Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon
Background and aims: The growth hormone-insulin-like growth factor I (GH-IGF-I) system is known to act during sexual maturation of female salmonids, but the specific roles are not known. Somatolactin (SL) is a pituitary hormone closely related to GH and is only found in fish. In some species, includ...
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ftunivgoeteborg:oai:gupea.ub.gu.se:2077/18305 2023-10-29T02:35:00+01:00 Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon Benedet, Susana 2008-10-31T09:10:52Z application/pdf http://hdl.handle.net/2077/18305 eng eng I. Benedet S, Johansson V, Sweeney G, Galay-Burgos M and Björnsson BTh 2005. Cloning of two Atlantic salmon growth hormone receptor isoforms and in vitro ligand-binding response. Fish Physiology and Biochemistry 31: 315-329.::doi::10.1007/s10695-005-2524-y II. Benedet S, Björnsson BTh, Taranger GL and Andersson E 2008. Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon: Seasonal expression profile in pituitary and ovary of maturing female broodstock Reproductive Biology and Endocrinology 6:42.::doi::10.1186/1477-7827-6-42 III. Benedet S, Andersson E, Mittelholzer C, Taranger GL and Björnsson BTh 2008. Concurrent measurement of pituitary growth hormone mRNA and protein content in sexually maturing female Atlantic salmon: Correlation analysis with plasma GH levels. Manuscript IV. Benedet S, Björnsson BTh, Taranger GL and Andersson E 2008. The growth hormone - insulin-like growth factor I system during sexual maturation of female Atlantic salmon. Manuscript 978-91-628-7029-4 http://hdl.handle.net/2077/18305 Growth hormone somatolactin insulin-like growth factor I GH SL IGFI GHR SLR IGFIR ovary maturation reproduction Atlantic salmon spawning pituitary Text Doctoral Theses Doctor of Philosophy 2008 ftunivgoeteborg 2023-10-04T21:07:35Z Background and aims: The growth hormone-insulin-like growth factor I (GH-IGF-I) system is known to act during sexual maturation of female salmonids, but the specific roles are not known. Somatolactin (SL) is a pituitary hormone closely related to GH and is only found in fish. In some species, including salmonids, there are two forms, SLa and SLß. The SL receptor (SLR) has recently been cloned and phylogenetic analysis shows that it is similar to previously cloned GH receptors (GHRs) of non-salmonids. The ligand-specificity of the GHR/SLR is unclear. Little is known about the role of the SLs in sexual maturation of fish. The aim of this thesis has been to increase our knowledge about the regulatory role(s) of both the GH-IGF-I system and of SLs during sexual maturation in female Atlantic salmon. Methods: The cDNA sequences of Atlantic salmon GHRs (two isoforms), SLR, as well as SLa and SLß were obtained with the goals of carrying out a phylogenetic analysis, and of developing molecular tools for analysis of mRNA levels using real time quantitative PCR (RTqPCR). The roles of GH, IGF-I and SL were examined in a 17-month long study on one sea winter Atlantic salmon females. mRNA expression levels of ovarian components of the GH-IGF-I system and SLR and pituitary GH, SLa and SLß were studied by RTqPCR. Levels of GH and IGF-I in plasma, and of GH in the pituitary were measured by radio-immunoassay. Results and Conclusions: The phylogenetic analysis (Paper I and II) of the cloned sequences reveals the placement of Atlantic salmon GHR in the GHR type II clade and SLR in the controversial GHR type I clade (putative SLRs). Concurrent analyses of pituitary GH mRNA levels, GH protein and plasma GH in the same individual fish demonstrates the complex dynamics of the GH system, which is inhibited by a continuous light. Papers III and IV confirm that there is an active GH-IGF-I-gonad axis in the female Atlantic salmon that appears to be functional at the start of exogenous vitellogenesis, final oocyte growth, spawning and ... Doctoral or Postdoctoral Thesis Atlantic salmon University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
institution |
Open Polar |
collection |
University of Gothenburg: GUPEA (Gothenburg University Publications Electronic Archive) |
op_collection_id |
ftunivgoeteborg |
language |
English |
topic |
