melanophores are functional after treatment, and thus the colour change may be reversible. Although not lethal per-see, paleness may have consequences for fish predator-prey interactions (camouflage), social signalling and UV protection. Medetomidine also showed to affect the activity of the hepatic enzyme Cytochrome P4501A (CYP1A), measured as EROD activity. A minor increase in activity was observed in vivo in several of the investigated species. In vitro, medetomidine showed instead to be a potent inhibitor of EROD activity with median inhibition values (IC50) in the nanomolar range. An inhibited CYP1A activity may interfere with fish detoxification of toxicants ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Lennquist, Anna |
author_facet |
Lennquist, Anna |
author_sort |
Lennquist, Anna |
title |
Studies of fish responses to the antifoulant medetomidine |
title_short |
Studies of fish responses to the antifoulant medetomidine |
title_full |
Studies of fish responses to the antifoulant medetomidine |
title_fullStr |
Studies of fish responses to the antifoulant medetomidine |
title_full_unstemmed |
Studies of fish responses to the antifoulant medetomidine |
title_sort |
studies of fish responses to the antifoulant medetomidine |
publishDate |
2010 |
url |
http://hdl.handle.net/2077/22081 |
genre |
atlantic cod Atlantic salmon Turbot |
genre_facet |
atlantic cod Atlantic salmon Turbot |
op_relation |
I Lennquist A, Hilvarsson A, Förlin L. 2010. Responses in fish exposed to medetomidine, a new antifouling agent. Marine Environmental Research. In press.::doi::10.1016/j.marenvres.2010.01.004 II Lennquist A, Lindblad Mårtensson LGE, Björnsson BTh, Förlin L. 2010. The effects of medetomidine, a new antifouling agent, on rainbow trout physiology. Manuscript. III Lennquist A, Celander MC, Förlin L. 2008. Effects of mdetomidine on hepatic EROD activity in three species of fish. Ecotoxicology and Environmental safety 69.::doi::10.1016/j.ecoenv.2007.05.004 IV Lennquist A, Hedberg D, Lindblad Mårtensson LGE, Kristiansson E, Förlin L, 2009. Colour and melanophore function in rainbow trout after long term exposure to the new antifoulant medetomidine. Under revision. V Lennquist A, Asker N, Kristiansson E, Brenthel A, , Björnsson BTh, Kling P, Larsson DGJ, and Förlin L. 2010. Physiology and gene expression in rainbow trout (Oncorhynchus mykiss) after long term exposure to the new antifoulant medetomidine. Manuscript. 978-91-628-8082-8 http://hdl.handle.net/2077/22081 |
_version_ |
1781057849167708160 |