Large heterotrophic bacterial diversity of Antarctic samples through cultivation
The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the environment of the Princess...
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ftunivgent:oai:archive.ugent.be:978395 2023-06-11T04:05:31+02:00 Large heterotrophic bacterial diversity of Antarctic samples through cultivation Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominic Willems, Anne 2010 https://biblio.ugent.be/publication/978395 http://hdl.handle.net/1854/LU-978395 eng eng https://biblio.ugent.be/publication/978395 http://hdl.handle.net/1854/LU-978395 IPY Oslo Science Conference, Abstracts Earth and Environmental Sciences Antarctica cultivation heterotrophic bacterial diversity conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2010 ftunivgent 2023-05-10T22:51:39Z The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the environment of the Princess Elisabeth Station (Utsteinen, Dronning Maud Land): BB50 and BB115; two samples from the Trans-Antarctic Mountains: TM2 from Forlidas Pond (Pensacola Mountains) and TM4 from Lundström Lake (Shackleton Mountains); a littoral sample PQ1 from Pourquoi-Pas Lake (Pourquoi-Pas Island); samples LA3 (Langhovde Peninsula), SK5 (Skarvsness Peninsula) and WO10 (West Ongul Island) from three lakes at Lützow-Holm Bay, Syowa and sample SO6 from the Schirmacher Oasis. Samples were investigated with a culture-dependent approach using different media (MA, R A, R2A/10 and PYGV) and incubation temperatures (4, 15 and 20°C). Between 254 and 573 isolates were obtained from each sample and were dereplicated with the rep-PCR fingerprinting technique. Representatives of each rep-cluster were used in partial 16S rDNA sequencing for identification. The preliminary results of the 16S rDNA sequencing show a large diversity, distributed over the major phylogenetic groups. Actinobacteria and Alphaproteobacteria are well represented in all samples and Bacteroidetes and Betaproteobacteria are found in lower amounts whereas Deinococci, Gammaproteobacteria and Firmicutes are only isolated from some samples. Despite the large diversity and the fact that some samples originate from the same general area, only little overlap between the samples was observed. Some of the isolates, especially in the Sphingobacteria and Deinococci show low 16S rDNA sequence similarity values with known sequences and may represent new taxa. Conference Object Antarc* Antarctic Antarctica Dronning Maud Land Ongul Island Pourquoi Pas Island Ghent University Academic Bibliography Antarctic Dronning Maud Land Shackleton Pourquoi Pas ENVELOPE(135.783,135.783,-66.083,-66.083) Pourquoi-Pas ENVELOPE(-67.450,-67.450,-67.700,-67.700) Pourquoi-Pas? ENVELOPE(135.750,135.750,-66.200,-66.200) Ongul ENVELOPE(39.533,39.533,-69.017,-69.017) Ongul Island ENVELOPE(39.533,39.533,-69.017,-69.017) Langhovde ENVELOPE(39.733,39.733,-69.217,-69.217) Lützow-Holm Bay ENVELOPE(38.000,38.000,-69.500,-69.500) Pensacola Mountains ENVELOPE(-58.000,-58.000,-83.500,-83.500) Utsteinen ENVELOPE(23.342,23.342,-71.955,-71.955) Pourquoi Pas Island ENVELOPE(-67.500,-67.500,-67.683,-67.683) Forlidas Pond ENVELOPE(-51.318,-51.318,-82.453,-82.453) |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Earth and Environmental Sciences Antarctica cultivation heterotrophic bacterial diversity |
spellingShingle |
Earth and Environmental Sciences Antarctica cultivation heterotrophic bacterial diversity Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominic Willems, Anne Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
topic_facet |
Earth and Environmental Sciences Antarctica cultivation heterotrophic bacterial diversity |
description |
The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the environment of the Princess Elisabeth Station (Utsteinen, Dronning Maud Land): BB50 and BB115; two samples from the Trans-Antarctic Mountains: TM2 from Forlidas Pond (Pensacola Mountains) and TM4 from Lundström Lake (Shackleton Mountains); a littoral sample PQ1 from Pourquoi-Pas Lake (Pourquoi-Pas Island); samples LA3 (Langhovde Peninsula), SK5 (Skarvsness Peninsula) and WO10 (West Ongul Island) from three lakes at Lützow-Holm Bay, Syowa and sample SO6 from the Schirmacher Oasis. Samples were investigated with a culture-dependent approach using different media (MA, R A, R2A/10 and PYGV) and incubation temperatures (4, 15 and 20°C). Between 254 and 573 isolates were obtained from each sample and were dereplicated with the rep-PCR fingerprinting technique. Representatives of each rep-cluster were used in partial 16S rDNA sequencing for identification. The preliminary results of the 16S rDNA sequencing show a large diversity, distributed over the major phylogenetic groups. Actinobacteria and Alphaproteobacteria are well represented in all samples and Bacteroidetes and Betaproteobacteria are found in lower amounts whereas Deinococci, Gammaproteobacteria and Firmicutes are only isolated from some samples. Despite the large diversity and the fact that some samples originate from the same general area, only little overlap between the samples was observed. Some of the isolates, especially in the Sphingobacteria and Deinococci show low 16S rDNA sequence similarity values with known sequences and may represent new taxa. |
format |
Conference Object |
author |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominic Willems, Anne |
author_facet |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominic Willems, Anne |
author_sort |
Peeters, Karolien |
title |
Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_short |
Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_full |
Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_fullStr |
Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_full_unstemmed |
Large heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_sort |
large heterotrophic bacterial diversity of antarctic samples through cultivation |
publishDate |
2010 |
url |
https://biblio.ugent.be/publication/978395 http://hdl.handle.net/1854/LU-978395 |
long_lat |
ENVELOPE(135.783,135.783,-66.083,-66.083) ENVELOPE(-67.450,-67.450,-67.700,-67.700) ENVELOPE(135.750,135.750,-66.200,-66.200) ENVELOPE(39.533,39.533,-69.017,-69.017) ENVELOPE(39.533,39.533,-69.017,-69.017) ENVELOPE(39.733,39.733,-69.217,-69.217) ENVELOPE(38.000,38.000,-69.500,-69.500) ENVELOPE(-58.000,-58.000,-83.500,-83.500) ENVELOPE(23.342,23.342,-71.955,-71.955) ENVELOPE(-67.500,-67.500,-67.683,-67.683) ENVELOPE(-51.318,-51.318,-82.453,-82.453) |
geographic |
Antarctic Dronning Maud Land Shackleton Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Ongul Ongul Island Langhovde Lützow-Holm Bay Pensacola Mountains Utsteinen Pourquoi Pas Island Forlidas Pond |
geographic_facet |
Antarctic Dronning Maud Land Shackleton Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Ongul Ongul Island Langhovde Lützow-Holm Bay Pensacola Mountains Utsteinen Pourquoi Pas Island Forlidas Pond |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land Ongul Island Pourquoi Pas Island |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land Ongul Island Pourquoi Pas Island |
op_source |
IPY Oslo Science Conference, Abstracts |
op_relation |
https://biblio.ugent.be/publication/978395 http://hdl.handle.net/1854/LU-978395 |
_version_ |
1768376783110930432 |