Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use
Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbird...
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ftunivgironadugi:oai:dugi-doc.udg.edu:10256/21311 2023-05-15T17:07:56+02:00 Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use Jarma, Dayana Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas, Francisco Sànchez Melsió, Alexandre Borrego i Moré, Carles 2021-08-20 application/pdf http://hdl.handle.net/10256/21311 eng eng Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2021.146872 info:eu-repo/semantics/altIdentifier/issn/0048-9697 info:eu-repo/semantics/altIdentifier/eissn/1879-1026 http://hdl.handle.net/10256/21311 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Science of The Total Environment, 2021, vol. 83, art. núm. 146872 Articles publicats (ICRA) Resistència als medicaments Drug resistance Bacteris patògens Pathogenic bacteria Ocells -- Patògens Birds -- Pathogens Ocells -- Excrements -- Microbiologia Bird droppings -- Microbiology info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed 2021 ftunivgironadugi https://doi.org/10.1016/j.scitotenv.2021.146872 2022-07-12T23:43:05Z Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbirds wintering in South-West Spain that differ in their habitat use. The white stork Ciconia ciconia and lesser black-backed gull Larus fuscus are omnivorous and opportunistic birds that use highly anthropogenic habitats such as landfills and urban areas. The greylag goose Anser anser and common crane Grus grus are herbivores and use more natural habitats. Fresh faeces from 15 individuals of each species were analysed to assess the composition of bacterial communities using 16S rRNA amplicon-targeted sequencing, and to quantify the abundance of the Class I integron integrase gene (intI1) as well as genes encoding resistance to sulfonamides (sul1), beta-lactams (blaTEM, blaKPC and blaNDM), tetracyclines (tetW), fluoroquinolones (qnrS), and colistin (mcr-1) using qPCR. Bacterial communities in gull faeces were the richest and most diverse. Beta diversity analysis showed segregation in faecal communities between bird species, but those from storks and gulls were the most similar, these being the species that regularly feed in landfills. Potential bacterial pathogens identified in faeces differed significantly between bird species, with higher relative abundance in gulls. Faeces from birds that feed in landfills (stork and gull) contained a significantly higher abundance of ARGs (sul1, blaTEM, and tetW). Genes conferring resistance to last resort antibiotics such as carbapenems (blaKPC) and colistin (mcr-1) were only observed in faeces from gulls. These results show that these bird species are reservoirs of antimicrobial resistant bacteria and suggest that waterbirds may disseminate antibiotic resistance across environments (e.g., from landfills to ricefields or water supplies), and thus constitute a risk for their further ... Article in Journal/Newspaper Lesser black-backed gull Universitat de Girona: DUGiDocs (UdG Digital Repository) Science of The Total Environment 783 146872 |
institution |
Open Polar |
collection |
Universitat de Girona: DUGiDocs (UdG Digital Repository) |
op_collection_id |
ftunivgironadugi |
language |
English |
topic |
Resistència als medicaments Drug resistance Bacteris patògens Pathogenic bacteria Ocells -- Patògens Birds -- Pathogens Ocells -- Excrements -- Microbiologia Bird droppings -- Microbiology |
spellingShingle |
Resistència als medicaments Drug resistance Bacteris patògens Pathogenic bacteria Ocells -- Patògens Birds -- Pathogens Ocells -- Excrements -- Microbiologia Bird droppings -- Microbiology Jarma, Dayana Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas, Francisco Sànchez Melsió, Alexandre Borrego i Moré, Carles Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
topic_facet |
Resistència als medicaments Drug resistance Bacteris patògens Pathogenic bacteria Ocells -- Patògens Birds -- Pathogens Ocells -- Excrements -- Microbiologia Bird droppings -- Microbiology |
description |
Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbirds wintering in South-West Spain that differ in their habitat use. The white stork Ciconia ciconia and lesser black-backed gull Larus fuscus are omnivorous and opportunistic birds that use highly anthropogenic habitats such as landfills and urban areas. The greylag goose Anser anser and common crane Grus grus are herbivores and use more natural habitats. Fresh faeces from 15 individuals of each species were analysed to assess the composition of bacterial communities using 16S rRNA amplicon-targeted sequencing, and to quantify the abundance of the Class I integron integrase gene (intI1) as well as genes encoding resistance to sulfonamides (sul1), beta-lactams (blaTEM, blaKPC and blaNDM), tetracyclines (tetW), fluoroquinolones (qnrS), and colistin (mcr-1) using qPCR. Bacterial communities in gull faeces were the richest and most diverse. Beta diversity analysis showed segregation in faecal communities between bird species, but those from storks and gulls were the most similar, these being the species that regularly feed in landfills. Potential bacterial pathogens identified in faeces differed significantly between bird species, with higher relative abundance in gulls. Faeces from birds that feed in landfills (stork and gull) contained a significantly higher abundance of ARGs (sul1, blaTEM, and tetW). Genes conferring resistance to last resort antibiotics such as carbapenems (blaKPC) and colistin (mcr-1) were only observed in faeces from gulls. These results show that these bird species are reservoirs of antimicrobial resistant bacteria and suggest that waterbirds may disseminate antibiotic resistance across environments (e.g., from landfills to ricefields or water supplies), and thus constitute a risk for their further ... |
format |
Article in Journal/Newspaper |
author |
Jarma, Dayana Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas, Francisco Sànchez Melsió, Alexandre Borrego i Moré, Carles |
author_facet |
Jarma, Dayana Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas, Francisco Sànchez Melsió, Alexandre Borrego i Moré, Carles |
author_sort |
Jarma, Dayana |
title |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
title_short |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
title_full |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
title_fullStr |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
title_full_unstemmed |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
title_sort |
faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://hdl.handle.net/10256/21311 |
genre |
Lesser black-backed gull |
genre_facet |
Lesser black-backed gull |
op_source |
Science of The Total Environment, 2021, vol. 83, art. núm. 146872 Articles publicats (ICRA) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2021.146872 info:eu-repo/semantics/altIdentifier/issn/0048-9697 info:eu-repo/semantics/altIdentifier/eissn/1879-1026 http://hdl.handle.net/10256/21311 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.scitotenv.2021.146872 |
container_title |
Science of The Total Environment |
container_volume |
783 |
container_start_page |
146872 |
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1766063455748489216 |