Growth hormone somatolactin insulin-like growth factor I GH SL IGFI GHR SLR IGFIR ovary maturation reproduction Atlantic salmon spawning pituitary |
spellingShingle |
Growth hormone somatolactin insulin-like growth factor I GH SL IGFI GHR SLR IGFIR ovary maturation reproduction Atlantic salmon spawning pituitary Benedet, Susana Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
topic_facet |
Growth hormone somatolactin insulin-like growth factor I GH SL IGFI GHR SLR IGFIR ovary maturation reproduction Atlantic salmon spawning pituitary |
description |
Background and aims: The growth hormone-insulin-like growth factor I (GH-IGF-I) system is known to act during sexual maturation of female salmonids, but the specific roles are not known. Somatolactin (SL) is a pituitary hormone closely related to GH and is only found in fish. In some species, including salmonids, there are two forms, SLa and SLß. The SL receptor (SLR) has recently been cloned and phylogenetic analysis shows that it is similar to previously cloned GH receptors (GHRs) of non-salmonids. The ligand-specificity of the GHR/SLR is unclear. Little is known about the role of the SLs in sexual maturation of fish. The aim of this thesis has been to increase our knowledge about the regulatory role(s) of both the GH-IGF-I system and of SLs during sexual maturation in female Atlantic salmon. Methods: The cDNA sequences of Atlantic salmon GHRs (two isoforms), SLR, as well as SLa and SLß were obtained with the goals of carrying out a phylogenetic analysis, and of developing molecular tools for analysis of mRNA levels using real time quantitative PCR (RTqPCR). The roles of GH, IGF-I and SL were examined in a 17-month long study on one sea winter Atlantic salmon females. mRNA expression levels of ovarian components of the GH-IGF-I system and SLR and pituitary GH, SLa and SLß were studied by RTqPCR. Levels of GH and IGF-I in plasma, and of GH in the pituitary were measured by radio-immunoassay. Results and Conclusions: The phylogenetic analysis (Paper I and II) of the cloned sequences reveals the placement of Atlantic salmon GHR in the GHR type II clade and SLR in the controversial GHR type I clade (putative SLRs). Concurrent analyses of pituitary GH mRNA levels, GH protein and plasma GH in the same individual fish demonstrates the complex dynamics of the GH system, which is inhibited by a continuous light. Papers III and IV confirm that there is an active GH-IGF-I-gonad axis in the female Atlantic salmon that appears to be functional at the start of exogenous vitellogenesis, final oocyte growth, spawning and ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Benedet, Susana |
author_facet |
Benedet, Susana |
author_sort |
Benedet, Susana |
title |
Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
title_short |
Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
title_full |
Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
title_fullStr |
Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
title_full_unstemmed |
Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon |
title_sort |
growth hormone and somatolactin function during sexual maturation of female atlantic salmon |
publishDate |
2008 |
url |
http://hdl.handle.net/2077/18305 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
I. Benedet S, Johansson V, Sweeney G, Galay-Burgos M and Björnsson BTh 2005. Cloning of two Atlantic salmon growth hormone receptor isoforms and in vitro ligand-binding response. Fish Physiology and Biochemistry 31: 315-329.::doi::10.1007/s10695-005-2524-y II. Benedet S, Björnsson BTh, Taranger GL and Andersson E 2008. Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon: Seasonal expression profile in pituitary and ovary of maturing female broodstock Reproductive Biology and Endocrinology 6:42.::doi::10.1186/1477-7827-6-42 III. Benedet S, Andersson E, Mittelholzer C, Taranger GL and Björnsson BTh 2008. Concurrent measurement of pituitary growth hormone mRNA and protein content in sexually maturing female Atlantic salmon: Correlation analysis with plasma GH levels. Manuscript IV. Benedet S, Björnsson BTh, Taranger GL and Andersson E 2008. The growth hormone - insulin-like growth factor I system during sexual maturation of female Atlantic salmon. Manuscript 978-91-628-7029-4 http://hdl.handle.net/2077/18305 |
_version_ |
1781057920283181056 |